Western Blot (WB)
(Western blot analysis of extracts from VERO cells, using Plexin D1 Antibody. The lane on the left was treated with blocking peptide.)
Rabbit Plexin D1 Polyclonal Antibody | anti-PLXND1 antibody
Plexin D1 Antibody
Gene Names
PLXND1; PLEXD1
Reactivity
Human, MonkeyPredicted Reactivity: Horse(100%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
Plexin D1; Polyclonal Antibody; Plexin D1 Antibody; KIAA0620; MGC75353; PLEX D1; PLEXD 1; PLEXD1; Plexin-D1; PlexinD1; PLXD1_HUMAN; PLXN D1; PLXND 1; PLXND1; PLXND1 protein; anti-PLXND1 antibody
Host
Rabbit
Reactivity
Human, Monkey
Predicted Reactivity: Horse(100%)
Predicted Reactivity: Horse(100%)
Clonality
Polyclonal
Isotype
IgG
Specificity
Plexin D1 Antibody detects endogenous levels of total Plexin D1.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
Concentration
1mg/ml (varies by lot)
Sequence Length
52
Applicable Applications for anti-PLXND1 antibody
ELISA (EIA)
Application Notes
WB: 1:500-1:2000
ELISA(peptide): 1:20,000-1:40,000
ELISA(peptide): 1:20,000-1:40,000
Immunogen
A synthesized peptide derived from human Plexin D1, corresponding to a region within the internal amino acids.
Fragment
Fab fragment
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from VERO cells, using Plexin D1 Antibody. The lane on the left was treated with blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from VERO cells, using Plexin D1 Antibody. The lane on the left was treated with blocking peptide.)
Related Product Information for anti-PLXND1 antibody
Function: Cell surface receptor for SEMA4A and for class 3 semaphorins, such as SEMA3A, SEMA3C and SEMA3E. Plays an important role in cell-cell signaling, and in regulating the migration of a wide spectrum of cell types. Regulates the migration of thymocytes in the medulla. Regulates endothelial cell migration. Plays an important role in ensuring the specificity of synapse formation. Required for normal development of the heart and vasculature (By similarity). Mediates anti-angiogenic signaling in response to SEMA3E.
Subcellular Location: Cell membrane>Single-pass membrane protein. Cell projection>Lamellipodium membrane.
Tissue Specificity: Detected at low levels in heart, placenta, lung, skeletal muscle, kidney, thymus and liver. Detected at very low levels in brain, colon, spleen, small intestine and peripheral blood leukocytes.
Subunit Structure: Interacts with NRP1 and SEMA4A (By similarity). Interacts with SH3BP1; they dissociate upon SEMA3E binding to PLXND1 allowing SH3BP1 to transduce downstream signal through RAC1 inactivation (PubMed:24841563).
Similarity: Belongs to the plexin family.
Subcellular Location: Cell membrane>Single-pass membrane protein. Cell projection>Lamellipodium membrane.
Tissue Specificity: Detected at low levels in heart, placenta, lung, skeletal muscle, kidney, thymus and liver. Detected at very low levels in brain, colon, spleen, small intestine and peripheral blood leukocytes.
Subunit Structure: Interacts with NRP1 and SEMA4A (By similarity). Interacts with SH3BP1; they dissociate upon SEMA3E binding to PLXND1 allowing SH3BP1 to transduce downstream signal through RAC1 inactivation (PubMed:24841563).
Similarity: Belongs to the plexin family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
Observed Molecular Weight: (Observed)212kD.
Predicted Molecular Weight: (Calculated)212kDa.
Predicted Molecular Weight: (Calculated)212kDa.
NCBI Official Full Name
PLXND1 protein
NCBI Official Synonym Full Names
plexin D1
NCBI Official Symbol
PLXND1
NCBI Official Synonym Symbols
PLEXD1
NCBI Protein Information
plexin-D1
UniProt Protein Name
Plexin-D1
Protein Family
UniProt Gene Name
PLXND1
UniProt Synonym Gene Names
KIAA0620
Uniprot Description
Cell surface receptor for SEMA4A and for class 3 semaphorins, such as SEMA3A, SEMA3C and SEMA3E. Plays an important role in cell-cell signaling, and in regulating the migration of a wide spectrum of cell types. Regulates the migration of thymocytes in the medulla. Regulates endothelial cell migration. Plays an important role in ensuring the specificity of synapse formation. Required for normal development of the heart and vasculature (). Mediates anti-angiogenic signaling in response to SEMA3E.
Research Articles on PLXND1
Similar Products
Product Notes
The PLXND1 plxnd1 (Catalog #AAA9612261) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Plexin D1 Antibody reacts with Human, Monkey Predicted Reactivity: Horse(100%) and may cross-react with other species as described in the data sheet. AAA Biotech's Plexin D1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). WB: 1:500-1:2000 ELISA(peptide): 1:20,000-1:40,000. Researchers should empirically determine the suitability of the PLXND1 plxnd1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Plexin D1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
