Rabbit PRODH Polyclonal Antibody | anti-PRODH antibody

PRODH Polyclonal Antibody

Cat. Num. AAA3011364

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: PRODH; Polyclonal Antibody; PRODH Polyclonal Antibody; HSPOX2; mitochondrial; P53 induced gene 6 protein; p53-induced gene 6 protein; PIG6; PROD_HUMAN; PRODH 1; PRODH 2; PRODH1; PRODH2; Proline dehydrogenase; proline dehydrogenase (oxidase) 1; proline dehydrogenase (proline oxidase); Proline dehydrogenase 1; Proline oxidase 2; Proline oxidase; mitochondrial precursor; SCZD4; TP53I6; tumor protein p53 inducible protein 6; anti-PRODH antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: PRODH polyclonal antibody detects endogenous levels of PRODH protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 600

• Applicable Applications for anti-PRODH antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200
• Immunogen: Recombinant full length Human PRODH.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of PRODH polyclonal antibody)

Related Product Information for anti-PRODH antibody

PRODH (proline dehydrogenase), also known as proline oxidase 2 (POX2), is a 516 amino acid protein belonging to the proline oxidase family. Induced during p53-induced apoptosis, PRODH catalyzes the first step in proline degradation by converting proline to delta-1-pyrroline-5-carboxylate. Defects in PRODH are the cause of hyperprolinemia type 1, a disorder characterized by elevated serum proline levels. Defects in PRODH may also be involved in the psychiatric and behavioral phenotypes associated with DiGeorge syndrome. Localized to the mitochondrion matrix, PRODH is expressed at higher levels in lung, brain and skeletal muscle and expressed at lower levels in heart, liver, kidney and pancreas. PRODH exists as two isoforms produced by alternative splicing.

NCBI and Uniprot Product Information

• NCBI GI #: 183227691
• NCBI Accession #: NP_057419.3
• NCBI GenBank Nucleotide #: NP_057419.3
• UniProt Accession #: O43272
• Molecular Weight: ~ 68kDa
• NCBI Official Full Name: proline dehydrogenase, mitochondrial
• UniProt Protein Name: Proline dehydrogenase 1, mitochondrial
• UniProt Gene Name: PRODH
• UniProt Synonym Gene Names: PIG6; POX2
• UniProt Entry Name: PROD_HUMAN

Uniprot Description

PRODH: Converts proline to delta-1-pyrroline-5-carboxylate. Defects in PRODH are the cause of hyperprolinemia type 1 (HP-1). HP-1 is a disorder characterized by elevated serum proline levels. May be involved in the psychiatric and behavioral phenotypes associated with the 22q11 velocardiofacial and DiGeorge syndrome. Defects in PRODH are associated with susceptibility to schizophrenia type 4 (SCZD4). A complex, multifactorial psychotic disorder or group of disorders characterized by disturbances in the form and content of thought (e.g. delusions, hallucinations), in mood (e.g. inappropriate affect), in sense of self and relationship to the external world (e.g. loss of ego boundaries, withdrawal), and in behavior (e.g bizarre or apparently purposeless behavior). Although it affects emotions, it is distinguished from mood disorders in which such disturbances are primary. Similarly, there may be mild impairment of cognitive function, and it is distinguished from the dementias in which disturbed cognitive function is considered primary. Some patients manifest schizophrenic as well as bipolar disorder symptoms and are often given the diagnosis of schizoaffective disorder. Belongs to the proline oxidase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Amino Acid Metabolism - arginine and proline; Oxidoreductase; Mitochondrial; EC 1.5.5.2

Chromosomal Location of Human Ortholog: 22q11.21

Cellular Component: mitochondrial matrix; mitochondrial inner membrane

Molecular Function: proline dehydrogenase activity

Biological Process: proline catabolic process; 4-hydroxyproline catabolic process; induction of apoptosis by oxidative stress; proline catabolic process to glutamate; proline metabolic process

Disease: Hyperprolinemia, Type I; Schizophrenia 4