Rabbit anti-Human PRKRA Polyclonal Antibody | anti-PRKRA antibody

PRKRA (Interferon-inducible Double Stranded RNA-dependent Protein Kinase Activator A, PKR-associated Protein X, PACT, RAX, PKR-associating Protein X, Protein Activator of the Interferon-induced Protein Kinase, Protein Kinase, Interferon-inducible Double S

Cat. Num. AAA6390784

• Gene Names: PRKRA; RAX; PACT; DYT16; HSD14
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PRKRA; Polyclonal Antibody; PRKRA (Interferon-inducible Double Stranded RNA-dependent Protein Kinase Activator A; PKR-associated Protein X; PACT; RAX; PKR-associating Protein X; Protein Activator of the Interferon-induced Protein Kinase; Protein Kinase; Interferon-inducible Double S; anti-PRKRA antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PRKRA.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-PRKRA antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human PRKRA, aa1-313 (NP_003681.1).
• Immunogen Sequence: MSQSRHRAEAPPLEREDSGTFSLGKMITAKPGKTPIQVLHEYGMKTKNIPVYECERSDVQIHVPTFTFRVTVGDITCTGEGTSKKLAKHRAAEAAINILKANASICFAVPDPLMPDPSKQPKNQLNPIGSLQELAIHHGWRLPEYTLSQEGGPAHKREYTTICRLESFMETGKGASKKQAKRNAAEKFLAKFSNISPENHISLTNVVGHSLGCTWHSLRNSPGEKINLLKRSLLSIPNTDYIQLLSEIAKEQGFNITYLDIDELSANGQYQCLAELSTSPITVCHGSGISCGNAQSDAAHNALQYLKIIAERK
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of PRKRA expression in transfected 293T cell line by PRKRA polyclonal antibody. Lane 1: PRKRA transfected lysate (34.4kD). Lane 2: Non-transfected lysate.)

Western Blot (WB)

(PRKRA rabbit polyclonal antibody. Western Blot analysis of PRKRA expression in human liver.)

Related Product Information for anti-PRKRA antibody

PRKRA appears to have a pro-apoptotic function that may be suppressed in the presence of growth factor. It activates EIF2AK2 in the absence of double-stranded RNA (dsRNA).

Product Categories/Family for anti-PRKRA antibody

Antibodies; Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 4505581
• NCBI GeneID: 8575
• NCBI Accession #: NP_003681.1
• NCBI GenBank Nucleotide #: NM_003690
• UniProt Accession #: O75569; Q53G24; Q6X7T5; Q8NDK4; A8K3I6
• Molecular Weight: 31,635 Da
• NCBI Official Full Name: interferon-inducible double-stranded RNA-dependent protein kinase activator A isoform 1
• NCBI Official Synonym Full Names: protein kinase, interferon-inducible double stranded RNA dependent activator
• NCBI Official Symbol: PRKRA
• NCBI Official Synonym Symbols: RAX; PACT; DYT16; HSD14
• NCBI Protein Information: interferon-inducible double-stranded RNA-dependent protein kinase activator A; PKR-associated protein X; PKR-associating protein X; protein activator of the interferon-induced protein kinase; protein kinase, interferon-inducible double stranded RNA-depend
• UniProt Protein Name: Interferon-inducible double-stranded RNA-dependent protein kinase activator A
• Protein Family: Interferon-inducible double-stranded RNA-dependent protein kinase activator
• UniProt Gene Name: PRKRA
• UniProt Synonym Gene Names: PACT; RAX
• UniProt Entry Name: PRKRA_HUMAN

NCBI Description

This gene encodes a protein kinase activated by double-stranded RNA which mediates the effects of interferon in response to viral infection. Mutations in this gene have been associated with dystonia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2008]

Uniprot Description

PACT: a double strand RNA binding protein (dsRBP). Interacts with TRBP and Dicer to stimulate the cleavage of double-stranded or short hairpin RNA to siRNA. Appears to have a pro-apoptotic function that may be suppressed in the presence of growth factor. Activates the interferon-induced protein kinase PKR. Three alternatively spliced human isoforms have been reported.

Protein type: Activator; RNA processing; RNA-binding

Chromosomal Location of Human Ortholog: 2q31.2

Cellular Component: cytoplasm; cytosol; membrane; nucleoplasm

Molecular Function: double-stranded RNA binding; enzyme binding; identical protein binding; protein binding; protein homodimerization activity

Biological Process: immune response; miRNA-mediated gene silencing, production of miRNAs; negative regulation of cell proliferation; pre-microRNA processing; response to virus; RNA interference, production of siRNA; RNA-mediated gene silencing

Disease: Dystonia 16