Rabbit Plexin D1 Polyclonal Antibody | anti-PLXND1 antibody

Plexin D1 (Sema Domain)

Cat. Num. AAA474129

• Gene Names: PLXND1; PLEXD1
• Reactivity: Human, Rat, Mouse
• Applications: Western Blot, ELISA
• Purity: Affinity Purified
• Synonyms: Plexin D1; Polyclonal Antibody; Plexin D1 (Sema Domain); PLXND1; Sema3E; anti-PLXND1 antibody

• Host: Rabbit
• Reactivity: Human, Rat, Mouse
• Clonality: Polyclonal
• Specificity: This antibody was affinity purified using Plexin D1 (Sema Domain) peptide (without carrier). The antibody detects 250, 215, and 195 kDa bands corresponding to the apparent molecular mass of Plexin D1 variants in human umbilical vein endothelial cells.
• Purity/Purification: Affinity Purified
• Form/Format: Supplied in 100ul phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.
• Sequence Length: 52

• Applicable Applications for anti-PLXND1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA: 1:2000; WB: 1:500; WB: Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1 hour at room temperature.
• Immunogen: Plexin D1 synthetic peptide (coupled to carrier protein) corresponds to amino acids in the semaphorin domain from human Plexin D1. This sequence is well conserved in rat and mouse Plexin D1, and has low homology to other Plexin family members.
• Preparation and Storage: Store at -20 degree C. Stable for 1 year.

Western Blot (WB)

(Western blot analysis of Plexin D1 expression in human endothelial cells (HUVEC) (lanes 1-6). The blots were probed with rabbit polyclonals anti-Plexin D1 (Sema domain) (lanes 1 & 2), anti-Plexin D1 (Cytoplasmic domain) (lanes 3 & 4), and anti-Plexin D1 (a.a. 1635-1647) (lanes 5 & 6). Each antibody was used in the presence of their respective blocking peptide (lanes 2, 4 & 6). )

Related Product Information for anti-PLXND1 antibody

Plexins are a family of large integral membrane proteins that complex with neuropilins to form semaphorin co-receptors. The extracellular region of plexins contains a semaphorin domain, multiple glycine-rich motifs, and MET-related sequences. The cytoplasmic region contains a Sex/Plexin domain and putative tyrosine phosphorylation sites that mediate signal transduction after activation. Plexin D1 is a type I transmembrane protein that may be expressed as multiple isoforms in many cell types, including neurons and endothelial cells. Semaphorin 3E (Sema-3E) and semaphorin 4A can bind Plexin D1, and ligand binding leads to phosphorylation of Tyr-1642 and activation of Plexin D1. Sema-3E and its receptor are important for angiogenesis that occurs during blood vessel development and repair. In cancers, Sema-3E and Plexin D1 are expressed in tumor tissues and cancer cells, and the interaction of these molecules may promote cancer cell migration and metastic spreading. Thus, Plexin D1, and its ligand Sema-3E, may be important regulators of angiogenesis and metastasis.

References

van der Zwaag, B. et al. (2002) Dev Dyn. 2002 Nov;225(3):336-43.Deutsch, U. (2004) Dev Cell. 7(1):1.Casazza, A. et al. (2010) J Clin Invest. 120(8):2684.

NCBI and Uniprot Product Information

• NCBI GI #: 38383188
• NCBI GeneID: 23129
• UniProt Accession #: Q9Y4D7
• Molecular Weight: 250 kDa
• NCBI Official Full Name: PLXND1 protein
• NCBI Official Synonym Full Names: plexin D1
• NCBI Official Symbol: PLXND1
• NCBI Official Synonym Symbols: PLEXD1
• NCBI Protein Information: plexin-D1
• UniProt Protein Name: Plexin-D1
• Protein Family: Plexin
• UniProt Gene Name: PLXND1
• UniProt Synonym Gene Names: KIAA0620

Uniprot Description

Cell surface receptor for SEMA4A and for class 3 semaphorins, such as SEMA3A, SEMA3C and SEMA3E. Plays an important role in cell-cell signaling, and in regulating the migration of a wide spectrum of cell types. Regulates the migration of thymocytes in the medulla. Regulates endothelial cell migration. Plays an important role in ensuring the specificity of synapse formation. Required for normal development of the heart and vasculature (). Mediates anti-angiogenic signaling in response to SEMA3E.