Rabbit anti-Human PRKRA Polyclonal Antibody | anti-PRKRA antibody

PRKRA (S246) (Interferon-inducible Double Stranded RNA-dependent Protein Kinase Activator A, Protein Kinase, Interferon-inducible Double Stranded RNA-dependent Activator, Protein Activator Of The Interferon-induced Protein Kinase, PKR-associated Protein X

Cat. Num. AAA630885

• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: PRKRA; Polyclonal Antibody; PRKRA (S246) (Interferon-inducible Double Stranded RNA-dependent Protein Kinase Activator A; Protein Kinase; Interferon-inducible Double Stranded RNA-dependent Activator; Protein Activator Of The Interferon-induced Protein Kinase; PKR-associated Protein X; Anti -PRKRA (S246) (Interferon-inducible Double Stranded RNA-dependent Protein Kinase Activator A; anti-PRKRA antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PRKRA.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-PRKRA antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot, and Immunohistochemistry.; Dilution: ELISA: 1:1,000; Western Blot: 1:50-1:100; Immunohistochemistry: 1:50-1:100
• Immunogen: Synthetic peptide selected from S246 region of human PRKRA (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-PRKRA Antibody (S246) in HL60 cell line lysates (35ug/lane). PRKRA (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human placenta reacted with PRKRA Antibody (S246), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(PRKRA Antibody (S246) flow cytometric analysis of HL60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-PRKRA antibody

PRKRA appears to have a pro-apoptotic function that may be suppressed in the presence of growth factor. It activates EIF2AK2 in the absence of double-stranded RNA (dsRNA).

Product Categories/Family for anti-PRKRA antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 49065476
• UniProt Accession #: O75569; Q53G24; Q6X7T5; Q8NDK4; A8K3I6
• Molecular Weight: 34,404 Da
• NCBI Official Full Name: PRKRA
• UniProt Protein Name: Interferon-inducible double stranded RNA-dependent protein kinase activator A
• Protein Family: Interferon-inducible double-stranded RNA-dependent protein kinase activator
• UniProt Gene Name: PRKRA
• UniProt Synonym Gene Names: PACT; RAX
• UniProt Entry Name: PRKRA_HUMAN

NCBI Description

This gene encodes a protein kinase activated by double-stranded RNA which mediates the effects of interferon in response to viral infection. Mutations in this gene have been associated with dystonia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2008]

Uniprot Description

PACT: a double strand RNA binding protein (dsRBP). Interacts with TRBP and Dicer to stimulate the cleavage of double-stranded or short hairpin RNA to siRNA. Appears to have a pro-apoptotic function that may be suppressed in the presence of growth factor. Activates the interferon-induced protein kinase PKR. Three alternatively spliced human isoforms have been reported.

Protein type: RNA processing; RNA-binding; Activator

Chromosomal Location of Human Ortholog: 2q31.2

Cellular Component: nucleoplasm; membrane; perinuclear region of cytoplasm; cytoplasm; cytosol

Molecular Function: protein binding; protein homodimerization activity; enzyme activator activity

Biological Process: positive regulation of catalytic activity; negative regulation of cell proliferation; skeletal morphogenesis; response to virus; outer ear morphogenesis; gene expression; immune response; middle ear morphogenesis; RNA interference, production of siRNA; protein amino acid phosphorylation

Disease: Dystonia 16