Human IL-17RB ELISA Kit | IL17RB elisa kit

Human IL-17RB PicoKine ELISA Kit

Cat. Num. AAA177128

• Gene Names: IL17RB; CRL4; EVI27; IL17BR; IL17RH1
• Reactivity: Human
• Synonyms: IL-17RB; Human IL-17RB PicoKine ELISA Kit; Interleukin-17 receptor B; interleukin 17 receptor B; CRL 4; CRL4; Cytokine receptor CRL4; EVI 27; EVI27; IL 17 receptor B; IL 17 receptor homolog 1; IL 17B receptor; IL 17RB; IL 17Rh1; IL17BR; IL17RB; IL17RH1; Interleukin 17 receptor B; Interleukin 17 receptor homolog 1; Interleukin 17 receptor homolog; Interleukin 17B receptor; MGC5245; IL17RB elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant human IL-17RB
• Sequence Length: 502

• Samples: Cell culture supernates, serum and plasma (heparin, EDTA)
• Assay Type: Sandwich
• Detection Range: 156pg/ml-10,000pg/ml
• Sensitivity: < 10pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

(Human IL-17RB PicoKine ELISA Kit standard curve )

Related Product Information for IL17RB elisa kit

Principle of the assay: human IL-17RB ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-17RB has been precoated onto 96-well plates. Standards(NSO, R18-K286) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-17RB is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-17RB amount of sample captured in plate.
Background: IL17RB, also known as CRL4, is a protein that in humans is encoded by the IL17RB gene. It is located on 3p21.1. IL17RB is produced by activated T cells exhibiting proinflammatory activities. The protein encoded by this gene is a cytokine receptor. This receptor specifically binds to IL17B and IL17E (IL25), but does not bind to IL17 (A) or IL17C. This receptor has been shown to mediate the activation of NF-kappaB and the production of IL8 induced by IL17E. And it may play a role in controlling the growth and/or differentiation of hematopoietic cells.

NCBI and Uniprot Product Information

• NCBI GI #: 21263748
• NCBI GeneID: 55540
• UniProt Accession #: Q9NRM6; Q9BPZ0; Q9NRL4; Q9NRM5
• Molecular Weight: 31,773 Da
• NCBI Official Full Name: Interleukin-17 receptor B
• NCBI Official Synonym Full Names: interleukin 17 receptor B
• NCBI Official Symbol: IL17RB
• NCBI Official Synonym Symbols: CRL4; EVI27; IL17BR; IL17RH1
• NCBI Protein Information: interleukin-17 receptor B; IL-17RB; IL-17Rh1; IL-17B receptor; IL-17 receptor B; cytokine receptor CRL4; IL-17 receptor homolog 1; cytokine receptor-like 4; interleukin 17B receptor; interleukin-17B receptor; interleukin 17 receptor homolog 1
• UniProt Protein Name: Interleukin-17 receptor B
• Protein Family: Interleukin-17 receptor
• UniProt Gene Name: IL17RB
• UniProt Synonym Gene Names: CRL4; EVI27; IL17BR; IL-17 receptor B; IL-17RB; IL-17Rh1; IL17Rh1; IL-17B receptor
• UniProt Entry Name: I17RB_HUMAN

NCBI Description

The protein encoded by this gene is a cytokine receptor. This receptor specifically binds to IL17B and IL17E, but does not bind to IL17 and IL17C. This receptor has been shown to mediate the activation of NF-kappaB and the production of IL8 induced by IL17E. The expression of the rat counterpart of this gene was found to be significantly up-regulated during intestinal inflammation, which suggested the immunoregulatory activity of this receptor. [provided by RefSeq, Jul 2008]

Uniprot Description

IL17RB: Receptor for the proinflammatory cytokines IL17B and IL17E. May play a role in controlling the growth and/or differentiation of hematopoietic cells. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 3p21.1

Cellular Component: cell surface; membrane; integral to plasma membrane; cytoplasm; extracellular region

Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor activity; interleukin-17 receptor activity

Biological Process: cytokine and chemokine mediated signaling pathway; defense response; regulation of cell growth