Rabbit anti-Human PPAN Polyclonal Antibody | anti-PPAN antibody

PPAN, CT (PPAN, BXDC3, SSF1, Suppressor of SWI4 1 homolog, Brix domain-containing protein 3, Peter Pan homolog) (APC)

Cat. Num. AAA6336083

• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PPAN; Polyclonal Antibody; CT (PPAN; BXDC3; SSF1; Suppressor of SWI4 1 homolog; Brix domain-containing protein 3; Peter Pan homolog) (APC); Peter Pan homolog; anti-PPAN antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-PPAN antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: PPAN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 421-450 amino acids from the C-terminal region of human PPAN.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(PPAN Antibody (C-term) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the PPAN antibody detected the PPAN protein (arrow).)

Related Product Information for anti-PPAN antibody

The protein encoded by this gene is an evolutionarily conserved protein similar to yeast SSF1 as well as to the gene product of the Drosophila gene peter pan (ppan). SSF1 is known to be involved in the second step of mRNA splicing. Both SSF1 and ppan are essential for cell growth and proliferation. Exogenous expression of this gene was reported to reduce the anchorage-independent growth of some tumor cells. Read-through transcription of this gene with P2RY11/P2Y(11), an adjacent downstream gene that encodes an ATP receptor, has been found. These read-through transcripts are ubiquitously present and up-regulated during granulocyte differentiation.

Product Categories/Family for anti-PPAN antibody

Antibodies; Transcription Factors

References

Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007). Olsen, J.V., et al. Cell 127(3):635-648(2006). Nousiainen, M., et al. Proc. Natl. Acad. Sci. U.S.A. 103(14):5391-5396(2006). Beausoleil, S.A., et al. Proc. Natl. Acad. Sci. U.S.A. 101(33):12130-12135(2004). Duhant, X., et al. J. Immunol. 169(1):15-21(2002).

NCBI and Uniprot Product Information

• NCBI GI #: 99028871
• NCBI GeneID: 692312
• NCBI Accession #: NP_001035754.1; NP_064615.3
• NCBI GenBank Nucleotide #: NM_001040664.2
• UniProt Accession #: Q9NQ55; Q9BW97; Q9H170; C9J3F9
• Molecular Weight: 58,199 Da
• NCBI Official Full Name: PPAN-P2RY11 protein isoform 1
• NCBI Official Synonym Full Names: PPAN-P2RY11 readthrough
• NCBI Official Symbol: PPAN-P2RY11
• NCBI Protein Information: PPAN-P2RY11 protein
• UniProt Protein Name: Suppressor of SWI4 1 homolog
• Protein Family: Protein
• UniProt Gene Name: PPAN
• UniProt Synonym Gene Names: BXDC3; SSF1; Ssf-1
• UniProt Entry Name: SSF1_HUMAN

NCBI Description

This locus represents naturally occurring read-through transcription between the adjacent PPAN and P2RY11 genes. Alternative splicing results in two transcript variants, one of which encodes a fusion protein that shares sequence identity with each individual gene product. This transcript is found to be ubiquitously expressed and is up-regulated by agents inducing granulocytic differentiation. However, its functional significance in vivo remains unclear. [provided by RefSeq, Nov 2010]

Uniprot Description

SSF1: May have a role in cell growth. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding; Nucleolus

Chromosomal Location of Human Ortholog: 19p13

Cellular Component: nucleolus; nucleus

Biological Process: RNA splicing