Western Blot (WB)
(PI4K2A Antibody (C-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the PI4K2A antibody detected the PI4K2A protein (arrow).)
Rabbit PI4K2A Polyclonal Antibody | anti-PI4K2A antibody
PI4K2A Antibody (C-term)
Western Blot (WB)
(PI4K2A Antibody (C-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the PI4K2A antibody detected the PI4K2A protein (arrow).)
Western Blot (WB)
(PI4K2A Antibody (C-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the PI4K2A antibody detected the PI4K2A protein (arrow).)
Western Blot (WB)
(All lanes : Anti-PI4K2A Antibody (C-term) at1:2000 dilution Lane 1: HepG2 whole cell lysateLane 2: Hela whole cell lysate Lane 3: HL-60whole cell lysate Lysates/proteins at 20 ?g perlane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution.Predicted band size : 54 kDa Blocking/Dilutionbuffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes : Anti-PI4K2A Antibody (C-term) at1:2000 dilution Lane 1: HepG2 whole cell lysateLane 2: Hela whole cell lysate Lane 3: HL-60whole cell lysate Lysates/proteins at 20 ?g perlane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution.Predicted band size : 54 kDa Blocking/Dilutionbuffer: 5% NFDM/TBST.)
centrally involved in many biologic processes, ranging from cell
growth and organization of the actin cytoskeleton to endo- and
exocytosis. PI4KII phosphorylates PtdIns at the D-4 position, an
essential step in the biosynthesis of PtdInsPs (Barylko et al.,
2001 [PubMed 11244087]).
Qin, Y., et al. J. Biol. Chem. 284(34):22544-22548(2009)
Salazar, G., et al. J. Biol. Chem. 284(3):1790-1802(2009)
Craige, B., et al. Mol. Biol. Cell 19(4):1415-1426(2008)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
NCBI and Uniprot Product Information
NCBI Description
Phosphatidylinositolpolyphosphates (PtdInsPs) are centrally involved in many biologic processes, ranging from cell growth and organization of the actin cytoskeleton to endo- and exocytosis. PI4KII phosphorylates PtdIns at the D-4 position, an essential step in the biosynthesis of PtdInsPs (Barylko et al., 2001 [PubMed 11244087]).[supplied by OMIM, Mar 2008]
Uniprot Description
PI4K2A: Together with PI4K2B and the type III PI4Ks (PIK4CA and PIK4CB) it contributes to the overall PI4-kinase activity of the cell. The phosphorylation of phosphatidylinositol (PI) to PI4P is the first committed step in the generation of phosphatidylinositol 4,5-bisphosphate (PIP2), a precursor of the second messenger inositol 1,4,5-trisphosphate (InsP3). Contributes to the production of InsP3 in stimulated cells. Belongs to the PI3/PI4-kinase family. Type II PI4K subfamily.
Protein type: EC 2.7.1.67; Kinase, lipid; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 10q24
Cellular Component: Golgi apparatus; neuron projection; mitochondrion; synaptic vesicle membrane; lysosomal membrane; integral to plasma membrane; dendrite; perikaryon; cytosol; lipid raft; presynaptic membrane; cell soma; membrane; early endosome membrane; intrinsic to membrane; cytoplasmic vesicle; cell junction; endosome
Molecular Function: 1-phosphatidylinositol 4-kinase activity; protein binding; magnesium ion binding; ATP binding
Biological Process: phosphoinositide phosphorylation; phospholipid metabolic process; basophil degranulation; phosphatidylinositol biosynthetic process
Research Articles on PI4K2A
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Product Notes
The PI4K2A pi4k2a (Catalog #AAA9211629) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 387-415. The PI4K2A Antibody (C-term) reacts with Human (Predicted Reactivity: Zebrafish, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's PI4K2A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the PI4K2A pi4k2a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PI4K2A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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