Western Blot (WB)
(PGPEP1 Antibody (N-term) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the PGPEP1 antibody detected the PGPEP1 protein (arrow).)
Rabbit anti-Mouse PGPEP1 Polyclonal Antibody | anti-PGPEP1 antibody
PGPEP1, NT (PGPEP1, PGPI, Pyroglutamyl-peptidase 1, 5-oxoprolyl-peptidase, Pyroglutamyl aminopeptidase I, Pyroglutamyl-peptidase I, Pyrrolidone-carboxylate peptidase) (AP)
Reactivity
Mouse
Applications
Western Blot, ELISA
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
PGPEP1; Polyclonal Antibody; NT (PGPEP1; PGPI; Pyroglutamyl-peptidase 1; 5-oxoprolyl-peptidase; Pyroglutamyl aminopeptidase I; Pyroglutamyl-peptidase I; Pyrrolidone-carboxylate peptidase) (AP); Pyrrolidone-carboxylate peptidase; anti-PGPEP1 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Applicable Applications for anti-PGPEP1 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:100-500
ELISA: 1:1,000
Applications are based on unconjugated antibody.
ELISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
PGPEP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 29-59 amino acids from the N-terminal region of human PGPEP1.
Conjugate
AP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(PGPEP1 Antibody (N-term) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the PGPEP1 antibody detected the PGPEP1 protein (arrow).)
Western Blot (WB)
(PGPEP1 Antibody (N-term) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the PGPEP1 antibody detected the PGPEP1 protein (arrow).)
Related Product Information for anti-PGPEP1 antibody
Pyroglutamyl peptidase I (EC 3.4.19.3) catalyzes the hydrolysis of N-terminal pyroglutamyl residues from oligopeptides and proteins.
Product Categories/Family for anti-PGPEP1 antibody
References
Rose, J. Phd, et al. Mol. Med. (2010). In press: Valdivia, A., et al. Regul. Pept. 122(2):79-84(2004). Minderman, H., et al. Clin. Cancer Res. 10(5):1826-1834(2004). Dando, P.M., et al. Protein Expr. Purif. 28(1):111-119(2003). Gil, J., et al. Neuropeptides 35 (5-6), 276-284 (2001).
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
pyroglutamyl-peptidase 1 isoform 1
UniProt Protein Name
Pyroglutamyl-peptidase 1
Protein Family
UniProt Gene Name
PGPEP1
UniProt Synonym Gene Names
PGPI; PAP-I; PGP-I
UniProt Entry Name
PGPI_HUMAN
Uniprot Description
PGPEP1: Removes 5-oxoproline from various penultimate amino acid residues except L-proline. Belongs to the peptidase C15 family.
Protein type: EC 3.4.19.3; Protease
Chromosomal Location of Human Ortholog: 19p13.11
Cellular Component: cytosol
Molecular Function: pyroglutamyl-peptidase activity; cysteine-type peptidase activity
Biological Process: proteolysis
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Product Notes
The PGPEP1 pgpep1 (Catalog #AAA6333112) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PGPEP1, NT (PGPEP1, PGPI, Pyroglutamyl-peptidase 1, 5-oxoprolyl-peptidase, Pyroglutamyl aminopeptidase I, Pyroglutamyl-peptidase I, Pyrrolidone-carboxylate peptidase) (AP) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PGPEP1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:100-500 ELISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the PGPEP1 pgpep1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PGPEP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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