Western Blot (WB)
(Western Blot (WB) analysis of PHD1 Rabbit mAb at 1:1000 dilutionLane1:Hela whole cell lysateLane2:PC12 whole cell lysateLane3:NIH/3T3 whole cell lysate)
Rabbit PHD1 Monoclonal Antibody | anti-PHD1 antibody
PHD1 Rabbit Monoclonal Antibody
ICC: 1:50-1:200
IHC: 1:50-1:200
IF: 1:50-1:200
FC/FACS: 1:50-1:100
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot (WB) analysis of PHD1 Rabbit mAb at 1:1000 dilutionLane1:Hela whole cell lysateLane2:PC12 whole cell lysateLane3:NIH/3T3 whole cell lysate)
Western Blot (WB)
(Western Blot (WB) analysis of PHD1 Rabbit mAb at 1:1000 dilutionLane1:Hela whole cell lysateLane2:PC12 whole cell lysateLane3:NIH/3T3 whole cell lysate)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-PHD1 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-PHD1 antibody. Counter stained with hematoxylin.)
Immunocytochemistry (ICC)
(ICC staining PHD1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.)
Immunocytochemistry (ICC)
(ICC staining PHD1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-PHD1 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-PHD1 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-PHD1 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-PHD1 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded mouse prostate tissue using anti-PHD1 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded mouse prostate tissue using anti-PHD1 antibody. Counter stained with hematoxylin.)
Immunocytochemistry (ICC)
(ICC staining PHD1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.)
Immunocytochemistry (ICC)
(ICC staining PHD1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.)
Immunocytochemistry (ICC)
(ICC staining PHD1 in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.)
Immunocytochemistry (ICC)
(ICC staining PHD1 in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Hela cells with PHD1 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Hela cells with PHD1 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
NCBI and Uniprot Product Information
NCBI Description
The hypoxia inducible factor (HIF) is a transcriptional complex that is involved in oxygen homeostasis. At normal oxygen levels, the alpha subunit of HIF is targeted for degration by prolyl hydroxylation. This gene encodes an enzyme responsible for this post-translational modification. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the upstream RAB4B (RAB4B, member RAS oncogene family) gene. [provided by RefSeq, Feb 2011]
Uniprot Description
EGLN2: Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF2A. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Under hypoxic conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes. EGLN2 is involved in regulating hypoxia tolerance and apoptosis in cardiac and skeletal muscle. Also regulates susceptibility to normoxic oxidative neuronal death. 2 isoforms of the human protein are produced by alternative initiation.
Protein type: EC 1.14.11.29; Oxidoreductase
Chromosomal Location of Human Ortholog: 19q13.2
Cellular Component: nucleoplasm; cytosol; nucleus
Molecular Function: protein binding; oxygen sensor activity; L-ascorbic acid binding; ferrous iron binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors; peptidyl-proline 4-dioxygenase activity
Biological Process: estrogen receptor signaling pathway; cell redox homeostasis; regulation of neuron apoptosis; positive regulation of protein catabolic process; response to hypoxia; peptidyl-proline hydroxylation to 4-hydroxy-L-proline; regulation of cell growth
Research Articles on PHD1
Similar Products
Product Notes
The PHD1 egln2 (Catalog #AAA3014104) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PHD1 Rabbit Monoclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PHD1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB: 1:1000-1:5000 ICC: 1:50-1:200 IHC: 1:50-1:200 IF: 1:50-1:200 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the PHD1 egln2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PHD1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
