Western Blot (WB)
(Western blot analysis of lysates from 293,K562,PC-3 cell line (from left to right),using PIP5KL1 Antibody (N-term). MBS9201010 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane.)
Rabbit anti-Human PIP5KL1 Polyclonal Antibody | anti-PIP5KL1 antibody
PIP5KL1 Antibody (N-term)
Western Blot (WB)
(Western blot analysis of lysates from 293,K562,PC-3 cell line (from left to right),using PIP5KL1 Antibody (N-term). MBS9201010 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane.)
Western Blot (WB)
(Western blot analysis of lysates from 293,K562,PC-3 cell line (from left to right),using PIP5KL1 Antibody (N-term). MBS9201010 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane.)
Immunohistochemistry (IHC)
(PIP5KL1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PIP5KL1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(PIP5KL1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PIP5KL1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of PIP5KL1 Antibody (N-term) with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of PIP5KL1 Antibody (N-term) with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
lacks intrinsic lipid kinase activity but associates with type I
PIPKs (see PIP5K1A; MIM 603275) and may play a role in localization
of PIPK activity (Chang et al., 2004 [PubMed 14701839]).[supplied
by OMIM].
Lamesch, P., et al. Genomics 89(3):307-315(2007)
Chang, J.D., et al. J. Biol. Chem. 279(12):11672-11679(2004)
NCBI and Uniprot Product Information
NCBI Description
PIP5KL1 is a phosphoinositide kinase-like protein that lacks intrinsic lipid kinase activity but associates with type I PIPKs (see PIP5K1A; MIM 603275) and may play a role in localization of PIPK activity (Chang et al., 2004 [PubMed 14701839]).[supplied by OMIM, Jun 2009]
Uniprot Description
PIP5KL1: May act as a scaffold to localize and regulate type I PI(4)P 5-kinases to specific compartments within the cell, where they generate PI(4,5)P2 for actin nucleation, signaling and scaffold protein recruitment and conversion to PI(3,4,5)P3. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Kinase, lipid; EC 2.7.1.68; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 9q34.11
Cellular Component: cell projection; membrane; cytoplasm
Molecular Function: 1-phosphatidylinositol-4-phosphate 5-kinase activity; ATP binding
Biological Process: phosphoinositide phosphorylation
Research Articles on PIP5KL1
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Product Notes
The PIP5KL1 pip5kl1 (Catalog #AAA9201010) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 57-86. The PIP5KL1 Antibody (N-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PIP5KL1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF), Western Blot (WB). WB~~1:1000. Researchers should empirically determine the suitability of the PIP5KL1 pip5kl1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PIP5KL1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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