Rabbit eEF2K Polyclonal Antibody | anti-eEF2K antibody

eEF2K (Ser-398), phospho-specific Antibody

Cat. Num. AAA474350

• Gene Names: EEF2K; eEF-2K; HSU93850
• Reactivity: Human, Rat, Mouse
• Applications: Western Blot, ELISA
• Synonyms: eEF2K; Polyclonal Antibody; eEF2K (Ser-398); phospho-specific Antibody; phospho-specific; eEF2K 398; anti-eEF2K antibody

• Host: Rabbit
• Reactivity: Human, Rat, Mouse
• Clonality: Polyclonal
• Specificity: The antibody was cross adsorbed to unphosphorylated eEF2K (Ser-398) peptide before affinity purification using phospho-eEF2K (Ser-398) peptide (without carrier). This antibody detects a 105kDa* protein corresponding to the molecular mass of eEF2K on SDS-PAGE immunoblots of Jurkat cells treated with Calyculin A, and this reactivity is removed after lambda phosphatase treatment.
• Form/Format: Rabbit polyclonal, affinity-purified antibody is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.

• Applicable Applications for anti-eEF2K antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA: 1:2000; WB: 1:1000
• Immunogen: Phospho-eEF2K (Ser-398) synthetic peptide (coupled to carrier protein) corresponds to amino acids surrounding Ser-398 in human eEF2K. This sequence is well conserved in rat and mouse eEF2K, and has low homology to other proteins.
• Preparation and Storage: Store at -20 degree C. Stable for 1 year.

Western Blot (WB)

(Western blot image of human Jurkat cells treated with calyculin A (100nM) for 30 min. (lanes 1-6), then some lanes of the blot were treated with lambda phosphatase (lanes 2, 4 & 6). The blot was probed with rabbit polyclonals anti-eEF2K (C-terminus) (lanes 1 & 2), anti-eEF2K (Ser-445) phospho-specific (lanes 3 & 4), and anti-eEF2K (Ser-398) phospho-specific (lanes 5 & 6).)

Related Product Information for anti-eEF2K antibody

Eukaryotic elongation factor 2 (eEF2) catalyzes the translocation of peptidyl-tRNA from the A site to the P site on the ribosome. eEF2 kinase (eEF2K) phosphorylates and inactivates eEF2, resulting in the inhibition of peptide-chain elongation. eEF2K is normally dependent on Ca2+ ions and calmodulin, and can be activated by PKA in response to elevated cAMP levels during cell stress- or starvation-related conditions. Regulation of eEF2K occurs through phosphorylation at multiple sites. Ser-78 phosphorylation is required for calmodulin binding and eEF2K activity, while phosphorylation of Ser-500 is required for Ca2+/calmodulin-independent kinase activity. Thr-348 and Ser-445 are two of the major autophosphorylation sites required for kinase activity. Inhibitory phosphorylation may also regulate eEF2K, since phosphorylation at Ser-359 by SAPK4/p38delta causes inactivation of eEF2K. In addition, Ser-398 phosphorylation is required for eEFK2 induced autophagy. Thus, multisite phospho-regulation of eEF2K may be important for proper control of eEF2K activity and protein translation.

References

Cheng, Y. et al. (2013) Autophagy. 9(2):208.
Tavares, C.D. et al. (2012) Biochemistry. 51(11):2232.
Smith, E.M. & Proud, C.G. (2008) EMBO J. 27(7):1005.
Ryazanov, A.G. et al. (1997) PNAS. USA. 94:4884.

NCBI and Uniprot Product Information

• NCBI GI #: 9558749
• NCBI GeneID: 29904
• NCBI Accession #: NP_037434.1
• NCBI GenBank Nucleotide #: NM_013302.3
• UniProt Accession #: O00418; Q8N588
• Molecular Weight: 82,144 Da
• NCBI Official Full Name: eukaryotic elongation factor 2 kinase
• NCBI Official Synonym Full Names: eukaryotic elongation factor-2 kinase
• NCBI Official Symbol: EEF2K
• NCBI Official Synonym Symbols: eEF-2K; HSU93850
• NCBI Protein Information: eukaryotic elongation factor 2 kinase; eEF-2 kinase; elongation factor-2 kinase; eukaroytic elongation factor 2 kinase; calmodulin-dependent protein kinase III; calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase; calcium/calmodulin-depende
• UniProt Protein Name: Eukaryotic elongation factor 2 kinase
• Protein Family: Eukaryotic elongation factor 2 kinase
• UniProt Gene Name: EEF2K
• UniProt Synonym Gene Names: eEF-2 kinase; eEF-2K
• UniProt Entry Name: EF2K_HUMAN

NCBI Description

This gene encodes a highly conserved protein kinase in the calmodulin-mediated signaling pathway that links activation of cell surface receptors to cell division. This kinase is involved in the regulation of protein synthesis. It phosphorylates eukaryotic elongation factor 2 (EEF2) and thus inhibits the EEF2 function. The activity of this kinase is increased in many cancers and may be a valid target for anti-cancer treatment. [provided by RefSeq, Jul 2008]

Uniprot Description

EEF2K: an atypical protein kinase of the Alpha family. Dependent on Ca2 ions and calmodulin. May play a key role linking cellular energy status and the inhibition of protein synthesis, a major consumer of metabolic energy. Phosphorylates and inactivates eEF2, resulting in the inhibition of peptide-chain elongation eEF2K is phosphorylated and activated by AMPK in response to elevated AMP levels, which increase during cellular stress and starvation. Regulated in response to a range of stimuli including TNF-alpha. Overexpressed in breast cancer cell lines and tumors. Activated in rat glioblastoma, and growth was inhibited by the drug Rottlerin. Activity induced in ischemic heart muscle

Protein type: Kinase, protein; Translation; EC 2.7.11.20; Protein kinase, atypical; Protein kinase, Ser/Thr (non-receptor); ATYPICAL group; Alpha family; eEF2K subfamily

Chromosomal Location of Human Ortholog: 16p12.2

Cellular Component: cytoplasm; cytosol

Molecular Function: calmodulin binding; elongation factor-2 kinase activity; translation factor activity, nucleic acid binding; calcium ion binding; ATP binding; protein kinase activity

Biological Process: translational elongation; protein amino acid autophosphorylation; regulation of protein amino acid autophosphorylation; insulin receptor signaling pathway