PRMT6 recombinant protein

Recombinant PRMT6 protein

Cat. Num. AAA388114

• Gene Names: PRMT6; HRMT1L6
• Purity: This recombinant protein is >= 85% pure by SDS-PAGE.
• Synonyms: PRMT6; Recombinant PRMT6 protein; HRMT1L6; PRMT6 recombinant protein

• Host: Baculovirus
• Purity/Purification: This recombinant protein is >= 85% pure by SDS-PAGE.
• Form/Format: Full length recombinant PRMT6 protein was expressed in Sf9 cells and is supplied in 25mM Hepes pH7.5, 300mM NaCl, 5% glycerol, 0.04% Triton X-100, 0.2mM TCEP.

• Protein Species: Human
• Tag: DYKDDDDK-Tag
• Notes: Recombinant PRMT6 is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. HMT Assay Conditions:60 ng/ul Calf Thymus Octamers were incubated with 30 ng/ul recombinant PRMT6 protein in reaction buffer including 50mM Tris-HCl, pH 8.6, 0.02% Triton X-100, 2mM MgCl2, 1mM TCEP, 9 nCi/ul 3H-SAM for 3 hours at room temperature. Activity was detected by fluorography.
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.; Shipping Temp: Dry Ice

SDS-Page

(Recombinant PRMT6 protein gel. PRMT6 protein was run on a 10% SDS-PAGE gel and stained with Coomassie blue.)

Testing Data

(PRMT6 activity assay. 30 ng/ul Recombinant Histone H3.1 or 60 ng/ul Calf Thymus Octamer were incubated with 15 ng/ul PRMT6 (+) and 30 ng/ul PRMT6(++), respectively, in reaction buffer for 3 hours at room temperature. Activity was detected by fluorography.)

Related Product Information for PRMT6 recombinant protein

Short Description: Recombinant PRMT6 (accession number NP_060607.2) was expressed in Sf9 cells and contains an N-terminal DYKDDDDK tag with an observed molecular weight of 43.7 kDa. PRMT6 (Protein Arginine Methyltransferase 6, HRMT1L6, ANM6) is a type I arginine methyltransferase. Arginine methylation is a common post-translational modification of histones and other cellular proteins. PRMT6 preferentially methylates arginine residues present in GAR (glycine and arginine-rich) domains. PRMT6 asymmetrically dimethylates histone H3 at Arg2, which is mutually exclusive to methylation at lysine 4 (associated with transcriptional activation), and thus PRMT6 serves as a transcriptional repressor in this context. It can also symmetrically dimethylate H2A and H4 at Arg3 and serves as a coactivator of transcription in concert with nuclear receptors. Recombinant PRMT6 can be used to study enzyme kinetics and perform inhibitor screening assays.

Background: PRMT6 (Protein Arginine Methyltransferase 6, HRMT1L6, ANM6) is a type I arginine methyltransferase. Arginine methylation is a common post-translational modification of histones and other cellular proteins. PRMT6 preferentially methylates arginine residues present in GAR (glycine and arginine-rich) domains. PRMT6 asymmetrically dimethylates histone H3 at Arg2, which is mutually exclusive to methylation at lysine 4 (associated with transcriptional activation), and thus PRMT6 serves as a transcriptional repressor in this context. It can also symmetrically dimethylate H2A and H4 at Arg3 and serves as a coactivator of transcription in concert with nuclear receptors.

Product Categories/Family for PRMT6 recombinant protein

Recombinant Proteins; Histone Methyltransferase (HMT); Transcription

NCBI and Uniprot Product Information

• NCBI GI #: 227908867
• NCBI GeneID: 55170
• NCBI Accession #: NP_060607.2
• NCBI GenBank Nucleotide #: NM_018137.2
• UniProt Accession #: Q96LA8; Q5T5Y5; Q6DKI4; Q9NVR8; A3KME1; B4DID8
• Molecular Weight: 32,509 Da
• NCBI Official Full Name: protein arginine N-methyltransferase 6
• NCBI Official Synonym Full Names: protein arginine methyltransferase 6
• NCBI Official Symbol: PRMT6
• NCBI Official Synonym Symbols: HRMT1L6
• NCBI Protein Information: protein arginine N-methyltransferase 6; HMT1 hnRNP methyltransferase-like 6; histone-arginine N-methyltransferase PRMT6; heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 6
• UniProt Protein Name: Protein arginine N-methyltransferase 6
• Protein Family: Protein arginine N-methyltransferase
• UniProt Gene Name: PRMT6
• UniProt Synonym Gene Names: HRMT1L6
• UniProt Entry Name: ANM6_HUMAN

NCBI Description

The protein encoded by this gene belongs to the arginine N-methyltransferase family, which catalyze the sequential transfer of methyl group from S-adenosyl-L-methionine to the side chain nitrogens of arginine residues within proteins, to form methylated arginine derivatives and S-adenosyl-L-homocysteine. This protein can catalyze both, the formation of omega-N monomethylarginine and asymmetrical dimethylarginine, with a strong preference for the latter. It specifically mediates the asymmetric dimethylation of Arg2 of histone H3, and the methylated form represents a specific tag for epigenetic transcriptional repression. This protein also forms a complex with, and methylates DNA polymerase beta, resulting in stimulation of polymerase activity by enhancing DNA binding and processivity. [provided by RefSeq, Sep 2011]

Uniprot Description

PRMT6: Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and asymmetrical dimethylarginine (aDMA), with a strong preference for the formation of aDMA. Preferentially methylates arginyl residues present in a glycine and arginine-rich domain and displays preference for monomethylated substrates. Specifically mediates the asymmetric dimethylation of histone H3 'Arg-2' to form H3R2me2a. H3R2me2a represents a specific tag for epigenetic transcriptional repression and is mutually exclusive with methylation on histone H3 'Lys-4' (H3K4me2 and H3K4me3). It thereby acts as a transcription corepressor of various genes such as HOXA2. Also methylates histone H2A and H4 'Arg-3' (H2AR3me and H4R3me, respectively). Acts as a regulator of DNA base excision during DNA repair by mediating the methylation of DNA polymerase beta (POLB), leading to stimulate the polymerase activity by enhancing DNA binding and processivity. Methylates HMGA1. May play a role in innate immunity against HIV-1 in case of infection by methylating and impairing the function of various HIV-1 proteins such as Tat, Rev and Nucleocapsid protein p7 (NC). Belongs to the protein arginine N-methyltransferase family. PRMT6 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Methyltransferase; EC 2.1.1.125; Methyltransferase, protein arginine

Chromosomal Location of Human Ortholog: 1p13.3

Cellular Component: nucleoplasm; nucleus; cytosol

Molecular Function: histone methyltransferase activity; protein-arginine omega-N asymmetric methyltransferase activity; protein-arginine omega-N monomethyltransferase activity; protein binding; histone binding

Biological Process: histone methylation; establishment and/or maintenance of chromatin architecture; viral reproduction; transcription, DNA-dependent; base-excision repair; peptidyl-arginine methylation, to asymmetrical-dimethyl arginine; negative regulation of transcription, DNA-dependent