Immunofluorescence (IF)
(Immunofluorescence staining of PRMT6 in HeLa cells. Cells were fixed with 4% PFA,blocked with 10% serum. Then incubated with rabbit anti-human PRMT6 polyclonal antibody (1:1000)(Figure A), incubated with rabbit anti-human PRMT6 polyclonal antibody and antigen (Figure B) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to nucleus.)
Rabbit anti-Human PRMT6 Polyclonal Antibody | anti-PRMT6 antibody
Anti-PRMT6 Antibody, Rabbit Polyclonal
Immunofluorescence (IF)
(Immunofluorescence staining of PRMT6 in HeLa cells. Cells were fixed with 4% PFA,blocked with 10% serum. Then incubated with rabbit anti-human PRMT6 polyclonal antibody (1:1000)(Figure A), incubated with rabbit anti-human PRMT6 polyclonal antibody and antigen (Figure B) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to nucleus.)
Immunofluorescence (IF)
(Immunofluorescence staining of PRMT6 in HeLa cells. Cells were fixed with 4% PFA,blocked with 10% serum. Then incubated with rabbit anti-human PRMT6 polyclonal antibody (1:1000)(Figure A), incubated with rabbit anti-human PRMT6 polyclonal antibody and antigen (Figure B) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to nucleus.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene belongs to the arginine N-methyltransferase family, which catalyze the sequential transfer of methyl group from S-adenosyl-L-methionine to the side chain nitrogens of arginine residues within proteins, to form methylated arginine derivatives and S-adenosyl-L-homocysteine. This protein can catalyze both, the formation of omega-N monomethylarginine and asymmetrical dimethylarginine, with a strong preference for the latter. It specifically mediates the asymmetric dimethylation of Arg2 of histone H3, and the methylated form represents a specific tag for epigenetic transcriptional repression. This protein also forms a complex with, and methylates DNA polymerase beta, resulting in stimulation of polymerase activity by enhancing DNA binding and processivity. [provided by RefSeq, Sep 2011]
Uniprot Description
PRMT6: Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and asymmetrical dimethylarginine (aDMA), with a strong preference for the formation of aDMA. Preferentially methylates arginyl residues present in a glycine and arginine-rich domain and displays preference for monomethylated substrates. Specifically mediates the asymmetric dimethylation of histone H3 'Arg-2' to form H3R2me2a. H3R2me2a represents a specific tag for epigenetic transcriptional repression and is mutually exclusive with methylation on histone H3 'Lys-4' (H3K4me2 and H3K4me3). It thereby acts as a transcription corepressor of various genes such as HOXA2. Also methylates histone H2A and H4 'Arg-3' (H2AR3me and H4R3me, respectively). Acts as a regulator of DNA base excision during DNA repair by mediating the methylation of DNA polymerase beta (POLB), leading to stimulate the polymerase activity by enhancing DNA binding and processivity. Methylates HMGA1. May play a role in innate immunity against HIV-1 in case of infection by methylating and impairing the function of various HIV-1 proteins such as Tat, Rev and Nucleocapsid protein p7 (NC). Belongs to the protein arginine N-methyltransferase family. PRMT6 subfamily. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Methyltransferase; EC 2.1.1.125; Methyltransferase, protein arginine
Chromosomal Location of Human Ortholog: 1p13.3
Cellular Component: nucleoplasm; nucleus; cytosol
Molecular Function: histone methyltransferase activity; protein-arginine omega-N asymmetric methyltransferase activity; protein-arginine omega-N monomethyltransferase activity; protein binding; histone binding
Biological Process: histone methylation; establishment and/or maintenance of chromatin architecture; viral reproduction; transcription, DNA-dependent; base-excision repair; peptidyl-arginine methylation, to asymmetrical-dimethyl arginine; negative regulation of transcription, DNA-dependent
Research Articles on PRMT6
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Product Notes
The PRMT6 prmt6 (Catalog #AAA8100920) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PRMT6 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PRMT6 can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunofluorescence (IF). ICC/IF: 1:300-1:10,000. Researchers should empirically determine the suitability of the PRMT6 prmt6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PRMT6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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