Western Blot (WB)
(Western Blot analysis of extracts of SPDEF antibody.)
Rabbit SPDEF Polyclonal Antibody | anti-SPDEF antibody
SPDEF Polyclonal Antibody
Gene Names
SPDEF; PDEF; bA375E1.3
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
SPDEF; Polyclonal Antibody; SPDEF Polyclonal Antibody; SAM pointed domain-containing Ets transcription factor; Prostate epithelium-specific Ets transcription factor; Prostate-specific Ets; Prostate-derived Ets factor; PDEF; PSE; anti-SPDEF antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
SPDEF polyclonal antibody detects endogenous levels of SPDEF protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
319
Applicable Applications for anti-SPDEF antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
WB: 1:500-1:1000
IHC: 1:50-1:200
IF: 1:50-1:100
IHC: 1:50-1:200
IF: 1:50-1:100
Immunogen
Recombinant full length Human SPDEF.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot analysis of extracts of SPDEF antibody.)
Western Blot (WB)
(Western Blot analysis of extracts of SPDEF antibody.)
Related Product Information for anti-SPDEF antibody
Prostate epithelium-specific Ets transcription factor (PDEF), also designated prostate Ets or SAM pointed domain containing Ets transcription factor, is a 335 amino acid nuclear protein. PDEF belongs to the Ets family of proteins. This protein, which localizes to prostate epithelial cells, functions as an Ets transcription factor. It upregulates the activity of the p62 promotor but this activity can be downregulated by PSI. It is also involved in the activation of prostate-specific antigen (PSA) by acting as an androgen-independent transactivator.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~ 50kDa
NCBI Official Full Name
SAM pointed domain-containing Ets transcription factor isoform 2
NCBI Official Synonym Full Names
SAM pointed domain containing ETS transcription factor
NCBI Official Symbol
SPDEF
NCBI Official Synonym Symbols
PDEF; bA375E1.3
NCBI Protein Information
SAM pointed domain-containing Ets transcription factor
UniProt Protein Name
SAM pointed domain-containing Ets transcription factor
Protein Family
UniProt Gene Name
SPDEF
UniProt Synonym Gene Names
PDEF; PSE; Prostate-specific Ets
NCBI Description
The protein encoded by this gene belongs to the ETS family of transcription factors. It is highly expressed in the prostate epithelial cells, and functions as an androgen-independent transactivator of prostate-specific antigen (PSA) promoter. Higher expression of this protein has also been reported in brain, breast, lung and ovarian tumors, compared to the corresponding normal tissues, and it shows better tumor-association than other cancer-associated molecules, making it a more suitable target for developing specific cancer therapies. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]
Uniprot Description
May function as an androgen-independent transactivator of the prostate-specific antigen (PSA) promoter. Binds to 5'-GGAT-3' DNA sequences. May play a role in the regulation of the prostate gland and/or prostate cancer development. Acts as a transcriptional activator for SERPINB5 promoter.
Research Articles on SPDEF
Similar Products
Product Notes
The SPDEF spdef (Catalog #AAA3011711) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SPDEF Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SPDEF can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:500-1:1000 IHC: 1:50-1:200 IF: 1:50-1:100. Researchers should empirically determine the suitability of the SPDEF spdef for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SPDEF, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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