Rabbit anti-Human PRMT6 Polyclonal Antibody | anti-PRMT6 antibody

Anti-PRMT6 Antibody, Rabbit Polyclonal

Cat. Num. AAA8105270

• Gene Names: PRMT6; HRMT1L6
• Reactivity: Human
• Applications: Western Blot, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: PRMT6; Polyclonal Antibody; Anti-PRMT6 Antibody; Rabbit Polyclonal; PRMT6/HRMT1L6 Antibody; Rabbit PAb; Antigen Affinity Purified; protein arginine methyltransferase 6; Anti-HRMT1L6 Antibody; anti-PRMT6 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human PRMT6/HRMT1L6
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-PRMT6 antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; IP: 1-4uL/mg of lysate
• Immunogen: Recombinant Human PRMT6 protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human PRMT6 (rh PRMT6). PRMT6 specific IgG was purified by human PRMT6 affinity chromatography .
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunoprecipitation (IP)

(PRMT6 was immunoprecipitated using:Lane A:0.5 mg MCF-7 Whole Cell LysateLane B:0.5 mg 293T Whole Cell LysateLane C:0.5 mg Hela Whole Cell Lysate2 uL anti-PRMT6 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-PRMT6 rabbit polyclonal antibody,at 1:200 dilution Secondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:500 dilutionDeveloped using the DAB staining technique.Performed under reducing conditions.Predicted band size: 42 kDaObserved band size: 42 kDa)

Western Blot (WB)

(Anti-PRMT6 rabbit polyclonal antibody at 1:500 dilutionLane A: 293T Whole Cell LysateLane B: MCF7 Whole Cell LysateLane C: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:42 kDaObserved band size:42 kDa)

Related Product Information for anti-PRMT6 antibody

Protein arginine N-methyltransferase 6, also known as Histone-arginine N-methyltransferase PRMT6, PRMT6, and HRMT1L6, is a member of the protein arginine N-methyltransferase family and PRMT6 subfamily. PRMT6 is highly expressed in kidney and testes. PRMT6 is known to catalyze the generation of asymmetric dimethylarginine in polypeptides. It has been implicated in human immunodeficiency virus pathogenesis, DNA repair, and transcriptional regulation. PRMT6 is known to methylate histone H3 Arg-2 (H3R2), and this negatively regulates the lysine methylation of H3K4 resulting in gene repression. PRMT6 plays a key role in coupling process by functioning as a transcriptional coactivator that can regulate alternative splicing. PRMT6 coactivates the progesterone, glucocorticoid and oestrogen receptors in luciferase reporter assays in a hormone-dependent manner. Small interfering RNA (siRNA) oligonucleotide duplex knockdown of PRMT6 disrupts oestrogen-stimulated transcription of endogenous GREB1 and progesterone receptor in MCF-7 breast cancer cells. Neutralizing the activity of PRMT6 could inhibit tumor progression and may be of cancer therapeutic significance.

References

Hyllus D, et al., 2007, Genes Dev. 21(24): 3369-80. Lakowski, TM. et al., 2008, J Biol Chem. 283 (15): 10015-25. Michaud-Levesque, J. et al., 2009, J Biol Chem. 284 (32): 21338-46. Harrison, MJ. et al., 2010, Nucleic acids Res. Jan 4.

NCBI and Uniprot Product Information

• NCBI GI #: 227908867
• NCBI GeneID: 55170
• NCBI Accession #: NP_060607.2
• NCBI GenBank Nucleotide #: NM_018137.2
• UniProt Accession #: Q96LA8; Q5T5Y5; Q6DKI4; Q9NVR8; A3KME1; B4DID8
• Molecular Weight: 32,509 Da
• NCBI Official Full Name: protein arginine N-methyltransferase 6
• NCBI Official Synonym Full Names: protein arginine methyltransferase 6
• NCBI Official Symbol: PRMT6
• NCBI Official Synonym Symbols: HRMT1L6
• NCBI Protein Information: protein arginine N-methyltransferase 6; HMT1 hnRNP methyltransferase-like 6; histone-arginine N-methyltransferase PRMT6; heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 6
• UniProt Protein Name: Protein arginine N-methyltransferase 6
• Protein Family: Protein arginine N-methyltransferase
• UniProt Gene Name: PRMT6
• UniProt Synonym Gene Names: HRMT1L6
• UniProt Entry Name: ANM6_HUMAN

NCBI Description

The protein encoded by this gene belongs to the arginine N-methyltransferase family, which catalyze the sequential transfer of methyl group from S-adenosyl-L-methionine to the side chain nitrogens of arginine residues within proteins, to form methylated arginine derivatives and S-adenosyl-L-homocysteine. This protein can catalyze both, the formation of omega-N monomethylarginine and asymmetrical dimethylarginine, with a strong preference for the latter. It specifically mediates the asymmetric dimethylation of Arg2 of histone H3, and the methylated form represents a specific tag for epigenetic transcriptional repression. This protein also forms a complex with, and methylates DNA polymerase beta, resulting in stimulation of polymerase activity by enhancing DNA binding and processivity. [provided by RefSeq, Sep 2011]

Uniprot Description

PRMT6: Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and asymmetrical dimethylarginine (aDMA), with a strong preference for the formation of aDMA. Preferentially methylates arginyl residues present in a glycine and arginine-rich domain and displays preference for monomethylated substrates. Specifically mediates the asymmetric dimethylation of histone H3 'Arg-2' to form H3R2me2a. H3R2me2a represents a specific tag for epigenetic transcriptional repression and is mutually exclusive with methylation on histone H3 'Lys-4' (H3K4me2 and H3K4me3). It thereby acts as a transcription corepressor of various genes such as HOXA2. Also methylates histone H2A and H4 'Arg-3' (H2AR3me and H4R3me, respectively). Acts as a regulator of DNA base excision during DNA repair by mediating the methylation of DNA polymerase beta (POLB), leading to stimulate the polymerase activity by enhancing DNA binding and processivity. Methylates HMGA1. May play a role in innate immunity against HIV-1 in case of infection by methylating and impairing the function of various HIV-1 proteins such as Tat, Rev and Nucleocapsid protein p7 (NC). Belongs to the protein arginine N-methyltransferase family. PRMT6 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Methyltransferase; EC 2.1.1.125; Methyltransferase, protein arginine

Chromosomal Location of Human Ortholog: 1p13.3

Cellular Component: nucleoplasm; nucleus; cytosol

Molecular Function: histone methyltransferase activity; protein-arginine omega-N asymmetric methyltransferase activity; protein-arginine omega-N monomethyltransferase activity; protein binding; histone binding

Biological Process: histone methylation; establishment and/or maintenance of chromatin architecture; viral reproduction; transcription, DNA-dependent; base-excision repair; peptidyl-arginine methylation, to asymmetrical-dimethyl arginine; negative regulation of transcription, DNA-dependent