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Immunohistochemistry (IHC) (Immunohistochemistry analysis using Rabbit Anti-PKC Gamma Polyclonal Antibody . Tissue: Cerebellum. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-PKC Gamma Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 20X. HRP-DAB Detection.)

Rabbit anti-Mouse; Rat PKC gamma Polyclonal Antibody | anti-PKC gamma antibody

PKC gamma Antibody: ATTO 488

Gene Names
PRKCG; PKCC; PKCG; SCA14; PKC-gamma
Reactivity
Mouse; Rat
Applications
Western Blot
Purity
Peptide Affinity Purified
Synonyms
PKC gamma; Polyclonal Antibody; PKC gamma Antibody: ATTO 488; Rabbit Anti-Human PRKCG Polyclonal; PKCG Antibody; PRKCG Antibody; PKC-gamma Antibody; Protein kinase C gamma type Antibody; EC:2.7.11.13 Antibody; anti-PKC gamma antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Mouse; Rat
Clonality
Polyclonal
Specificity
Detects 62 kDa, 40 kDa. 62 kDa band is isoform 2, band at 40 kDa is a degradation product.
Purity/Purification
Peptide Affinity Purified
Concentration
1 mg/ml (varies by lot)
Sequence Length
710
Applicable Applications for anti-PKC gamma antibody
Western Blot (WB)
Application Notes
WB: 1:1000
Target
PRKCG
Immunogen
Synthetic peptide from the mid-protein of Human PRKCG (aa. 150-250)
Immunogen Species
Human
Cellular Localization
Cytoplasm; Cytoplasm; Perinuclear Region; Cell Membrane; Peripheral Membrane Protein; Cell Junction; Synapse; Synaptosome; Cell Projection; Dendrite
Conjugate
ATTO 488
Storage Buffer
PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide
Tissue Specificity
Expressed in Purkinje cells of the cerebellar cortex.
Preparation and Storage
Store at -20 degree C.

Immunohistochemistry (IHC)

(Immunohistochemistry analysis using Rabbit Anti-PKC Gamma Polyclonal Antibody . Tissue: Cerebellum. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-PKC Gamma Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 20X. HRP-DAB Detection.)

Immunohistochemistry (IHC) (Immunohistochemistry analysis using Rabbit Anti-PKC Gamma Polyclonal Antibody . Tissue: Cerebellum. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-PKC Gamma Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 20X. HRP-DAB Detection.)

Western Blot (WB)

(Western blot analysis of Mouse Brain showing detection of 78 kDa PRKCG protein using Rabbit Anti-PRKCG Polyclonal Antibody . Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain . Load: 15 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-PRKCG Polyclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: 78 kDa. Other Band(s): 62 kDa, 40 kDa.)

Western Blot (WB) (Western blot analysis of Mouse Brain showing detection of 78 kDa PRKCG protein using Rabbit Anti-PRKCG Polyclonal Antibody . Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain . Load: 15 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-PRKCG Polyclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: 78 kDa. Other Band(s): 62 kDa, 40 kDa.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
62,030 Da
NCBI Official Full Name
protein kinase C gamma type isoform 1
NCBI Official Synonym Full Names
protein kinase C gamma
NCBI Official Symbol
PRKCG
NCBI Official Synonym Symbols
PKCC; PKCG; SCA14; PKC-gamma
NCBI Protein Information
protein kinase C gamma type
UniProt Protein Name
Protein kinase C gamma type
UniProt Gene Name
PRKCG
UniProt Synonym Gene Names
PKCG; PKC-gamma

NCBI Description

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. The protein encoded by this gene is one of the PKC family members. This protein kinase is expressed solely in the brain and spinal cord and its localization is restricted to neurons. It has been demonstrated that several neuronal functions, including long term potentiation (LTP) and long term depression (LTD), specifically require this kinase. Knockout studies in mice also suggest that this kinase may be involved in neuropathic pain development. Defects in this protein have been associated with neurodegenerative disorder spinocerebellar ataxia-14 (SCA14). Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2015]

Uniprot Description

Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that plays diverse roles in neuronal cells and eye tissues, such as regulation of the neuronal receptors GRIA4/GLUR4 and GRIN1/NMDAR1, modulation of receptors and neuronal functions related to sensitivity to opiates, pain and alcohol, mediation of synaptic function and cell survival after ischemia, and inhibition of gap junction activity after oxidative stress. Binds and phosphorylates GRIA4/GLUR4 glutamate receptor and regulates its function by increasing plasma membrane-associated GRIA4 expression. In primary cerebellar neurons treated with the agonist 3,5-dihyidroxyphenylglycine, functions downstream of the metabotropic glutamate receptor GRM5/MGLUR5 and phosphorylates GRIN1/NMDAR1 receptor which plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. May be involved in the regulation of hippocampal long-term potentiation (LTP), but may be not necessary for the process of synaptic plasticity. May be involved in desensitization of mu-type opioid receptor-mediated G-protein activation in the spinal cord, and may be critical for the development and/or maintenance of morphine-induced reinforcing effects in the limbic forebrain. May modulate the functionality of mu-type-opioid receptors by participating in a signaling pathway which leads to the phosphorylation and degradation of opioid receptors. May also contributes to chronic morphine-induced changes in nociceptive processing. Plays a role in neuropathic pain mechanisms and contributes to the maintenance of the allodynia pain produced by peripheral inflammation. Plays an important role in initial sensitivity and tolerance to ethanol, by mediating the behavioral effects of ethanol as well as the effects of this drug on the GABA(A) receptors. During and after cerebral ischemia modulate neurotransmission and cell survival in synaptic membranes, and is involved in insulin-induced inhibition of necrosis, an important mechanism for minimizing ischemic injury. Required for the elimination of multiple climbing fibers during innervation of Purkinje cells in developing cerebellum. Is activated in lens epithelial cells upon hydrogen peroxide treatment, and phosphorylates connexin-43 (GJA1/CX43), resulting in disassembly of GJA1 gap junction plaques and inhibition of gap junction activity which could provide a protective effect against oxidative stress (). Phosphorylates p53/TP53 and promotes p53/TP53-dependent apoptosis in response to DNA damage. Involved in the phase resetting of the cerebral cortex circadian clock during temporally restricted feeding. Stabilizes the core clock component ARNTL/BMAL1 by interfering with its ubiquitination, thus suppressing its degradation, resulting in phase resetting of the cerebral cortex clock ().

Research Articles on PKC gamma

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Product Notes

The PKC gamma prkcg (Catalog #AAA8001509) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PKC gamma Antibody: ATTO 488 reacts with Mouse; Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PKC gamma can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:1000. Researchers should empirically determine the suitability of the PKC gamma prkcg for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PKC gamma, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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