Rabbit anti-Mouse; Rat PKC gamma Polyclonal Antibody | anti-PKC gamma antibody

PKC gamma Antibody: Biotin

Cat. Num. AAA8001518

• Gene Names: PRKCG; PKCC; PKCG; SCA14; PKC-gamma
• Reactivity: Mouse; Rat
• Applications: Western Blot
• Purity: Peptide Affinity Purified
• Synonyms: PKC gamma; Polyclonal Antibody; PKC gamma Antibody: Biotin; Rabbit Anti-Human PRKCG Polyclonal; PKCG Antibody; PRKCG Antibody; PKC-gamma Antibody; Protein kinase C gamma type Antibody; EC:2.7.11.13 Antibody; anti-PKC gamma antibody

• Host: Rabbit
• Reactivity: Mouse; Rat
• Clonality: Polyclonal
• Specificity: Detects 62 kDa, 40 kDa. 62 kDa band is isoform 2, band at 40 kDa is a degradation product.
• Purity/Purification: Peptide Affinity Purified
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 710

• Applicable Applications for anti-PKC gamma antibody: Western Blot (WB)
• Application Notes: WB: 1:1000
• Target: PRKCG
• Immunogen: Synthetic peptide from the mid-protein of Human PRKCG (aa. 150-250)
• Immunogen Species: Human
• Cellular Localization: Cytoplasm; Cytoplasm; Perinuclear Region; Cell Membrane; Peripheral Membrane Protein; Cell Junction; Synapse; Synaptosome; Cell Projection; Dendrite
• Conjugate: Biotin
• Storage Buffer: PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide
• Tissue Specificity: Expressed in Purkinje cells of the cerebellar cortex.
• Preparation and Storage: Store at -20 degree C.

Immunohistochemistry (IHC)

(Immunohistochemistry analysis using Rabbit Anti-PKC Gamma Polyclonal Antibody . Tissue: Cerebellum. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-PKC Gamma Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 20X. HRP-DAB Detection.)

Western Blot (WB)

(Western blot analysis of Mouse Brain showing detection of 78 kDa PRKCG protein using Rabbit Anti-PRKCG Polyclonal Antibody . Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain . Load: 15 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-PRKCG Polyclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: 78 kDa. Other Band(s): 62 kDa, 40 kDa.)

NCBI and Uniprot Product Information

• NCBI GI #: 938403405
• NCBI GeneID: 5582
• NCBI Accession #: NP_001303258.1
• NCBI GenBank Nucleotide #: NM_001316329.1
• UniProt Accession #: P05129; B7Z8Q0
• Molecular Weight: 62,030 Da
• NCBI Official Full Name: protein kinase C gamma type isoform 1
• NCBI Official Synonym Full Names: protein kinase C gamma
• NCBI Official Symbol: PRKCG
• NCBI Official Synonym Symbols: PKCC; PKCG; SCA14; PKC-gamma
• NCBI Protein Information: protein kinase C gamma type
• UniProt Protein Name: Protein kinase C gamma type
• UniProt Gene Name: PRKCG
• UniProt Synonym Gene Names: PKCG; PKC-gamma

NCBI Description

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. The protein encoded by this gene is one of the PKC family members. This protein kinase is expressed solely in the brain and spinal cord and its localization is restricted to neurons. It has been demonstrated that several neuronal functions, including long term potentiation (LTP) and long term depression (LTD), specifically require this kinase. Knockout studies in mice also suggest that this kinase may be involved in neuropathic pain development. Defects in this protein have been associated with neurodegenerative disorder spinocerebellar ataxia-14 (SCA14). Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2015]

Uniprot Description

Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that plays diverse roles in neuronal cells and eye tissues, such as regulation of the neuronal receptors GRIA4/GLUR4 and GRIN1/NMDAR1, modulation of receptors and neuronal functions related to sensitivity to opiates, pain and alcohol, mediation of synaptic function and cell survival after ischemia, and inhibition of gap junction activity after oxidative stress. Binds and phosphorylates GRIA4/GLUR4 glutamate receptor and regulates its function by increasing plasma membrane-associated GRIA4 expression. In primary cerebellar neurons treated with the agonist 3,5-dihyidroxyphenylglycine, functions downstream of the metabotropic glutamate receptor GRM5/MGLUR5 and phosphorylates GRIN1/NMDAR1 receptor which plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. May be involved in the regulation of hippocampal long-term potentiation (LTP), but may be not necessary for the process of synaptic plasticity. May be involved in desensitization of mu-type opioid receptor-mediated G-protein activation in the spinal cord, and may be critical for the development and/or maintenance of morphine-induced reinforcing effects in the limbic forebrain. May modulate the functionality of mu-type-opioid receptors by participating in a signaling pathway which leads to the phosphorylation and degradation of opioid receptors. May also contributes to chronic morphine-induced changes in nociceptive processing. Plays a role in neuropathic pain mechanisms and contributes to the maintenance of the allodynia pain produced by peripheral inflammation. Plays an important role in initial sensitivity and tolerance to ethanol, by mediating the behavioral effects of ethanol as well as the effects of this drug on the GABA(A) receptors. During and after cerebral ischemia modulate neurotransmission and cell survival in synaptic membranes, and is involved in insulin-induced inhibition of necrosis, an important mechanism for minimizing ischemic injury. Required for the elimination of multiple climbing fibers during innervation of Purkinje cells in developing cerebellum. Is activated in lens epithelial cells upon hydrogen peroxide treatment, and phosphorylates connexin-43 (GJA1/CX43), resulting in disassembly of GJA1 gap junction plaques and inhibition of gap junction activity which could provide a protective effect against oxidative stress (). Phosphorylates p53/TP53 and promotes p53/TP53-dependent apoptosis in response to DNA damage. Involved in the phase resetting of the cerebral cortex circadian clock during temporally restricted feeding. Stabilizes the core clock component ARNTL/BMAL1 by interfering with its ubiquitination, thus suppressing its degradation, resulting in phase resetting of the cerebral cortex clock ().