Western Blot (WB)
(Western Blot analysis of Rat brain membrane lysate showing detection of KCC2 protein using Mouse Anti-KCC2 Monoclonal Antibody, Clone S1-12. Primary Antibody: Mouse Anti-KCC2 Monoclonal Antibody at 1:1000.)
Mouse KCC2 Monoclonal Antibody | anti-Slc12a5 antibody
KCC2 Antibody: Alkaline Phosphatase
Western Blot (WB)
(Western Blot analysis of Rat brain membrane lysate showing detection of KCC2 protein using Mouse Anti-KCC2 Monoclonal Antibody, Clone S1-12. Primary Antibody: Mouse Anti-KCC2 Monoclonal Antibody at 1:1000.)
Western Blot (WB)
(Western Blot analysis of Rat brain membrane lysate showing detection of KCC2 protein using Mouse Anti-KCC2 Monoclonal Antibody, Clone S1-12. Primary Antibody: Mouse Anti-KCC2 Monoclonal Antibody at 1:1000.)
Scientific Background: KCC2 is a member of the cation-chloride cotransporter gene family (1). It acts as a K-Cl cotransporter. KCCs normally lower intracellular chloride concentrations below the electrochemical equilibrium potential and depending on the chemical concentration gradients of potassium and chloride, KCC2 can operate as a net efflux or influx pathway. It is proposed to act as the main chloride extruder to promote fast hyperpolarizing postsynaptic inhibition in the brain (2, 3). KCC2 is expressed at high levels in neurons throughout the nervous system and immunofluorescence shows that the protein is localized at inhibitory synapses of the spinal cord (4). Studies in mice have shown that KCC2 reduces GABA's inhibitory signaling, resulting in motor defects, epilepsy, and anxiety-like behavior.
NCBI and Uniprot Product Information
NCBI Description
neuronal specific potassium-chloride cotransporter isoform; may play a role in response to neuronal injury [RGD, Feb 2006]
Uniprot Description
KCC2: Mediates electroneutral potassium-chloride cotransport in mature neurons. Transport occurs under isotonic conditions, but is activated 20-fold by cell swelling. Important for Cl(-) homeostasis in neurons. Homomultimer and heteromultimer with other K-Cl cotransporters. Interacts with AP2A1. Brain specific. Detected in neuronal cells. Inhibited by WNK3. Belongs to the SLC12A transporter family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, multi-pass; Membrane protein, integral; Transporter; Transporter, SLC family
Cellular Component: cell soma; integral to plasma membrane; plasma membrane; perikaryon; dendritic shaft
Molecular Function: protein binding; ammonium transmembrane transporter activity; protein kinase binding; potassium:chloride symporter activity
Biological Process: response to drug; synaptic transmission; multicellular organism growth; intracellular pH reduction; chloride transport; learning; potassium ion transport; ammonium transport; thermosensory behavior
Research Articles on Slc12a5
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Product Notes
The Slc12a5 slc12a5 (Catalog #AAA801868) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The KCC2 Antibody: Alkaline Phosphatase reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's KCC2 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Western Blot (WB), Immunoprecipitation (IP). 1:300 (IHC). Researchers should empirically determine the suitability of the Slc12a5 slc12a5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KCC2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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