Human HRG/HPRG ELISA Kit | HRG/HPRG elisa kit

Human HRG/HPRG PicoKine ELISA Kit

Cat. Num. AAA1751609

• Gene Names: HRG; HPRG; HRGP; THPH11
• Reactivity: Human
• Synonyms: HRG/HPRG; Human HRG/HPRG PicoKine ELISA Kit; Human HRG; HRG/HPRG elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human HP
• Sequence Length: 525

• Samples: Cell Culture Supernates, Serum And Plasma (Heparin, Edta)
• Detection Range: 3.12ng/ml-200ng/ml
• Sensitivity: <20pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision
• Inter-assay Precision: Inter-Assay Precision (Precision accross assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HRG/HPRG elisa kit

Principle of the Assay: The Picokine Human HP Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human HP with a 96-well strip plate that is pre-coated with antibody specific for HP. The detection antibody is a biotinylated antibody specific for HP. The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated polyclonal antibody from goat. The kit contains recombinant Human HP with immunogen: Expression system for standard: NSO, Immunogen sequence: V19-N406. The kit is analytically validated with ready to use reagents. To measure Human HP, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly propotional to Human HP in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human HP in the sample!!Background/Introduction: Haptoglobin(HP), is a protein that in humans is encoded by the HP gene. Haptoglobin, a plasma glycoprotein that binds free hemoglobin, has a tetrameric structure of 2 alpha and 2 beta polypeptides that are covalently associated by disulfide bonds. Haptoglobin is homologous to serine proteases of the chymotrypsinogen family. In the chimpanzee, there are 3 genes in the haptoglobin family(haptoglobin, HP; haptoglobin-related, HPR; and haptoglobin-primate, HPP), whereas only 2 genes exist in humans(HP and HPR). There are similarities between the primary structures of the alpha chain and of light chains of gamma globulins. The alpha haptoglobin locus is on the long arm of chromosome 16, where its exact cytogenetic location is 16q22.2. A major function of haptoglobin is to bind hemoglobin(Hb) to form a stable Hp-Hb complex and thereby prevent Hb-induced oxidative tissue damage. Haptoglobin is an unusual secretory protein in that it is proteolytically processed in the endoplasmic reticulum and not in the Golgi. The human haptoglobin HP*2 allele contains a 1.7-kb intragenic duplication that arose after a unique nonhomologous recombination between the prototype HP*1 alleles.

NCBI and Uniprot Product Information

• NCBI GI #: 4504489
• NCBI GeneID: 3273
• NCBI Accession #: NP_000403.1
• NCBI GenBank Nucleotide #: NM_000412.4
• UniProt Accession #: P04196
• NCBI Official Full Name: histidine-rich glycoprotein
• NCBI Official Synonym Full Names: histidine rich glycoprotein
• NCBI Official Symbol: HRG
• NCBI Official Synonym Symbols: HPRG; HRGP; THPH11
• NCBI Protein Information: histidine-rich glycoprotein

NCBI Description

This histidine-rich glycoprotein contains two cystatin-like domains and is located in plasma and platelets. The physiological function has not been determined but it is known that the protein binds heme, dyes and divalent metal ions. The encoded protein also has a peptide that displays antimicrobial activity against C. albicans, E. coli, S. aureus, P. aeruginosa, and E. faecalis. It can inhibit rosette formation and interacts with heparin, thrombospondin and plasminogen. Two of the protein's effects, the inhibition of fibrinolysis and the reduction of inhibition of coagulation, indicate a potential prothrombotic effect. Mutations in this gene lead to thrombophilia due to abnormal histidine-rich glycoprotein levels. [provided by RefSeq, Nov 2014]