Rabbit anti-Human PIP5KL1 Polyclonal Antibody | anti-PIP5KL1 antibody

PIP5KL1, NT (PIP5KL1, Phosphatidylinositol 4-phosphate 5-kinase-like protein 1)

Cat. Num. AAA647909

• Gene Names: PIP5KL1; PIPKH; bA203J24.5; RP11-203J24.5
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: PIP5KL1; Polyclonal Antibody; NT (PIP5KL1; Phosphatidylinositol 4-phosphate 5-kinase-like protein 1); Anti -PIP5KL1; anti-PIP5KL1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-PIP5KL1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500; Immunohistochemistry: 1:50-100; Immunofluorescence: 1:10-50
• Immunogen: PIP5KL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 64-94 amino acids from the N-terminal region of human PIP5KL1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(PIP5KL1 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the PIP5KL1 antibody detected the PIP5KL1 protein (arrow).)

Immunohistochemistry (IHC)

(PIP5KL1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PIP5KL1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of PIP5KL1 Antibody (N-term) with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Related Product Information for anti-PIP5KL1 antibody

PIP5KL1 is a phosphoinositide kinase-like protein that lacks intrinsic lipid kinase activity but associates with type I PIPKs (see PIP5K1A; MIM 603275) and may play a role in localization of PIPK activity (Chang et al., 2004 [PubMed 14701839]).[supplied by OMIM].

Product Categories/Family for anti-PIP5KL1 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 206725454
• NCBI GeneID: 138429
• NCBI Accession #: NP_001128691.1
• NCBI GenBank Nucleotide #: NM_001135219.1
• UniProt Accession #: Q5T9C9; Q8IVS3
• Molecular Weight: 44,572 Da
• NCBI Official Full Name: phosphatidylinositol 4-phosphate 5-kinase-like protein 1 isoform 1
• NCBI Official Synonym Full Names: phosphatidylinositol-4-phosphate 5-kinase-like 1
• NCBI Official Symbol: PIP5KL1
• NCBI Official Synonym Symbols: PIPKH; bA203J24.5; RP11-203J24.5
• NCBI Protein Information: phosphatidylinositol 4-phosphate 5-kinase-like protein 1; PI(4)P 5-kinase-like protein 1; ptdIns(4)P-5-kinase-like protein 1; phosphatidylinositol phosphate kinase homolog; phosphatidylinositol-4-phosphate 5-kinase-like protein 1
• UniProt Protein Name: Phosphatidylinositol 4-phosphate 5-kinase-like protein 1
• Protein Family: Phosphatidylinositol 4-phosphate 5-kinase-like protein
• UniProt Gene Name: PIP5KL1
• UniProt Synonym Gene Names: PI(4)P 5-kinase-like protein 1
• UniProt Entry Name: PI5L1_HUMAN

NCBI Description

PIP5KL1 is a phosphoinositide kinase-like protein that lacks intrinsic lipid kinase activity but associates with type I PIPKs (see PIP5K1A; MIM 603275) and may play a role in localization of PIPK activity (Chang et al., 2004 [PubMed 14701839]).[supplied by OMIM, Jun 2009]

Uniprot Description

Function: May act as a scaffold to localize and regulate type I PI4P 5-kinases to specific compartments within the cell, where they generate PI(4,5)P2 for actin nucleation, signaling and scaffold protein recruitment and conversion to PI(3,4,5)P3

By similarity.

Catalytic activity: ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate = ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate.

Subunit structure: Heterodimerizes with other type I phosphatidylinositol 4-phosphate 5-kinase

By similarity.

Subcellular location: Cytoplasm. Membrane. Note: Localized to large cytoplasmic vesicular structures

By similarity.

Sequence similarities: Contains 1 PIPK domain.

Caution: It is unsure if the enzyme has intrinsic kinase activity.