Loading...

Skip to main content

Call us on + 1 (800) 604-9114 for more information about our products

Looking for specific datasheet Manual/COA/MSDS?
Request a Manual/COA/MSDS

Interested to get a quote about our products?
Request a Quote

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human stomach cancer tissue slide using MBS7602111 (NT5E,CD73 Antibody) at dilution of 1:200)

Rabbit NT5E/CD73 Polyclonal Antibody | anti-NT5E/CD73 antibody

NT5E/CD73 Rabbit Polyclonal

Gene Names
NT5E; NT; eN; NT5; NTE; eNT; CD73; E5NT; CALJA
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Purity: > or =95% as determined by SDS-PAGE
Purification: Immunogen Affinity Purified
Synonyms
NT5E/CD73; Polyclonal Antibody; NT5E/CD73 Rabbit Polyclonal; 5 NT; 5 nucleotidase; 5' nucleotidase; ecto (CD73); CD73; E5NT; Ecto 5 nucleotidase; eN; eNT; NT; NT5; NT5E; NTE; anti-NT5E/CD73 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Human, Mouse, Rat; other species are not tested. Please decide the specificity by homology
Purity/Purification
Purity: > or =95% as determined by SDS-PAGE
Purification: Immunogen Affinity Purified
Form/Format
Liquid
Sequence Length
264
Applicable Applications for anti-NT5E/CD73 antibody
ELISA (EIA), Western Blot (WB), Immunohistichemistry (IHC)
Application Notes
WB: 1:500-1:5000
IHC: 1:50-1:500
Immunogen
5'-nucleotidase, ecto (CD73)
Buffer
PBS with 0.02% sodium azide and 50% glycerol pH 7.3
Preparation and Storage
Store at -20°C for 24 months (Avoid repeated freeze / thaw cycles.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human stomach cancer tissue slide using MBS7602111 (NT5E,CD73 Antibody) at dilution of 1:200)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human stomach cancer tissue slide using MBS7602111 (NT5E,CD73 Antibody) at dilution of 1:200)

Western Blot (WB)

(Jurkat cells were subjected to SDS PAGE followed by western blot with MBS7602111 (NT5E,CD73 antibody) at dilution of 1:1000)

Western Blot (WB) (Jurkat cells were subjected to SDS PAGE followed by western blot with MBS7602111 (NT5E,CD73 antibody) at dilution of 1:1000)
Related Product Information for anti-NT5E/CD73 antibody
NT5E (5'-nucleotidase) is also named as NT5, NTE, CD73, ecto-5'-nucleotidase, and belongs to the 5'-nucleotidase family. It catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP.The enzyme occurs mainly as a dimer and the wide distribution of NT5E in the rat hippocampus, suggesting their involvement in the control of the purinergic signaling(PMID:17619139). This protein has two isoforms with the molecular weight of 63 kDa and 58 kDa and four glycosylation sites with a signalpeptide. It can exsit as a dimer (PMID:17619139). It can be detected the band of 55 kDa, 64 kDa, 70 kDa, 110 kDa by western blot (PMID:17619139; 12370277; 21533188). Defects in NT5E are the cause of calcification of joints and arteries (CALJA).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
Molecular Weight
63kDa
NCBI Official Full Name
NT5E protein
NCBI Official Synonym Full Names
5'-nucleotidase ecto
NCBI Official Symbol
NT5E
NCBI Official Synonym Symbols
NT; eN; NT5; NTE; eNT; CD73; E5NT; CALJA
NCBI Protein Information
5'-nucleotidase
UniProt Protein Name
5'-nucleotidase
UniProt Gene Name
NT5E
UniProt Synonym Gene Names
NT5; NTE; 5'-NT
UniProt Entry Name
5NTD_HUMAN

NCBI Description

The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011]

Uniprot Description

NT5E: Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Homodimer; disulfide-linked. Belongs to the 5'-nucleotidase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nucleotide Metabolism - pyrimidine; Phosphatase (non-protein); Nucleotide Metabolism - purine; Membrane protein, GPI anchor; EC 3.1.3.5; Membrane protein, integral; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide

Chromosomal Location of Human Ortholog: 6q14-q21

Cellular Component: cell surface; cytoplasm; membrane; plasma membrane

Molecular Function: 5'-nucleotidase activity; ferrous iron binding; nucleotide binding

Biological Process: adenosine biosynthetic process; AMP catabolic process; brain development; dephosphorylation; DNA metabolic process; leukocyte adhesion; negative regulation of inflammatory response; positive regulation of lipid biosynthetic process; purine nucleotide biosynthetic process; purine nucleotide catabolic process; pyrimidine nucleoside catabolic process; response to aluminum ion

Disease: Calcification Of Joints And Arteries

Research Articles on NT5E/CD73

Similar Products

Product Notes

The NT5E/CD73 nt5e (Catalog #AAA7602111) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NT5E/CD73 Rabbit Polyclonal reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's NT5E/CD73 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistichemistry (IHC). WB: 1:500-1:5000 IHC: 1:50-1:500. Researchers should empirically determine the suitability of the NT5E/CD73 nt5e for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NT5E/CD73, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

Item has been added to Shopping Cart

If you are ready to order, navigate to Shopping Cart and get ready to checkout.

Looking for a specific manual?
Request a Manual

Request more Information

Please complete the form below and a representative will contact you as soon as possible.

Request a Manual

Please complete the form below and a representative will contact you as soon as possible.

Request a Quote

Please complete the form below and a representative will contact you as soon as possible.