Rabbit anti-Human ALDH4A1 Polyclonal Antibody | anti-ALDH4A1 antibody

ALDH4A1 (Aldehyde Dehydrogenase Family 4 Member A1, Delta-1-pyrroline-5-carboxylate Dehydrogenase, Mitochondrial, P5C Dehydrogenase, ALDH4, P5CDH) (FITC)

Cat. Num. AAA6369498

• Gene Names: ALDH4A1; P5CD; ALDH4; P5CDh
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ALDH4A1; Polyclonal Antibody; ALDH4A1 (Aldehyde Dehydrogenase Family 4 Member A1; Delta-1-pyrroline-5-carboxylate Dehydrogenase; Mitochondrial; P5C Dehydrogenase; ALDH4; P5CDH) (FITC); anti-ALDH4A1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ALDH4A1.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
• Sequence Length: 563

• Applicable Applications for anti-ALDH4A1 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human ALDH4A1, aa1-563 (NP_003739.2).
• Immunogen Sequence: MLLPAPALRRALLSRPWTGAGLRWKHTSSLKVANEPVLAFTQGSPERDALQKALKDLKGRMEAIPCVVGDEEVWTSDVQYQVSPFNHGHKVAKFCYADKSLLNKAIEAALAARKEWDLKPIADRAQIFLKAADMLSGPRRAEILAKTMVGQGKTVIQAEIDAAAELIDFFRFNAKYAVELEGQQPISVPPSTNSTVYRGLEGFVAAISPFNFTAIGGNLAGAPALMGNVVLWKPSDTAMLASYAVYRILREAGLPPNIIQFVPADGPLFGDTVTSSEHLCGINFTGSVPTFKHLWKQVAQNLDRFHTFPRLAGECGGKNFHFVHRSADVESVVSGTLRSAFEYGGQKCSACSRLYVPHSLWPQIKGRLLEEHSRIKVGDPAEDFGTFFSAVIDAKSFARIKKWLEHARSSPSLTILAGGKCDDSVGYFVEPCIVESKDPQEPIMKEEIFGPVLSVYVYPDDKYKETLQLVDSTTSYGLTGAVFSQDKDVVQEATKVLRNAAGNFYINDKSTGSIVGQQPFGGARASGTNDKPGGPHYILRWTSPQVIKETHKPLGDWSYAYMQ
• Conjugate: FITC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(ALDH4A1 rabbit polyclonal antibody. Western Blot analysis of ALDH4A1 expression in HeLa.)

Western Blot (WB)

(Western Blot analysis of ALDH4A1 expression in transfected 293T cell line by ALDH4A1 polyclonal antibody. Lane 1: ALDH4A1 transfected lysate (61.7kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-ALDH4A1 antibody

ALDH4A1 belongs to the aldehyde dehydrogenase family of proteins. This enzyme is a mitochondrial matrix NAD-dependent dehydrogenase which catalyzes the second step of the proline degradation pathway, converting pyrroline-5-carboxylate to glutamate. Deficiency of this enzyme is associated with type II hyperprolinemia, an autosomal recessive disorder characterized by accumulation of delta-1-pyrroline-5-carboxylate (P5C) and proline.

Product Categories/Family for anti-ALDH4A1 antibody

Antibodies; Enzymes; Dehydrogenase

NCBI and Uniprot Product Information

• NCBI GI #: 25777734
• NCBI GeneID: 8659
• NCBI Accession #: NP_003739.2
• NCBI GenBank Nucleotide #: NM_003748.2
• UniProt Accession #: P30038
• NCBI Official Full Name: delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial isoform a
• NCBI Official Synonym Full Names: aldehyde dehydrogenase 4 family member A1
• NCBI Official Symbol: ALDH4A1
• NCBI Official Synonym Symbols: P5CD; ALDH4; P5CDh
• NCBI Protein Information: delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial
• UniProt Protein Name: Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial
• UniProt Gene Name: ALDH4A1
• UniProt Synonym Gene Names: ALDH4; P5CDH; P5C dehydrogenase
• UniProt Entry Name: AL4A1_HUMAN

NCBI Description

This protein belongs to the aldehyde dehydrogenase family of proteins. This enzyme is a mitochondrial matrix NAD-dependent dehydrogenase which catalyzes the second step of the proline degradation pathway, converting pyrroline-5-carboxylate to glutamate. Deficiency of this enzyme is associated with type II hyperprolinemia, an autosomal recessive disorder characterized by accumulation of delta-1-pyrroline-5-carboxylate (P5C) and proline. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jun 2009]

Uniprot Description

ALDH4A1: Irreversible conversion of delta-1-pyrroline-5- carboxylate (P5C), derived either from proline or ornithine, to glutamate. This is a necessary step in the pathway interconnecting the urea and tricarboxylic acid cycles. The preferred substrate is glutamic gamma-semialdehyde, other substrates include succinic, glutaric and adipic semialdehydes. Defects in ALDH4A1 are the cause of hyperprolinemia type 2 (HP-2). HP-2 is characterized by the accumulation of delta-1-pyrroline-5-carboxylate (P5C) and proline. The disorder may be causally related to neurologic manifestations, including seizures and mental retardation. Belongs to the aldehyde dehydrogenase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Amino Acid Metabolism - arginine and proline; Mitochondrial; EC 1.2.1.88; Amino Acid Metabolism - alanine, aspartate and glutamate; Oxidoreductase

Chromosomal Location of Human Ortholog: 1p36

Cellular Component: mitochondrial matrix

Molecular Function: identical protein binding; aldehyde dehydrogenase (NAD) activity; electron carrier activity; 1-pyrroline-5-carboxylate dehydrogenase activity

Biological Process: proline catabolic process; 4-hydroxyproline catabolic process; proline biosynthetic process; glutamate biosynthetic process; proline catabolic process to glutamate; proline metabolic process

Disease: Hyperprolinemia, Type Ii