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Western Blot (WB) (Western blot analysis of extracts of various cell lines, using TRIB1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.)

Rabbit anti-Human TRIB1 Polyclonal Antibody | anti-TRIB1 antibody

TRIB1 Polyclonal Antibody

Gene Names
TRIB1; C8FW; GIG2; TRB1; GIG-2; SKIP1; TRB-1
Reactivity
Human
Applications
Western Blot
Purity
Affinity purification
Synonyms
TRIB1; Polyclonal Antibody; TRIB1 Polyclonal Antibody; anti-TRIB1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Sequence Length
206
Applicable Applications for anti-TRIB1 antibody
Western Blot (WB)
Application Notes
WB: 1:1000 - 1:4000
Immunogen
Recombinant protein of human TRIB1
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using TRIB1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.)

Western Blot (WB) (Western blot analysis of extracts of various cell lines, using TRIB1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.)
Product Categories/Family for anti-TRIB1 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Calculated MW: 41kDa
Molecular Weight: 372
NCBI Official Full Name
tribbles homolog 1 isoform 2
NCBI Official Synonym Full Names
tribbles pseudokinase 1
NCBI Official Symbol
TRIB1
NCBI Official Synonym Symbols
C8FW; GIG2; TRB1; GIG-2; SKIP1; TRB-1
NCBI Protein Information
tribbles homolog 1
UniProt Protein Name
Tribbles homolog 1
Protein Family
UniProt Gene Name
TRIB1
UniProt Synonym Gene Names
TRB-1; GIG-2
UniProt Entry Name
TRIB1_HUMAN

Uniprot Description

TRB1: Interacts with MAPK kinases and regulates activation of MAP kinases. May not display kinase activity. Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. Tribbles subfamily.

Protein type: Protein kinase, CAMK; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); CAMK group; Trbl family

Chromosomal Location of Human Ortholog: 8q24.13

Cellular Component: cytoplasm; nucleus

Molecular Function: ATP binding; mitogen-activated protein kinase kinase binding; protein binding; protein kinase activity; protein kinase inhibitor activity; transcription factor binding; ubiquitin protein ligase binding; ubiquitin-protein ligase regulator activity

Biological Process: JNK cascade; negative regulation of lipopolysaccharide-mediated signaling pathway; negative regulation of neutrophil differentiation; negative regulation of protein kinase activity; negative regulation of smooth muscle cell migration; negative regulation of smooth muscle cell proliferation; negative regulation of transcription factor activity; positive regulation of eosinophil differentiation; positive regulation of macrophage differentiation; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; protein amino acid phosphorylation; regulation of MAP kinase activity; response to lipopolysaccharide

Research Articles on TRIB1

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Product Notes

The TRIB1 trib1 (Catalog #AAA9129759) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TRIB1 Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TRIB1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:1000 - 1:4000. Researchers should empirically determine the suitability of the TRIB1 trib1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TRIB1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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