Rabbit anti-Human PRKRA Polyclonal Antibody | anti-PRKRA antibody

PRKRA, phosphorylated (Ser246) (Interferon-inducible Double Stranded RNA-dependent Protein Kinase Activator A, Protein Kinase, Interferon-inducible Double Stranded RNA-dependent Activator, Protein Activator Of The Interferon-induced Protein Kinase, PKR-as

Cat. Num. AAA628726

• Reactivity: Human
• Applications: ELISA, Gel Super Shift Assay
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: PRKRA; Polyclonal Antibody; phosphorylated (Ser246) (Interferon-inducible Double Stranded RNA-dependent Protein Kinase Activator A; Protein Kinase; Interferon-inducible Double Stranded RNA-dependent Activator; Protein Activator Of The Interferon-induced Protein Kinase; PKR-as; Anti -PRKRA; anti-PRKRA antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PRKRA when phosphorylated at Ser246.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-PRKRA antibody: ELISA (EL/EIA), Gel Shift Assay (GS/EMSA)
• Application Notes: Suitable for use in ELISA and Dot Blot.; Dilution: ELISA: 1:1,000; Dot Blot: 1:500
• Immunogen: Synthetic phosphopeptide corresponding to amino acid residues surrounding Ser246 of human PRKRA (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Dot Blot (DB)

(Dot blot analysis of anti-Phospho-PRKRA-pS246 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)

Related Product Information for anti-PRKRA antibody

PRKRA appears to have a pro-apoptotic function that may be suppressed in the presence of growth factor. It activates EIF2AK2 in the absence of double-stranded RNA (dsRNA).

Product Categories/Family for anti-PRKRA antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 49065476
• UniProt Accession #: O75569; Q53G24; Q6X7T5; Q8NDK4; A8K3I6
• Molecular Weight: 34,404 Da
• NCBI Official Full Name: PRKRA
• UniProt Protein Name: Interferon-inducible double stranded RNA-dependent protein kinase activator A
• Protein Family: Interferon-inducible double-stranded RNA-dependent protein kinase activator
• UniProt Gene Name: PRKRA
• UniProt Synonym Gene Names: PACT; RAX
• UniProt Entry Name: PRKRA_HUMAN

NCBI Description

This gene encodes a protein kinase activated by double-stranded RNA which mediates the effects of interferon in response to viral infection. Mutations in this gene have been associated with dystonia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2008]

Uniprot Description

PACT: a double strand RNA binding protein (dsRBP). Interacts with TRBP and Dicer to stimulate the cleavage of double-stranded or short hairpin RNA to siRNA. Appears to have a pro-apoptotic function that may be suppressed in the presence of growth factor. Activates the interferon-induced protein kinase PKR. Three alternatively spliced human isoforms have been reported.

Protein type: RNA processing; Activator; RNA-binding

Chromosomal Location of Human Ortholog: 2q31.2

Cellular Component: nucleoplasm; membrane; perinuclear region of cytoplasm; cytoplasm; cytosol

Molecular Function: protein binding; protein homodimerization activity; enzyme activator activity

Biological Process: positive regulation of catalytic activity; negative regulation of cell proliferation; skeletal morphogenesis; response to virus; outer ear morphogenesis; gene expression; immune response; middle ear morphogenesis; RNA interference, production of siRNA; protein amino acid phosphorylation

Disease: Dystonia 16