Rabbit anti-Human Proenkephalin-A Polyclonal Antibody | anti-PEA antibody

Proenkephalin-A (N-term) Rabbit Polyclonal Antibody

Cat. Num. AAA588298

• Gene Names: PENK; PE; PENK-A
• Reactivity: Human
• Applications: Immunoprecipitation, Western Blot
• Synonyms: Proenkephalin-A; Polyclonal Antibody; Proenkephalin-A (N-term) Rabbit Polyclonal Antibody; Proenkephalin; PENK; anti-PEA antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Form/Format: Lyophilized serum
• Sequence Length: 267

• Applicable Applications for anti-PEA antibody: Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: If reconstituted in 100 muL: WB: 1:1000-1:2000; IP 10 pi of serum.
• Immunogen: Immunogen: N-terminal peptide (AA 63-70)
• Preservative: None
• Preparation and Storage: Lyophilized antibody can be kept at 4 degree C for up to 3 months and should be kept at-20 degree C for long-term storage.; To avoid freeze-thaw cycles, reconstituted antibody should be aliquoted before freezing for short-term storage (-20 degree C) or for long-term storage (-80 degree C).; For maximum recovery of product centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-PEA antibody

Vertebrate endogenous opioid neuropeptides are released by post-translational proteolytic cleavage of precursor proteins. Proenkephalin-A is one of the three opioid neuropeptide precursor molecules of the opioid pentapeptides Met-and Leu-enkephalin, which compete with and mimic the effects of opiate drugs. Upon cleavage, each proenkephalin peptide results in the generation of four copies of Met-enkephalin, two extended copies of Met-enkephalin, and one copy of Leu-enkephalin. Proenkephalin opioid neuropeptide is one of the key neurobiological substrates of addiction disorders implicated in reward, mood regulation, stress response, and motor function.

References

1. Goumon Y-Processing of proenkephalin-A in bovine chromaffin cells. Identification of natural derived fragments by N-terminal sequencing and matrix-assisted.

NCBI and Uniprot Product Information

• NCBI GI #: 208879446
• NCBI GeneID: 5179
• NCBI Accession #: NP_001129162.1
• NCBI GenBank Nucleotide #: NM_001135690.3
• UniProt Accession #: P01210; Q6FHC6; Q6FHE6; B2RC23
• Molecular Weight: 30,787 Da
• NCBI Official Full Name: proenkephalin-A preproprotein
• NCBI Official Synonym Full Names: proenkephalin
• NCBI Official Symbol: PENK
• NCBI Official Synonym Symbols: PE; PENK-A
• NCBI Protein Information: proenkephalin-A
• UniProt Protein Name: Proenkephalin-A
• Protein Family: Proenkephalin
• UniProt Gene Name: PENK
• UniProt Synonym Gene Names: OGF

NCBI Description

This gene encodes a preproprotein that is proteolytically processed to generate multiple protein products. These products include the pentapeptide opioids Met-enkephalin and Leu-enkephalin, which are stored in synaptic vesicles, then released into the synapse where they bind to mu- and delta-opioid receptors to modulate the perception of pain. Other non-opioid cleavage products may function in distinct biological activities. [provided by RefSeq, Jul 2015]

Uniprot Description

Met- and Leu-enkephalins compete with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress. PENK(114-133) and PENK(237-258) increase glutamate release in the striatum. PENK(114-133) decreases GABA concentration in the striatum.