Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Plexin-D1 (PLXND1) Standard Curve.)
Human Plexin-D1 (PLXND1) ELISA Kit | PLXND1 elisa kit
Human Plexin-D1 (PLXND1) ELISA Kit
Gene Names
PLXND1; PLEXD1
Reactivity
Human
Synonyms
Plexin-D1 (PLXND1); Human Plexin-D1 (PLXND1) ELISA Kit; PLXND1; KIAA0620; MGC75353; PLEXD1; PLXND1 elisa kit
Reactivity
Human
Specificity
Human Plexin-D1 (PLXND1) ELISA Kit has high sensitivity and excellent specificity for detection of Human PLXND1. No significant cross-reactivity or interference between Human PLXND1 and analogues was observed.
Sequence Length
52
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Plexin-D1 (PLXND1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Plexin-D1 (PLXND1) Standard Curve.)
Related Product Information for PLXND1 elisa kit
Description: This Human Plexin-D1 (PLXND1) ELISA Kit employs a two-site sandwich ELISA to quantitate PLXND1 in samples. An antibody specific for PLXND1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PLXND1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLXND1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLXND1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Tamagnone et al. (1999) identified the cDNA sequence of a novel member of the plexin gene family and named the gene plexin D1. Plxnd1 was expressed by embryonic vascular endothelial cells. Expression was robust in endocardium, intersomitic vessels, pulmonary vasculature, aorta, and pharyngeal arch arteries. In adult mice, Plxnd1 was expressed in heart, brain, lung, kidney, and testis, consistent with broad expression in endothelial cells. Human vascular endothelial cells also expressed PLXND1. Gitler et al. (2004) demonstrated that mouse Plxnd1 heterodimerized with Npn1 to form a functional Sema3C receptor. Binding of Sema3A and Sema3C to COS cells expressing Npn1 was enhanced by cotransfection of Plxnd1. Plxnd1 also enhanced binding of Sema3C to Npn2.
Background: Tamagnone et al. (1999) identified the cDNA sequence of a novel member of the plexin gene family and named the gene plexin D1. Plxnd1 was expressed by embryonic vascular endothelial cells. Expression was robust in endocardium, intersomitic vessels, pulmonary vasculature, aorta, and pharyngeal arch arteries. In adult mice, Plxnd1 was expressed in heart, brain, lung, kidney, and testis, consistent with broad expression in endothelial cells. Human vascular endothelial cells also expressed PLXND1. Gitler et al. (2004) demonstrated that mouse Plxnd1 heterodimerized with Npn1 to form a functional Sema3C receptor. Binding of Sema3A and Sema3C to COS cells expressing Npn1 was enhanced by cotransfection of Plxnd1. Plxnd1 also enhanced binding of Sema3C to Npn2.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
PLXND1 protein
NCBI Official Synonym Full Names
plexin D1
NCBI Official Symbol
PLXND1
NCBI Official Synonym Symbols
PLEXD1
NCBI Protein Information
plexin-D1
UniProt Protein Name
Plexin-D1
Protein Family
UniProt Gene Name
PLXND1
UniProt Synonym Gene Names
KIAA0620
Uniprot Description
Cell surface receptor for SEMA4A and for class 3 semaphorins, such as SEMA3A, SEMA3C and SEMA3E. Plays an important role in cell-cell signaling, and in regulating the migration of a wide spectrum of cell types. Regulates the migration of thymocytes in the medulla. Regulates endothelial cell migration. Plays an important role in ensuring the specificity of synapse formation. Required for normal development of the heart and vasculature (). Mediates anti-angiogenic signaling in response to SEMA3E.
Research Articles on PLXND1
Similar Products
Product Notes
The Human PLXND1 plxnd1 (Catalog #AAA9716018) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716018 ELISA Kit recognizes Human PLXND1. It is sometimes possible for the material contained within the vial of "Plexin-D1 (PLXND1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
