Western Blot (WB)
(PPAN Antibody (C-term) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the PPAN antibody detected the PPAN protein (arrow).)
Rabbit anti-Human PPAN Polyclonal Antibody | anti-PPAN antibody
PPAN Antibody (C-term)
Reactivity
Human
Applications
Western Blot, ELISA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
PPAN; Polyclonal Antibody; PPAN Antibody (C-term); Suppressor of SWI4 1 homolog; Ssf-1; Brix domain-containing protein 3; Peter Pan homolog; BXDC3; SSF1; anti-PPAN antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This PPAN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 422-450 amino acids from the C-terminal region of human PPAN.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.48 (varies by lot)
Sequence Positions
422-450
Sequence Length
520
Applicable Applications for anti-PPAN antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(PPAN Antibody (C-term) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the PPAN antibody detected the PPAN protein (arrow).)
Western Blot (WB)
(PPAN Antibody (C-term) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the PPAN antibody detected the PPAN protein (arrow).)
Related Product Information for anti-PPAN antibody
The protein encoded by this gene is an evolutionarily
conserved protein similar to yeast SSF1 as well as to the gene
product of the Drosophila gene peter pan (ppan). SSF1 is known to
be involved in the second step of mRNA splicing. Both SSF1 and ppan
are essential for cell growth and proliferation. Exogenous
expression of this gene was reported to reduce the
anchorage-independent growth of some tumor cells. Read-through
transcription of this gene with P2RY11/P2Y(11), an adjacent
downstream gene that encodes an ATP receptor, has been found. These
read-through transcripts are ubiquitously present and up-regulated
during granulocyte differentiation.
conserved protein similar to yeast SSF1 as well as to the gene
product of the Drosophila gene peter pan (ppan). SSF1 is known to
be involved in the second step of mRNA splicing. Both SSF1 and ppan
are essential for cell growth and proliferation. Exogenous
expression of this gene was reported to reduce the
anchorage-independent growth of some tumor cells. Read-through
transcription of this gene with P2RY11/P2Y(11), an adjacent
downstream gene that encodes an ATP receptor, has been found. These
read-through transcripts are ubiquitously present and up-regulated
during granulocyte differentiation.
References
Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Nousiainen, M., et al. Proc. Natl. Acad. Sci. U.S.A. 103(14):5391-5396(2006)
Beausoleil, S.A., et al. Proc. Natl. Acad. Sci. U.S.A. 101(33):12130-12135(2004)
Duhant, X., et al. J. Immunol. 169(1):15-21(2002)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Nousiainen, M., et al. Proc. Natl. Acad. Sci. U.S.A. 103(14):5391-5396(2006)
Beausoleil, S.A., et al. Proc. Natl. Acad. Sci. U.S.A. 101(33):12130-12135(2004)
Duhant, X., et al. J. Immunol. 169(1):15-21(2002)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
53194
NCBI Official Full Name
PPAN-P2RY11 protein isoform 1
NCBI Official Synonym Full Names
PPAN-P2RY11 readthrough
NCBI Official Symbol
PPAN-P2RY11
NCBI Protein Information
PPAN-P2RY11 protein
UniProt Protein Name
Suppressor of SWI4 1 homolog
Protein Family
UniProt Gene Name
PPAN
UniProt Synonym Gene Names
BXDC3; SSF1; Ssf-1
UniProt Entry Name
SSF1_HUMAN
NCBI Description
This locus represents naturally occurring read-through transcription between the adjacent PPAN and P2RY11 genes. Alternative splicing results in two transcript variants, one of which encodes a fusion protein that shares sequence identity with each individual gene product. This transcript is found to be ubiquitously expressed and is up-regulated by agents inducing granulocytic differentiation. However, its functional significance in vivo remains unclear. [provided by RefSeq, Nov 2010]
Uniprot Description
SSF1: May have a role in cell growth. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: RNA-binding; Nucleolus
Chromosomal Location of Human Ortholog: 19p13
Cellular Component: nucleolus; nucleus
Biological Process: RNA splicing
Similar Products
Product Notes
The PPAN ppan (Catalog #AAA9208165) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 422-450. The PPAN Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PPAN can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the PPAN ppan for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PPAN, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
