Rabbit PRMT6 Polyclonal Antibody | anti-PRMT6 antibody

PRMT6 antibody

Cat. Num. AAA840275

• Gene Names: PRMT6; HRMT1L6
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity purified
• Synonyms: PRMT6; Polyclonal Antibody; PRMT6 antibody; Protein arginine N-methyltransferase 6; Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 6; Histone-arginine N-methyltransferase; HRMT1L6; anti-PRMT6 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purified
• Form/Format: 100 ug (0.5 mg/ml) affinity purified rabbit anti-PRMT6 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 5 mM EDTA and 0.01% thimerosal.; Appearance: Colorless liquid
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 375

• Applicable Applications for anti-PRMT6 antibody: Western Blot (WB)
• Application Notes: Western Blot analysis (0.5-4 ug/ml). However, the optimal concentrations should be determined individually. Blocking peptide is available separately.
• Immunogen: Synthetic peptide surrounding amino acid 361 of rat PRMT6
• Positive Control: Jurkat cell lysate
• Handling: The antibody solution should be gently mixed before use.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for anti-PRMT6 antibody

Background: Protein arginine methylation is a form of post-translational modification that is catalyzed by protein arginine methyltransferases (PRMTs). PRMT6 catalyzes the sequential transfer of a methyl group from S-adenosyl-L-methionine to the side chain nitrogens of arginine residues within proteins to form methylated arginine derivatives and S-adenosyl-L-homocysteine. PRMT6 has been shown to act as a restriction factor for HIV replication.

Product Categories/Family for anti-PRMT6 antibody

Epigenetics; Antibodies and PeptidesAntibodies & Supporting Tools; Primary Antibodies (A-Z)

NCBI and Uniprot Product Information

• NCBI GI #: 227908867
• NCBI GeneID: 55170
• NCBI Accession #: NP_060607.2
• NCBI GenBank Nucleotide #: NM_018137.2
• UniProt Accession #: Q96LA8; Q5T5Y5; Q6DKI4; Q9NVR8; A3KME1; B4DID8
• Molecular Weight: 32,509 Da
• NCBI Official Full Name: protein arginine N-methyltransferase 6
• NCBI Official Synonym Full Names: protein arginine methyltransferase 6
• NCBI Official Symbol: PRMT6
• NCBI Official Synonym Symbols: HRMT1L6
• NCBI Protein Information: protein arginine N-methyltransferase 6
• UniProt Protein Name: Protein arginine N-methyltransferase 6
• Protein Family: Protein arginine N-methyltransferase
• UniProt Gene Name: PRMT6
• UniProt Synonym Gene Names: HRMT1L6
• UniProt Entry Name: ANM6_HUMAN

NCBI Description

The protein encoded by this gene belongs to the arginine N-methyltransferase family, which catalyze the sequential transfer of methyl group from S-adenosyl-L-methionine to the side chain nitrogens of arginine residues within proteins, to form methylated arginine derivatives and S-adenosyl-L-homocysteine. This protein can catalyze both, the formation of omega-N monomethylarginine and asymmetrical dimethylarginine, with a strong preference for the latter. It specifically mediates the asymmetric dimethylation of Arg2 of histone H3, and the methylated form represents a specific tag for epigenetic transcriptional repression. This protein also forms a complex with, and methylates DNA polymerase beta, resulting in stimulation of polymerase activity by enhancing DNA binding and processivity. [provided by RefSeq, Sep 2011]

Uniprot Description

PRMT6: Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and asymmetrical dimethylarginine (aDMA), with a strong preference for the formation of aDMA. Preferentially methylates arginyl residues present in a glycine and arginine-rich domain and displays preference for monomethylated substrates. Specifically mediates the asymmetric dimethylation of histone H3 'Arg-2' to form H3R2me2a. H3R2me2a represents a specific tag for epigenetic transcriptional repression and is mutually exclusive with methylation on histone H3 'Lys-4' (H3K4me2 and H3K4me3). It thereby acts as a transcription corepressor of various genes such as HOXA2. Also methylates histone H2A and H4 'Arg-3' (H2AR3me and H4R3me, respectively). Acts as a regulator of DNA base excision during DNA repair by mediating the methylation of DNA polymerase beta (POLB), leading to stimulate the polymerase activity by enhancing DNA binding and processivity. Methylates HMGA1. May play a role in innate immunity against HIV-1 in case of infection by methylating and impairing the function of various HIV-1 proteins such as Tat, Rev and Nucleocapsid protein p7 (NC). Belongs to the protein arginine N-methyltransferase family. PRMT6 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Methyltransferase; Methyltransferase, protein arginine; EC 2.1.1.125

Chromosomal Location of Human Ortholog: 1p13.3

Cellular Component: nucleoplasm; nucleus; cytosol

Molecular Function: protein-arginine omega-N monomethyltransferase activity; histone methyltransferase activity; protein-arginine omega-N asymmetric methyltransferase activity; protein binding; histone binding

Biological Process: histone methylation; establishment and/or maintenance of chromatin architecture; transcription, DNA-dependent; viral reproduction; base-excision repair; peptidyl-arginine methylation, to asymmetrical-dimethyl arginine; negative regulation of transcription, DNA-dependent