Rabbit anti-Mouse CAR/CXADR Polyclonal Antibody | anti-CXADR antibody

Anti-CAR/CXADR Antibody, Rabbit Polyclonal

Cat. Num. AAA8110784

• Gene Names: Cxadr; CAR; MCAR; MCVADR; AU016810; AW553441; 2610206D03Rik
• Reactivity: Mouse
• Applications: ELISA, Immunohistochemistry
• Purity: Protein A & Antigen Affinity
• Synonyms: CAR/CXADR; Polyclonal Antibody; Anti-CAR/CXADR Antibody; Rabbit Polyclonal; CXADR Antibody; Rabbit PAb; Antigen Affinity Purified; coxsackie virus and adenovirus receptor; Anti-2610206D03Rik Antibody; Anti-AU016810 Antibody; Anti-AW553441 Antibody; Anti-CAR Antibody; Anti-MCAR Antibody; Anti-MCVADR Antibody; anti-CXADR antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Mouse CXADR
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-CXADR antibody: ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: ELISA: 1:5000-1:10,000; IHC-P: 1:500-1:2000
• Immunogen: Recombinant Mouse CXADR protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Mouse CXADR (rM CXADR). CXADR specific IgG was purified by Mouse CXADR affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of mouse CXADR in mouse stomach with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)

Immunohistochemistry (IHC)

(Immunochemical staining of mouse CXADR in mouse liver with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections). The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).)

Related Product Information for anti-CXADR antibody

CXADR (coxsackie virus and adenovirus receptor), also known as CAR, is a type I transmembrane glycoprotein belonging to the CTX family of the Ig superfamily, and is essential for normal cardiac development in the mouse. Proposed as a homophilic cell adhesion molecule, CXADR is a component of the epithelial apical junction complex that is essential for the tight junction integrity, and probably involved in transepithelial migration of polymorphonuclear leukocytes (PMN). Mature mouse CXADR structrually comprises a 218 aa extracellular domain (ECD) with a V-type (D1) and a C2-type (D2) Ig-like domain, a 21 aa transmembrane segment and a 17 aa intracellular domain, among which, D1 is thought to be responsible for homodimer formation in trans within tight junctions. The ECD of mouse CXADR shares 97%, 9% sequence identity with the corresponding regions of rat, human CXADR.

References

Tomko, R.P. et al., 1997, Proc. Natl. Acad. Sci. U.S.A. 94 (7): 3352-3356. van Raaij, M.J. et al., 2001, Structure. 8 (11): 1147-1155. Cohen, C.J. et al., 2001, J. Biol. Chem. 276 (27): 25392-25398. Carson, S.D. et al., 2002, Rev. Med. Virol. 11 (4): 219-226. Selinka, H.C. et al., 2004, Med. Microbiol. Immunol. 193 (2-3): 127-131. Philipson, L. et al., 2004, Curr. Top. Microbiol. Immunol. 273:87-111. Raschperger, E. et al., 2006, Exp. Cell Res. 312: 1566-1580.

NCBI and Uniprot Product Information

• NCBI GI #: 68510034
• NCBI GeneID: 13052
• NCBI Accession #: NP_001020363.1
• NCBI GenBank Nucleotide #: NM_001025192.3
• UniProt Accession #: P97792; O09052; Q3ULD0; Q91W66; Q99KG0; Q9DBJ8
• Molecular Weight: 17,781 Da
• NCBI Official Full Name: coxsackievirus and adenovirus receptor homolog isoform a
• NCBI Official Synonym Full Names: coxsackie virus and adenovirus receptor
• NCBI Official Symbol: Cxadr
• NCBI Official Synonym Symbols: CAR; MCAR; MCVADR; AU016810; AW553441; 2610206D03Rik
• NCBI Protein Information: coxsackievirus and adenovirus receptor homolog
• UniProt Protein Name: Coxsackievirus and adenovirus receptor homolog
• Protein Family: Coxsackievirus and adenovirus receptor
• UniProt Gene Name: Cxadr
• UniProt Synonym Gene Names: Car; CAR; mCAR

NCBI Description

This gene encodes a protein that is part of the Cortical Thymocyte marker in Xenopus (CTX) subfamily within the immunoglobulin superfamily. Members of this subfamily, predominantly expressed on the surface of endothelial and epithelial cells, help establish cell polarity and provide a barrier function, regulating migration of immune cells. This protein, first identified as the receptor for adenovirus subgroup C and coxsakieviruses group B, is developmentally regulated and plays an important role in cardiac development. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Jan 2013]

Uniprot Description

Component of the epithelial apical junction complex that may function as a homophilic cell adhesion molecule and is essential for tight junction integrity. Also involved in transepithelial migration of leukocytes through adhesive interactions with JAML a transmembrane protein of the plasma membrane of leukocytes. The interaction between both receptors also mediates the activation of gamma-delta T-cells, a subpopulation of T-cells residing in epithelia and involved in tissue homeostasis and repair. Upon epithelial CXADR-binding, JAML induces downstream cell signaling events in gamma-delta T-cells through PI3-kinase and MAP kinases. It results in proliferation and production of cytokines and growth factors by T-cells that in turn stimulate epithelial tissues repair.