Rabbit PI4K2A Polyclonal Antibody | anti-PI4K2A antibody

PI4K2A Antibody

Cat. Num. AAA9629350

• Gene Names: PI4K2A; PI4KII; PIK42A
• Reactivity: Human, Mouse, Rat
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: PI4K2A; Polyclonal Antibody; PI4K2A Antibody; Phosphatidylinositol 4 kinase type 2 alpha; Phosphatidylinositol 4 kinase type II alpha; PI4KII; anti-PI4K2A antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: PI4K2A Antibody detects endogenous levels of total PI4K2A.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-PI4K2A antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from Human MYOCD.
• Conjugation: Unconjugated
• Similarity: Belongs to the PI3/PI4-kinase family. Type II PI4K subfamily.
• Post Translational Modifications: Palmitoylated by ZDHHC3 and ZDHHC7 in the CCPCC motif. Palmitoylation is cholesterol-dependent, and required for TGN localization.Ubiquitinated by ITCH; this does not lead to proteasomal degradation.
• Subunit Structure: Associates with the BLOC-1 and the AP-3 complexes; the BLOC-1 complex is required for optimal binding of PI4K2A to the AP-3 complex. Interacts with BLOC1S5 and DTNBP1. Interacts with FOS; this interaction may enhance phosphatidylinositol phosphorylation activity (By similarity). Interacts with ITCH.
• Tissue Specificity: Widely expressed. Highest expression is observed in kidney, brain, heart, skeletal muscle, and placenta and lowest expression is observed in colon, thymus, and small intestine.
• Function: Membrane-bound phosphatidylinositol-4 kinase (PI4-kinase) that catalyzes the phosphorylation of phosphatidylinositol (PI) to phosphatidylinositol 4-phosphate (PI4P), a lipid that plays important roles in endocytosis, Golgi function, protein sorting and membrane trafficking and is required for prolonged survival of neurons. Besides, phosphorylation of phosphatidylinositol (PI) to phosphatidylinositol 4-phosphate (PI4P) is the first committed step in the generation of phosphatidylinositol 4, 5-bisphosphate (PIP2), a precursor of the second messenger inositol 1, 4, 5-trisphosphate (InsP3).
• Note: Found in subdomains of the plasma membrane termed non-caveolar membrane rafts. Transported from neuronal cell body to neuron projections and neurite tips in a BLOC-1- and AP-3-complexes-dependent manner.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-PI4K2A antibody

Golgi apparatus; trans-Golgi network membrane; Lipid-anchor; Membrane raft; Cell projection; Dendrite; Cell junction; Synapse; Presynaptic cell membrane; Synaptosome; Mitochondrion; Endosome; Cytoplasmic vesicle; Membrane; Cell membrane; Perikaryon; Neuro

NCBI and Uniprot Product Information

• NCBI GI #: 734520304
• NCBI GeneID: 55361
• NCBI Accession #: NM_018425.3
• NCBI GenBank Nucleotide #: NM_018425
• UniProt Accession #: Q9BTU6; Q9NSG8; D3DR59
• Molecular Weight: ~79 kDa
• NCBI Official Full Name: Homo sapiens phosphatidylinositol 4-kinase type 2 alpha (PI4K2A), mRNA
• NCBI Official Synonym Full Names: phosphatidylinositol 4-kinase type 2 alpha
• NCBI Official Symbol: PI4K2A
• NCBI Official Synonym Symbols: PI4KII; PIK42A
• NCBI Protein Information: phosphatidylinositol 4-kinase type 2-alpha
• UniProt Protein Name: Phosphatidylinositol 4-kinase type 2-alpha
• Protein Family: Phosphatidylinositol 4-kinase
• UniProt Gene Name: PI4K2A

NCBI Description

Phosphatidylinositolpolyphosphates (PtdInsPs) are centrally involved in many biologic processes, ranging from cell growth and organization of the actin cytoskeleton to endo- and exocytosis. PI4KII phosphorylates PtdIns at the D-4 position, an essential step in the biosynthesis of PtdInsPs (Barylko et al., 2001 [PubMed 11244087]).[supplied by OMIM, Mar 2008]

Uniprot Description

PI4K2A: Together with PI4K2B and the type III PI4Ks (PIK4CA and PIK4CB) it contributes to the overall PI4-kinase activity of the cell. The phosphorylation of phosphatidylinositol (PI) to PI4P is the first committed step in the generation of phosphatidylinositol 4,5-bisphosphate (PIP2), a precursor of the second messenger inositol 1,4,5-trisphosphate (InsP3). Contributes to the production of InsP3 in stimulated cells. Belongs to the PI3/PI4-kinase family. Type II PI4K subfamily.

Protein type: EC 2.7.1.67; Kinase, lipid; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 10q24.2

Cellular Component: cell soma; cytoplasmic vesicle; cytosol; dendrite; endosome; integral to plasma membrane; intrinsic to membrane; lipid raft; lysosomal membrane; membrane; mitochondrion; neuron projection; plasma membrane; presynaptic membrane; trans-Golgi network

Molecular Function: 1-phosphatidylinositol 4-kinase activity; ATP binding; protein binding

Biological Process: endosome organization and biogenesis; Golgi organization and biogenesis; phosphatidylinositol biosynthetic process; phosphoinositide phosphorylation