Western Blot (WB)
(Anti-PFKFB4 rabbit polyclonal antibody at 1:500 dilution Lane A: U-251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:54 kDa Observed band size:54 kDa)
Rabbit anti-Human PFKFB4 Polyclonal Antibody | anti-PFKFB4 antibody
Anti-PFKFB4 Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Purity
Protein A & Antigen Affinity
Synonyms
PFKFB4; Polyclonal Antibody; Anti-PFKFB4 Antibody; Rabbit Polyclonal; PFKFB4 Antibody; Rabbit PAb; Antigen Affinity Purified; 6-phosphofructo-2-kinase/fructose-2; 6-biphosphatase 4; anti-PFKFB4 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human PFKFB4
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-PFKFB4 antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:2000
IP: 5-10uL/mg of lysate
IP: 5-10uL/mg of lysate
Immunogen
E Coli-derived Human PFKFB4 fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human PFKFB4 fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-PFKFB4 rabbit polyclonal antibody at 1:500 dilution Lane A: U-251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:54 kDa Observed band size:54 kDa)
Western Blot (WB)
(Anti-PFKFB4 rabbit polyclonal antibody at 1:500 dilution Lane A: U-251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:54 kDa Observed band size:54 kDa)
Immunoprecipitation (IP)
(PFKFB4 was immunoprecipitated using: Lane A:0.5 mg U-251 MG Whole Cell Lysate 4 uL anti-PFKFB4 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-PFKFB4 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 54 kDa Observed band size :55 kDa)
Immunoprecipitation (IP)
(PFKFB4 was immunoprecipitated using: Lane A:0.5 mg U-251 MG Whole Cell Lysate 4 uL anti-PFKFB4 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-PFKFB4 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 54 kDa Observed band size :55 kDa)
Related Product Information for anti-PFKFB4 antibody
The bi-functional enzyme 6-phosphofructo-2-kinase/fructose-2, 6-biphosphatase-4 (PFKFB4) is highly expressed in many types of cancer and its requirement for tumor survival has been demonstrated in glioma, lung, and prostate cancers. PFKFB4 is induced by hypoxia and is strongly associated with glycolysis. PFKFB4, a regulatory enzyme that synthesizes a potent stimulator of glycolysis 2, is found to be a robust stimulator of SRC-3 that co-regulates the oestrogen receptor. PFKFB4 phosphorylates SRC-3 at serine 857 and enhances its transcriptional activity, whereas either suppression of PFKFB4 or ectopic expression of a phosphorylation-deficient Ser857Ala mutant SRC-3 abolishes the SRC-3-mediated transcriptional output. Functionally, PFKFB4-driven SRC-3 activation drives glucose flux towards the pentose phosphate pathway and enables purine synthesis by transcriptionally upregulating the expression of the enzyme transketolase.
References
Yun SJ, et al. (2012) Pfkfb4 as a prognostic marker in non-muscle-invasive bladder cancer. Urol Oncol 30 (6): 893-899.Gao R, et al. (2018) Pfkfb4 promotes breast cancer metastasis via induction of hyaluronan production in a p38-dependent manner. Cell Physiol Biochem 50 (6): 2108-2123.Dasgupta S, et al. (2018) Metabolic enzyme pfkfb4 activates transcriptional coactivator src-3 to drive breast cancer. Nature 556 (7700): 249-254.
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Product Notes
The PFKFB4 (Catalog #AAA8108906) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PFKFB4 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PFKFB4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). WB: 1:500-1:2000 IP: 5-10uL/mg of lysate. Researchers should empirically determine the suitability of the PFKFB4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PFKFB4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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