Human Protein kinase C gamma type ELISA Kit | PRKCG elisa kit

Human Protein kinase C gamma type ELISA Kit

Cat. Num. AAA761793

• Gene Names: PRKCG; PKCC; PKCG; SCA14; PKC-gamma
• Reactivity: Human
• Synonyms: Protein kinase C gamma type; Human Protein kinase C gamma type ELISA Kit; PRKCG/PKCC/PKCG/SCA14/PKC-gamma/ MGC57564/PKCCSCA14/PKCGprotein kinase C gamma type/protein kinase C; gamma; PRKCG elisa kit

• Reactivity: Human
• Sequence Length: 710

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for PRKCG elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human PRKCG antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human PRKCG antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human PRKCG amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human PRKCG can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 938403405
• NCBI GeneID: 5582
• NCBI Accession #: NP_001303258.1
• NCBI GenBank Nucleotide #: NM_001316329.1
• Molecular Weight: 62,030 Da
• NCBI Official Full Name: protein kinase C gamma type isoform 1
• NCBI Official Synonym Full Names: protein kinase C gamma
• NCBI Official Symbol: PRKCG
• NCBI Official Synonym Symbols: PKCC; PKCG; SCA14; PKC-gamma
• NCBI Protein Information: protein kinase C gamma type
• UniProt Protein Name: Protein kinase C gamma type
• Protein Family: Protein kinase
• UniProt Gene Name: PRKCG
• UniProt Synonym Gene Names: PKCG; PKC-gamma
• UniProt Entry Name: KPCG_HUMAN

NCBI Description

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. The protein encoded by this gene is one of the PKC family members. This protein kinase is expressed solely in the brain and spinal cord and its localization is restricted to neurons. It has been demonstrated that several neuronal functions, including long term potentiation (LTP) and long term depression (LTD), specifically require this kinase. Knockout studies in mice also suggest that this kinase may be involved in neuropathic pain development. Defects in this protein have been associated with neurodegenerative disorder spinocerebellar ataxia-14 (SCA14). Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2015]

Uniprot Description

PKCG: a calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase. Expressed in the brain and spinal cord where its localization is restricted to neurons. Several neuronal functions, including long term potentiation and depression (LTP&LTD) specifically require this kinase. Knockout studies in mice also suggest that this kinase may be involved in neuropathic pain development. Defects have been associated with neurodegenerative disorder spinocerebellar ataxia-14. Plays diverse roles in neuronal cells and eye tissues, such as regulation of the neuronal receptors GLUR4 and NMDAR1, modulation of receptors and neuronal functions related to sensitivity to opiates, pain and alcohol, mediation of synaptic function and cell survival after ischemia, and inhibition of gap junction activity after oxidative stress. Binds and phosphorylates GLUR4 glutamate receptor and regulates its function by increasing plasma membrane-associated GRIA4 expression. In primary cerebellar neurons treated with the agonist 3,5-dihyidroxyphenylglycine, functions downstream of the metabotropic glutamate receptor MGLUR5 and phosphorylates NMDAR1 receptor which plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. May be involved in the regulation of hippocampal long-term potentiation (LTP), but may be not necessary for the process of synaptic plasticity. May modulate the functionality of mu-type-opioid receptors by participating in a signaling pathway which leads to the phosphorylation and degradation of opioid receptors. May also contributes to chronic morphine-induced changes in nociceptive processing. Plays a role in neuropathic pain mechanisms and contributes to the maintenance of the allodynia pain produced by peripheral inflammation. Plays an important role in initial sensitivity and tolerance to ethanol, by mediating the behavioral effects of ethanol as well as the effects of this drug on the GABA(A) receptors. During and after cerebral ischemia modulate neurotransmission and cell survival in synaptic membranes, and is involved in insulin-induced inhibition of necrosis, an important mechanism for minimizing ischemic injury. Required for the elimination of multiple climbing fibers during innervation of Purkinje cells in developing cerebellum. Is activated in lens epithelial cells upon hydrogen peroxide treatment, and phosphorylates connexin-43, resulting in disassembly of GJA1 gap junction plaques and inhibition of gap junction activity which could provide a protective effect against oxidative stress. Phosphorylates p53 and promotes p53-dependent apoptosis in response to DNA damage. Interacts with GRIA4. Interacts with CDCP1. Interacts with TP53INP1 and p53. Expressed in Purkinje cells of the cerebellar cortex.

Protein type: EC 2.7.11.13; Kinase, protein; Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor); AGC group; PKC family; Alpha subfamily

Chromosomal Location of Human Ortholog: 19q13.4

Cellular Component: cytosol; dendrite; intercellular junction; intracellular; nucleus; perinuclear region of cytoplasm; plasma membrane

Molecular Function: ATP binding; calcium-dependent protein kinase C activity; protein kinase activity; protein kinase C activity; protein serine/threonine/tyrosine kinase activity; zinc ion binding

Biological Process: blood coagulation; chemosensory behavior; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; innate immune response; learning and/or memory; negative regulation of neuron apoptosis; negative regulation of protein catabolic process; negative regulation of protein ubiquitination; nerve growth factor receptor signaling pathway; peptidyl-serine phosphorylation; phospholipase C activation; phosphorylation; platelet activation; positive regulation of mismatch repair; protein amino acid autophosphorylation; protein amino acid phosphorylation; regulation of circadian rhythm; regulation of phagocytosis; regulation of response to food; response to morphine; response to pain; rhythmic process; signal transduction; synaptic transmission

Disease: Spinocerebellar Ataxia 14