Goat anti-Human Plexin D1 Polyclonal Antibody | anti-PLXND1 antibody

Plexin D1 (Biotin)

Cat. Num. AAA616656

• Gene Names: PLXND1; PLEXD1; KIAA0620; MGC75353
• Reactivity: Human
• Applications: Western Blot, Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by human Plexin D1 affinity chromatography.
• Synonyms: Plexin D1; Polyclonal Antibody; Plexin D1 (Biotin); Anti -Plexin D1 (Biotin); anti-PLXND1 antibody

• Host: Goat
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Plexin D1 extracellular domain. Species sequence homology: Mouse 89% and canine 91%.
• Purity/Purification: Affinity Purified; Purified by human Plexin D1 affinity chromatography.
• Form/Format: Supplied as a lyophilized powder from PBS with BSA. Labeled with biotin. Reconstitute with 1ml TBS, pH7.3, containing 0.1% BSA to a concentration of 0.05mg/ml.

• Applicable Applications for anti-PLXND1 antibody: Western Blot (WB), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Western Blot and Flow cytometry.; Dilution: Western blot: 0.1 0.2ug/ml. The detection limit is~1ng/lane under non-reducing and reducing conditions.; Flow cytometry: 25ug/ml. Add 10il of the diluted solution to 1-2.5 x 10e5 cells in a total reaction volume not exceeding 200ul. The binding of biotinylated antibodies may be visualized by adding a secondary developing reagent such as streptavidin conjugated to a fluorochrome.
• Immunogen: NS0-derived recombinant human rhPlexin D1 extracellular domain.
• Hybridoma: Produced in a goat immunized with purified, NS0-derived, recombinant human Plexin D1 extracellular domain (rhPlexin D1; aa 47-1271).
• Preparation and Storage: Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-PLXND1 antibody

Plexin D1 is a type I transmembrane protein that is the prototype of the Plexin D subfamily in the Plexin family. It is expressed on multiple tissues including fetal neuronal and endothelial cells. Plexin D1 expression is also induced on tumor vasculature and tumor cells. The 1879 amino acid (aa) mature human Plexin D1 contains a large (1225 aa) extracellular domain with a 500 aa sema domain and three short MRS (Met-related sequences) cysteine-rich motifs. By alternative splicing, multiple Plexin-D1 isoforms may exist. Plexin D1 binds Sema4A to suppress

Product Categories/Family for anti-PLXND1 antibody

Antibodies; Abs to Plexin; Semaphorin

NCBI and Uniprot Product Information

• NCBI GI #: 157694524
• NCBI GeneID: 23129
• NCBI Accession #: NP_055918.2
• NCBI GenBank Nucleotide #: NM_015103.2
• UniProt Accession #: Q9Y4D7; Q6PJS9; Q8IZJ2; Q9BTQ2; A7E2C6; C9JPZ6; Q6P657; Q86YC4
• Molecular Weight: 212,007 Da
• NCBI Official Full Name: plexin-D1
• NCBI Official Synonym Full Names: plexin D1
• NCBI Official Symbol: PLXND1
• NCBI Official Synonym Symbols: PLEXD1; KIAA0620; MGC75353
• NCBI Protein Information: plexin-D1; OTTHUMP00000215785
• UniProt Protein Name: Plexin-D1
• Protein Family: Plexin
• UniProt Gene Name: PLXND1
• UniProt Synonym Gene Names: KIAA0620
• UniProt Entry Name: PLXD1_HUMAN

Uniprot Description

PLXND1: Cell surface receptor for SEMA4A and for class 3 semaphorins, such as SEMA3A, SEMA3C and SEMA3E. Plays an important role in cell-cell signaling, and in regulating the migration of a wide spectrum of cell types. Regulates the migration of thymocytes in the medulla. Regulates endothelial cell migration. Plays an important role in ensuring the specificity of synapse formation. Required for normal development of the heart and vasculature. Mediates anti-angiogenic signaling in response to SEMA3E. Belongs to the plexin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 3q22.1

Cellular Component: integral to plasma membrane; lamellipodium; plasma membrane; intracellular

Molecular Function: protein domain specific binding; protein binding; semaphorin receptor activity

Biological Process: patterning of blood vessels; axon guidance; synaptogenesis; positive regulation of protein binding; endothelial cell migration; angiogenesis; regulation of cell migration; regulation of angiogenesis