Western Blot (WB)
(Western blot of HeLa cells treated with pervanadate (1mM) for 30 min. Phosphotyrosine containing proteins were immunoprecipitated with rabbit polyclonal anti-Phosphotyrosine:Agarose (Lane 1) or with no antibody agarose beads (Lane 2), and blots were made that included the whole lysate (Lane 3). The blots were probed with mouse monoclonal anti-Phosphotyrosine (PM3751) to detect phosphotyrosine containing proteins.)
Mouse Phosphotyrosine Monoclonal Antibody
Phosphotyrosine M375 Antibody
Reactivity
Human, Rat, Mouse, Chicken
Applications
Western Blot, ELISA, Immunoprecipitation, Immunocytochemistry
Purity
Protein A chromatography
Synonyms
Phosphotyrosine; Monoclonal Antibody; Phosphotyrosine M375 Antibody; Anti-Phosphotyrosine; Phosphotyrosine mAb; anti-Phosphotyrosine antibody
Host
Mouse
Reactivity
Human, Rat, Mouse, Chicken
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
M375
Specificity
This antibody detects phosphotyrosine-containing proteins on SDS-PAGE immunoblots of EGF treated A431 cells, as well as pervanadate treated A431, HeLa, Jurkat, and endothelial cells.
Purity/Purification
Protein A chromatography
Form/Format
Mouse monoclonal antibody purified with protein A chromatography is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.
Applicable Applications for anti-Phosphotyrosine antibody
Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP), Immunocytochemistry (ICC)
Application Notes
ELISA: 1:2000
ICC: 1:100
IP: 1:100
WB: 1:1000
ICC: 1:100
IP: 1:100
WB: 1:1000
Immunogen
Clone M375 was generated from tyrosine phosphorylated proteins purified from pervanadate treated A431 cells.
Preparation and Storage
Store at -20 degree C. Stable for 1 year.
Western Blot (WB)
(Western blot of HeLa cells treated with pervanadate (1mM) for 30 min. Phosphotyrosine containing proteins were immunoprecipitated with rabbit polyclonal anti-Phosphotyrosine:Agarose (Lane 1) or with no antibody agarose beads (Lane 2), and blots were made that included the whole lysate (Lane 3). The blots were probed with mouse monoclonal anti-Phosphotyrosine (PM3751) to detect phosphotyrosine containing proteins.)
Western Blot (WB)
(Western blot of HeLa cells treated with pervanadate (1mM) for 30 min. Phosphotyrosine containing proteins were immunoprecipitated with rabbit polyclonal anti-Phosphotyrosine:Agarose (Lane 1) or with no antibody agarose beads (Lane 2), and blots were made that included the whole lysate (Lane 3). The blots were probed with mouse monoclonal anti-Phosphotyrosine (PM3751) to detect phosphotyrosine containing proteins.)
Immunocytochemistry (ICC)
(Immunocytochemical labeling of phosphotyrosine in control and pervanadate-treated A431 cells. The cells were labeled with rabbit polyclonal anti-Phosphotyrosine (PP2221) and mouse monoclonal anti-Phosphotyrosine (PM3751), then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.)
Immunocytochemistry (ICC)
(Immunocytochemical labeling of phosphotyrosine in control and pervanadate-treated A431 cells. The cells were labeled with rabbit polyclonal anti-Phosphotyrosine (PP2221) and mouse monoclonal anti-Phosphotyrosine (PM3751), then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.)
Related Product Information for anti-Phosphotyrosine antibody
Phosphorylation of specific tyrosine residues is an important post-translational modification for regulating the activity of most proteins. Stimulation of a variety of cell signaling pathways activates the receptor and non-receptor tyrosine kinases that mediate these protein modifications. Antibodies that can detect phosphotyrosine residues are excellent tools for characterizing changes in the post-translational state of a broad range of phosphotyrosine-containing proteins. Immunoprecipitation of proteins of interest, followed by detection of phosphotyrosine using anti-phosphotyrosine antibody is commonly used to correlate changes in tyrosine phosphorylation state with alterations in protein activity.
References
Wang, J.Y.J. (1988) Anal. Biochem 172:1.
Hunter T.(1987) Cell. 50(6):823.
Ross, A.H. et al. (1981) Nature 294:654.
Hunter T.(1987) Cell. 50(6):823.
Ross, A.H. et al. (1981) Nature 294:654.
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Product Notes
The Phosphotyrosine (Catalog #AAA474519) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Phosphotyrosine M375 Antibody reacts with Human, Rat, Mouse, Chicken and may cross-react with other species as described in the data sheet. AAA Biotech's Phosphotyrosine can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP), Immunocytochemistry (ICC). ELISA: 1:2000 ICC: 1:100 IP: 1:100 WB: 1:1000. Researchers should empirically determine the suitability of the Phosphotyrosine for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Phosphotyrosine, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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