Mouse anti-Human EGLN2 Monoclonal Antibody | anti-EGLN2 antibody

EGLN2 (Egl Nine Homolog 2, Hypoxia-inducible Factor Prolyl Hydroxylase 1, HIF-prolyl Hydroxylase 1, HIF-PH1, HPH-3, Prolyl Hydroxylase Domain-containing Protein 1, PHD1, Estrogen-induced Tag 6, EIT6, DKFZp434E026) (FITC)

Cat. Num. AAA6146978

• Gene Names: EGLN2; EIT6; PHD1; EIT-6; HPH-1; HPH-3; HIFPH1; HIF-PH1
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: EGLN2; Monoclonal Antibody; EGLN2 (Egl Nine Homolog 2; Hypoxia-inducible Factor Prolyl Hydroxylase 1; HIF-prolyl Hydroxylase 1; HIF-PH1; HPH-3; Prolyl Hydroxylase Domain-containing Protein 1; PHD1; Estrogen-induced Tag 6; EIT6; DKFZp434E026) (FITC); anti-EGLN2 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 2G3
• Specificity: Recognizes human EGLN2.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
• Sequence Length: 2165

• Applicable Applications for anti-EGLN2 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length recombinant corresponding to aa1-408 from human EGLN2 (AAH36051) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: MDSPCQPQPLSQALPQLPGSSSEPLEPEPGRARMGVESYLPCPLLPSYHCPGVPSEASAGSGTPRATATSTTASPLRDGFGGQDGGELRPLQSEGAAALVTKGCQRLAAQGARPEAPKRKWAEDGGDAPSPSKRPWARQENQEAEREGGMSCSCSSGSGEASAGLMEEALPSAPERLALDYIVPCMRYYGICVKDSFLGAALGGRVLAEVEALKRGGRLRDGQLVSQRAIPPRSIRGDQIAWVEGHEPGCRSIGALMAHVDAVIRHCAGRLGSYVINGRTKAMVACYPGNGLGYVRHVDNPHGDGRCITCIYYLNQNWDVKVHGGLLQIFPEGRPVVANIEPLFDRLLIFWSDRRNPHEVKPAYATRYAITVWYFDAKERAAAKDKYQLASGQKGVQVPVSQPPTPT
• Conjugate: FITC
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (70.88kD).)

Testing Data

(Detection limit for recombinant GST tagged EGLN2 is 0.3ng/ml as a capture antibody.)

Related Product Information for anti-EGLN2 antibody

Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF2A. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Under hypoxic conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes. EGLN2 is involved in regulating hypoxia tolerance and apoptosis in cardiac and skeletal muscle. Also regulates susceptibility to normoxic oxidative neuronal death.

Product Categories/Family for anti-EGLN2 antibody

Antibodies; Hypoxia

NCBI and Uniprot Product Information

• NCBI GI #: 23273571
• NCBI GeneID: 112398
• NCBI Official Full Name: Homo sapiens egl nine homolog 2 (C. elegans), mRNA
• NCBI Official Synonym Full Names: egl-9 family hypoxia inducible factor 2
• NCBI Official Symbol: EGLN2
• NCBI Official Synonym Symbols: EIT6; PHD1; EIT-6; HPH-1; HPH-3; HIFPH1; HIF-PH1
• NCBI Protein Information: egl nine homolog 2
• Protein Family: Egl nine

NCBI Description

The hypoxia inducible factor (HIF) is a transcriptional complex that is involved in oxygen homeostasis. At normal oxygen levels, the alpha subunit of HIF is targeted for degration by prolyl hydroxylation. This gene encodes an enzyme responsible for this post-translational modification. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the upstream RAB4B (RAB4B, member RAS oncogene family) gene. [provided by RefSeq, Feb 2011]