Western Blot (WB)
(Western blot analysis of MBS625383 (Center) in MDA-MB231, Hela cell line lysates (35ug/lane). EGLN2 (arrow) was detected using the purified Pab.)
Rabbit anti-Human PHD1, IN Polyclonal Antibody | anti-PHD1 antibody
PHD1, IN (Egl Nine Homolog 2, Hypoxia-inducible Factor Prolyl Hydroxylase 1, HIF-prolyl Hydroxylase 1, HIF-PH1, HPH-3, Prolyl Hydroxylase Domain-containing Protein 1, EGLN2, Estrogen-induced Tag 6, EIT6)
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Affinity PurifiedPurified by Protein A affinity chromatography
Synonyms
PHD1; IN; Polyclonal Antibody; IN (Egl Nine Homolog 2; Hypoxia-inducible Factor Prolyl Hydroxylase 1; HIF-prolyl Hydroxylase 1; HIF-PH1; HPH-3; Prolyl Hydroxylase Domain-containing Protein 1; EGLN2; Estrogen-induced Tag 6; EIT6); Anti -PHD1; anti-PHD1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human PHD1.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography
Purified by Protein A affinity chromatography
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide.
Applicable Applications for anti-PHD1 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Suitable for use in Immunohistochemistry, ELISA and Western Blot.
Dilution: Immunohistochemistry: 1:50-1:100. Formalin-fixed and paraffin-embedded human testis tissue reacted with P3373-05E, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
ELISA: 1:1000
Western Blot: 1:100-1:500
Dilution: Immunohistochemistry: 1:50-1:100. Formalin-fixed and paraffin-embedded human testis tissue reacted with P3373-05E, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
ELISA: 1:1000
Western Blot: 1:100-1:500
Immunogen
Synthetic peptide selected from the Center region of human PHD1 conjugated to KLH.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western blot analysis of MBS625383 (Center) in MDA-MB231, Hela cell line lysates (35ug/lane). EGLN2 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of MBS625383 (Center) in MDA-MB231, Hela cell line lysates (35ug/lane). EGLN2 (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human testis tissue reacted with MBS625383 which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human testis tissue reacted with MBS625383 which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.)
Flow Cytometry (FC/FACS)
(Flow Cytometry analysis of MDA-MB231 cells (right histogram) using MBS625383 compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(Flow Cytometry analysis of MDA-MB231 cells (right histogram) using MBS625383 compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-PHD1 antibody
PHD1 catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF-1 alpha at 'Pro-402' and 'Pro-564', and HIF-2 alpha. It functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. It may play a role in cell growth regulation.
Product Categories/Family for anti-PHD1 antibody
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
40,646 Da
NCBI Official Full Name
PHD1
UniProt Protein Name
Putative transcription factor PHD1
Protein Family
UniProt Gene Name
PHD1
UniProt Entry Name
PHD1_YEAST
Uniprot Description
Function: Putative transcription factor that functions in pseudohyphal growth.
Subcellular location: Nucleus.
Miscellaneous: Present with 1420 molecules/cell in log phase SD medium. Ref.7
Sequence similarities: Belongs to the EFG1/PHD1/stuA family.Contains 1 HTH APSES-type DNA-binding domain.
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Product Notes
The PHD1, IN phd1 (Catalog #AAA625383) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PHD1, IN (Egl Nine Homolog 2, Hypoxia-inducible Factor Prolyl Hydroxylase 1, HIF-prolyl Hydroxylase 1, HIF-PH1, HPH-3, Prolyl Hydroxylase Domain-containing Protein 1, EGLN2, Estrogen-induced Tag 6, EIT6) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PHD1, IN can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in Immunohistochemistry, ELISA and Western Blot. Dilution: Immunohistochemistry: 1:50-1:100. Formalin-fixed and paraffin-embedded human testis tissue reacted with P3373-05E, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. ELISA: 1:1000 Western Blot: 1:100-1:500. Researchers should empirically determine the suitability of the PHD1, IN phd1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PHD1, IN, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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