Western Blot (WB)
(Gel: 8%SDS-PAGE Lysate: 40 μ g Lane: Raji cell Primary antibody: 1/300 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 1 minute)
Rabbit anti-Human PFKFB4 Polyclonal Antibody | anti-PFKFB4 antibody
PFKFB4 Antibody
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Antigen Affinity Purification
Synonyms
PFKFB4; Polyclonal Antibody; PFKFB4 Antibody; 6-phosphofructo-2-kinase; 6-phosphofructo-2-kinase/fructose-2; 6-biphosphatase 4; 6-biphosphatase-4; 6PF-2-K/Fru-2; 6-P2ase 4; anti-PFKFB4 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody detects endogenous levels of total PFKFB4 protein.
Purity/Purification
Antigen Affinity Purification
Form/Format
Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
Concentration
1.4 mg/ml (varies by lot)
Sequence Length
498
Applicable Applications for anti-PFKFB4 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Western Blot: 1:200-1:1000
Immunohistochemistry: 1:25-1:100
Immunohistochemistry: 1:25-1:100
Immunogen
Synthetic peptide of human PFKFB4
Immunogen Type
Peptide
Preparation and Storage
Store at -20 degree C
Western Blot (WB)
(Gel: 8%SDS-PAGE Lysate: 40 μ g Lane: Raji cell Primary antibody: 1/300 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 1 minute)
Western Blot (WB)
(Gel: 8%SDS-PAGE Lysate: 40 μ g Lane: Raji cell Primary antibody: 1/300 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 1 minute)
Related Product Information for anti-PFKFB4 antibody
6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4 also known as PFKFB4 is an enzyme which in humans is encoded by the PFKFB4 gene. The bifunctional 6-phosphofructo-2-kinase (EC 2.7.1.105)/fructose-2,6-bisphosphatase (EC 3.1.3.46) (PFKFB) regulates the steady-state concentration of fructose 2,6-bisphosphate, an activator of a key regulatory enzyme of glycolysis, phosphofructokinase.
Product Categories/Family for anti-PFKFB4 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
50,203 Da
NCBI Official Full Name
6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4 isoform a
NCBI Official Synonym Full Names
6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4
NCBI Official Symbol
PFKFB4
NCBI Protein Information
6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4
UniProt Protein Name
6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4
Protein Family
UniProt Gene Name
PFKFB4
UniProt Synonym Gene Names
6PF-2-K/Fru-2,6-P2ase 4; PFK/FBPase 4
UniProt Entry Name
F264_HUMAN
Uniprot Description
PFKFB4: Synthesis and degradation of fructose 2,6-bisphosphate.
Protein type: Carbohydrate Metabolism - fructose and mannose; EC 2.7.1.105; EC 3.1.3.46; Kinase, other; Motility/polarity/chemotaxis; Phosphatase (non-protein)
Chromosomal Location of Human Ortholog: 3p22-p21
Cellular Component: cytosol
Molecular Function: 6-phosphofructo-2-kinase activity
Similar Products
Product Notes
The PFKFB4 pfkfb4 (Catalog #AAA9415632) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PFKFB4 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PFKFB4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Western Blot: 1:200-1:1000 Immunohistochemistry: 1:25-1:100. Researchers should empirically determine the suitability of the PFKFB4 pfkfb4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PFKFB4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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