Flow Cytometry FC
(Overlay histogram showing A549 cells stainedwith MBS9207635 (green line). The cells were fixedwith 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min.The cells were then icubated in 2% bovine serum albumin to block non-specificprotein-protein interactions followed by the antibody (MBS9207635, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used wasGoat-Anti-Rabbit IgG, DyLight® 488Conjugated Highly Cross-Adsorbed(OH191631)at 1/200 dilution for 40 min at 37ºC. Isotypecontrol antibody (blue line) was rabbit IgG(1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
Rabbit anti-Human, mouse PRODH Polyclonal Antibody | anti-Prodh antibody
PRODH Antibody (Center)
IHC-P~~1:50~100
WB~~1:1000
Flow Cytometry FC
(Overlay histogram showing A549 cells stainedwith MBS9207635 (green line). The cells were fixedwith 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min.The cells were then icubated in 2% bovine serum albumin to block non-specificprotein-protein interactions followed by the antibody (MBS9207635, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used wasGoat-Anti-Rabbit IgG, DyLight® 488Conjugated Highly Cross-Adsorbed(OH191631)at 1/200 dilution for 40 min at 37ºC. Isotypecontrol antibody (blue line) was rabbit IgG(1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
Flow Cytometry FC
(Overlay histogram showing A549 cells stainedwith MBS9207635 (green line). The cells were fixedwith 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min.The cells were then icubated in 2% bovine serum albumin to block non-specificprotein-protein interactions followed by the antibody (MBS9207635, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used wasGoat-Anti-Rabbit IgG, DyLight® 488Conjugated Highly Cross-Adsorbed(OH191631)at 1/200 dilution for 40 min at 37ºC. Isotypecontrol antibody (blue line) was rabbit IgG(1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
Immunohistochemistry IHC
( MBS9207635_IHC staining PRODH in H. skeletal musclesections by Immunohistochemistry (IHC-P paraformaldehyde-fixed, paraffin-embeddedsections). Tissue was fixed with formaldehydeand blocked with 3% BSA for 0. 5 hour at roomtemperature; antigen retrieval was by heatmediation with a citrate buffer (pH6). Sampleswere incubated with primary antibody (1/25)for 1 hours at 37°C. A undiluted biotinylatedgoat polyvalent antibody was used as the secondary antibody.)
Immunohistochemistry IHC
( MBS9207635_IHC staining PRODH in H. skeletal musclesections by Immunohistochemistry (IHC-P paraformaldehyde-fixed, paraffin-embeddedsections). Tissue was fixed with formaldehydeand blocked with 3% BSA for 0. 5 hour at roomtemperature; antigen retrieval was by heatmediation with a citrate buffer (pH6). Sampleswere incubated with primary antibody (1/25)for 1 hours at 37°C. A undiluted biotinylatedgoat polyvalent antibody was used as the secondary antibody.)
Western Blot WB
( Anti-PRODH Antibody (Center) at 1: 2000 dilution + MDA-MB-468 whole cell lysateLysates/proteins at 20 µg per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidaseconjugated at 1/10000 dilution. Predicted bandsize : 68 kDa Blocking/Dilution buffer: 5%NFDM/TBST.)
Western Blot WB
( Anti-PRODH Antibody (Center) at 1: 2000 dilution + MDA-MB-468 whole cell lysateLysates/proteins at 20 µg per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidaseconjugated at 1/10000 dilution. Predicted bandsize : 68 kDa Blocking/Dilution buffer: 5%NFDM/TBST.)
Testing Data
( Anti-PRODH Antibody (Center) at 1:2000dilution + human brain lysate Lysates/proteinsat 20 µg per lane. Secondary Goat Anti-RabbitIgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 68 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Testing Data
( Anti-PRODH Antibody (Center) at 1:2000dilution + human brain lysate Lysates/proteinsat 20 µg per lane. Secondary Goat Anti-RabbitIgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 68 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot WB
(All lanes : Anti-PRODH Antibody (Center) at1:1000 dilution Lane 1: MDA-MB-468 whole celllysates Lane 2: A549 whole cell lysatesLysates/proteins at 20 µg per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted bandsize : 68 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot WB
(All lanes : Anti-PRODH Antibody (Center) at1:1000 dilution Lane 1: MDA-MB-468 whole celllysates Lane 2: A549 whole cell lysatesLysates/proteins at 20 µg per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted bandsize : 68 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(Western blot analysis of mouse Prodh Antibody (Center) in mouse liver, kidney tissue lysates (35ug/lane). PRODH (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of mouse Prodh Antibody (Center) in mouse liver, kidney tissue lysates (35ug/lane). PRODH (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human brain tissue reacted with mouse Prodh Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human brain tissue reacted with mouse Prodh Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
NCBI and Uniprot Product Information
Uniprot Description
PRODH: Converts proline to delta-1-pyrroline-5-carboxylate. Defects in PRODH are the cause of hyperprolinemia type 1 (HP-1). HP-1 is a disorder characterized by elevated serum proline levels. May be involved in the psychiatric and behavioral phenotypes associated with the 22q11 velocardiofacial and DiGeorge syndrome. Defects in PRODH are associated with susceptibility to schizophrenia type 4 (SCZD4). A complex, multifactorial psychotic disorder or group of disorders characterized by disturbances in the form and content of thought (e.g. delusions, hallucinations), in mood (e.g. inappropriate affect), in sense of self and relationship to the external world (e.g. loss of ego boundaries, withdrawal), and in behavior (e.g bizarre or apparently purposeless behavior). Although it affects emotions, it is distinguished from mood disorders in which such disturbances are primary. Similarly, there may be mild impairment of cognitive function, and it is distinguished from the dementias in which disturbed cognitive function is considered primary. Some patients manifest schizophrenic as well as bipolar disorder symptoms and are often given the diagnosis of schizoaffective disorder. Belongs to the proline oxidase family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Oxidoreductase; Mitochondrial; EC 1.5.5.2; Amino Acid Metabolism - arginine and proline
Cellular Component: mitochondrion; mitochondrial inner membrane
Molecular Function: amino acid binding; proline dehydrogenase activity; oxidoreductase activity
Biological Process: proline catabolic process; proline metabolic process
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Product Notes
The Prodh prodh (Catalog #AAA9207635) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 130-155. The PRODH Antibody (Center) reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PRODH can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC), Immunohistochemistry-Paraffin (IHC-P), Western Blot (WB), ELISA (EIA). FC~~1:25IHC-P~~1:50~100
WB~~1:1000. Researchers should empirically determine the suitability of the Prodh prodh for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PRODH, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.
Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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