Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using POLD1 antibody.)
Rabbit POLD1 Polyclonal Antibody | anti-POLD1 antibody
POLD1 (DNA Polymerase delta Catalytic Subunit 125kD, CDC2, MDPL, POLD, CRCS10) (APC)
WB: 1:500-1:2000
IHC: 1:50-1:200
Applications are based on unconjugated antibody.
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using POLD1 antibody.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using POLD1 antibody.)
Immunofluorescence (IF)
(Immunofluorescence analysis of U2OS cell using POLD1 antibody. Blue: DAPI for nuclear staining.)
Immunofluorescence (IF)
(Immunofluorescence analysis of U2OS cell using POLD1 antibody. Blue: DAPI for nuclear staining.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes the 125-kDa catalytic subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3' to 5' exonuclease activity and plays a critical role in DNA replication and repair. Alternatively spliced transcript variants have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 6. [provided by RefSeq, Mar 2012]
Uniprot Description
POLD1: Possesses two enzymatic activities: DNA synthesis (polymerase) and an exonucleolytic activity that degrades single stranded DNA in the 3'- to 5'-direction. Required with its accessory proteins (proliferating cell nuclear antigen (PCNA) and replication factor C (RFC) or activator 1) for leading strand synthesis. Also involved in completing Okazaki fragments initiated by the DNA polymerase alpha/primase complex. Belongs to the DNA polymerase type-B family.
Protein type: EC 2.7.7.7; Transferase; DNA repair, damage; Nucleotide Metabolism - pyrimidine; Hydrolase; Nucleotide Metabolism - purine; DNA replication
Chromosomal Location of Human Ortholog: 19q13.3
Cellular Component: nucleoplasm; delta DNA polymerase complex; nucleotide-excision repair complex; membrane; cytoplasm; nucleus
Molecular Function: protein binding; DNA binding; metal ion binding; 4 iron, 4 sulfur cluster binding; nucleotide binding; DNA-directed DNA polymerase activity; 3'-5'-exodeoxyribonuclease activity; chromatin binding
Biological Process: mismatch repair; DNA strand elongation during DNA replication; DNA repair; base-excision repair, gap-filling; telomere maintenance via semi-conservative replication; DNA synthesis during DNA repair; DNA replication proofreading; nucleotide-excision repair; base-excision repair; fatty acid homeostasis; transcription-coupled nucleotide-excision repair; telomere maintenance via recombination; mitotic cell cycle; nucleotide-excision repair, DNA gap filling; DNA replication; DNA catabolic process, exonucleolytic; telomere maintenance; response to UV
Disease: Colorectal Cancer, Susceptibility To, 10; Mandibular Hypoplasia, Deafness, Progeroid Features, And Lipodystrophy Syndrome
Research Articles on POLD1
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Product Notes
The POLD1 pold1 (Catalog #AAA6432118) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The POLD1 (DNA Polymerase delta Catalytic Subunit 125kD, CDC2, MDPL, POLD, CRCS10) (APC) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's POLD1 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC). IF: 1:50-1:200 WB: 1:500-1:2000 IHC: 1:50-1:200 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the POLD1 pold1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "POLD1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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