Rabbit anti-Human IFT20 Polyclonal Antibody | anti-IFT20 antibody

Anti-IFT20 Antibody, Rabbit Polyclonal

Cat. Num. AAA8109293

• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: IFT20; Polyclonal Antibody; Anti-IFT20 Antibody; Rabbit Polyclonal; IFT20/intraflagellar transport 20 Antibody; Rabbit PAb; Antigen Affinity Purified; intraflagellar transport 20; anti-IFT20 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human IFT20/intraflagellar transport 20
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-IFT20 antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; IHC-P: 1:50-1:200; IP: 5-10uL/mg of lysate
• Immunogen: E Coli-derived Human IFT20/intraflagellar transport 20 fragment
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with E Coli-derived Human IFT20/intraflagellar transport 20 fragment, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-IFT20 rabbit polyclonal antibody at 1:500 dilution Lane A: U-251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:15 kDa Observed band size:17 kDa)

Immunohistochemistry (IHC)

(Immunochemical staining of human IFT20 in human adrenal gland with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

Immunoprecipitation (IP)

(IFT20 was immunoprecipitated using: Lane A:0.5 mg U-251 MG Whole Cell Lysate 4 uL anti-IFT20 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-IFT20 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 15 kDa Observed band size :15 kDa)

Related Product Information for anti-IFT20 antibody

Eukaryotic cilia are assembled via intraflagellar transport (IFT) in which large protein particles motor along ciliary microtubules. IFT20 functions in the delivery of ciliary membrane proteins from the Golgi complex to the cilium. IFT20 is the smallest member of the intraflagellar transport protein (IFT) complex B. It is involved in cilia formation. IFT20 in the early stage of T-cell development inhibited collagen-induced arthritis (CIA) development through regulating T-cell development and the expression of critical cytokines. The intraflagellar transport protein 20 (IFT20), a component of the intraflagellar transport system, controls polarized traffic during immune synapse assembly. IFT20 is therefore required for the delivery of the intracellular pool of LAT to the immune synapse in naive primary T lymphocytes and for effective T-cell responses in vivo. IFT20 is essential for male fertility and spermiogenesis in mice, and its major function is to transport cargo proteins for sperm flagella formation.