Western Blot (WB)
(Western Blot analysis in mouse liver tissue lysates (15ug/lane) using MBS6002835. This demonstrates that MBS6002835 detected RTKN2 protein (arrow).)
Rabbit anti-Human, Mouse RTKN2 Polyclonal Antibody | anti-RTKN2 antibody
RTKN2, NT (RTKN2, PLEKHK1, Rhotekin-2, Pleckstrin homology domain-containing family K member 1)
Gene Names
RTKN2; PLEKHK1; bA531F24.1
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Flow Cytometry, Functional Assay
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
RTKN2; Polyclonal Antibody; NT (RTKN2; PLEKHK1; Rhotekin-2; Pleckstrin homology domain-containing family K member 1); Anti -RTKN2; anti-RTKN2 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Human, mouse
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Applicable Applications for anti-RTKN2 antibody
ELISA (EL/EIA), Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes
Suitable for use in Western Blot, Flow Cytometry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Flow Cytometry: 1:10-50
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Flow Cytometry: 1:10-50
Immunogen
RTKN2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 106-135 amino acids from the N-terminal region of human RTKN2.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot analysis in mouse liver tissue lysates (15ug/lane) using MBS6002835. This demonstrates that MBS6002835 detected RTKN2 protein (arrow).)
Western Blot (WB)
(Western Blot analysis in mouse liver tissue lysates (15ug/lane) using MBS6002835. This demonstrates that MBS6002835 detected RTKN2 protein (arrow).)
Flow Cytometry (FC/FACS)
(Flow Cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram) using MBS6002835. FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(Flow Cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram) using MBS6002835. FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-RTKN2 antibody
RTKN2 may play an important role in lymphopoiesis.
Product Categories/Family for anti-RTKN2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
69,304 Da
NCBI Official Full Name
rhotekin-2 isoform 2
NCBI Official Synonym Full Names
rhotekin 2
NCBI Official Symbol
RTKN2
NCBI Official Synonym Symbols
PLEKHK1; bA531F24.1
NCBI Protein Information
rhotekin-2; PH domain-containing family K member 1; pleckstrin homology domain-containing family K member 1; pleckstrin homology domain containing, family K member 1
UniProt Protein Name
Rhotekin-2
Protein Family
UniProt Gene Name
RTKN2
UniProt Synonym Gene Names
PLEKHK1; PH domain-containing family K member 1
UniProt Entry Name
RTKN2_HUMAN
Uniprot Description
RTKN2: a Rho-GTPase effector protein with a PH domain. Expressed in CD4+ T-cells and bone marrow-derived B-cells. Gene expression was increased in quiescent T-cells and negligible in activated proliferating cells. Expression was absent in myeloid leukaemias, low in most B-cell malignancies and CD8+ T-cell malignancies, but very high in double positive T-lymphoblastic lymphoma. Three alternatively spliced variants have been reported in human samples.
Protein type: Unknown function
Chromosomal Location of Human Ortholog: 10q21.2
Cellular Component: plasma membrane
Biological Process: positive regulation of cell proliferation; hemopoiesis; signal transduction
Research Articles on RTKN2
1. we identified two gene loci associated with rheumatoid arthritis susceptibility- NFKBIE and RTKN2
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Product Notes
The RTKN2 rtkn2 (Catalog #AAA6002835) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RTKN2, NT (RTKN2, PLEKHK1, Rhotekin-2, Pleckstrin homology domain-containing family K member 1) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's RTKN2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Flow Cytometry (FC/FACS). Suitable for use in Western Blot, Flow Cytometry, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500 Flow Cytometry: 1:10-50. Researchers should empirically determine the suitability of the RTKN2 rtkn2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RTKN2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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