Human Proenkephalin (PENK) ELISA Kit | PENK elisa kit

Human Proenkephalin (PENK) ELISA Kit

Cat. Num. AAA9714916

• Gene Names: PENK; PE; PENK-A
• Reactivity: Human
• Synonyms: Proenkephalin (PENK); Human Proenkephalin (PENK) ELISA Kit; PENK; Enkephalin A; preproenkephalin; PENK elisa kit

• Reactivity: Human
• Sequence Length: 267

• Assay Type: Sandwich
• Preparation and Storage: Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PENK elisa kit

Principle of the Assay: Human Proenkephalin (PENK) ELISA Kit employs a two-site sandwich ELISA to quantitate PENK in samples. An antibody specific for PENK has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PENK present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugated Human PENK detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of PENK bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background/Introduction: Preproenkephalin has 267 amino acids, as does proopiomelanocortin. Both genes contain 2 introns. In both, all the repeated enkephalin or melanotropin sequences are encoded by a single large exon (exon 3). Preproenkephalin mRNA encodes 4 copies of met-enkephalin, 2 copies of met-enkephalin extended sequences, and 1 copy of leu-enkephalin. Each copy is flanked by paired basic amino acids which are presumably recognized by the processing protease. The corresponding amino acid sequence shows that the precursor is 267 amino acids long and contains 6 interspersed Met-enkephalin sequences and 1 Leu-enkephalin sequence. The precursor does not contain the sequences of dynorphin, alpha-neo-endorphin or beta-endorphin.

NCBI and Uniprot Product Information

• NCBI GI #: 208879446
• NCBI GeneID: 5179
• NCBI Accession #: NP_001129162.1
• NCBI GenBank Nucleotide #: NP_001129162.1
• UniProt Accession #: P01210
• NCBI Official Full Name: proenkephalin-A preproprotein
• NCBI Official Synonym Full Names: proenkephalin
• NCBI Official Symbol: PENK
• NCBI Official Synonym Symbols: PE; PENK-A
• NCBI Protein Information: proenkephalin-A
• UniProt Protein Name: Proenkephalin-A
• Protein Family: Proenkephalin
• UniProt Gene Name: PENK
• UniProt Synonym Gene Names: OGF
• UniProt Entry Name: PENK_HUMAN

NCBI Description

This gene encodes a preproprotein that is proteolytically processed to generate multiple protein products. These products include the pentapeptide opioids Met-enkephalin and Leu-enkephalin, which are stored in synaptic vesicles, then released into the synapse where they bind to mu- and delta-opioid receptors to modulate the perception of pain. Other non-opioid cleavage products may function in distinct biological activities. [provided by RefSeq, Jul 2015]

Uniprot Description

PENK: Met- and Leu-enkephalins compete with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress. PENK(114-133) and PENK(237-258) increase glutamate release in the striatum. PENK(114-133) decreases GABA concentration in the striatum. Belongs to the opioid neuropeptide precursor family.

Protein type: Secreted; Secreted, signal peptide; Hormone

Chromosomal Location of Human Ortholog: 8q23-q24

Cellular Component: extracellular region

Molecular Function: neuropeptide hormone activity; protein binding; opioid peptide activity

Biological Process: startle response; behavioral fear response; neuropeptide signaling pathway; locomotory behavior; sensory perception of pain; signal transduction