Western Blot (WB)
(Western Blot (WB) analysis of Pepsin C (F306) pAb at 1:500 dilutionLane1:HepG2 whole cell lysate (40ug) Lane2:LO2 whole cell lysate (40ug) Lane3:SGC7901 whole cell lysate (40ug) Lane4:PMVEC whole cell lysate (40ug) Lane5:AML-12 whole cell lysate (40ug))
Rabbit Pepsin C Polyclonal Antibody | anti-F306 antibody
Pepsin C (F306) Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
Pepsin C; Polyclonal Antibody; Pepsin C (F306) Polyclonal Antibody; Pepsinogen C; PGC; Gastricsin; anti-F306 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Pepsin C (F306) polyclonal antibody detects endogenous levels of Pepsin C protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
388
Applicable Applications for anti-F306 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:1000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
Synthetic peptide, corresponding to amino acids 280-330 of Human Pepsin C.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot (WB) analysis of Pepsin C (F306) pAb at 1:500 dilutionLane1:HepG2 whole cell lysate (40ug) Lane2:LO2 whole cell lysate (40ug) Lane3:SGC7901 whole cell lysate (40ug) Lane4:PMVEC whole cell lysate (40ug) Lane5:AML-12 whole cell lysate (40ug))
Western Blot (WB)
(Western Blot (WB) analysis of Pepsin C (F306) pAb at 1:500 dilutionLane1:HepG2 whole cell lysate (40ug) Lane2:LO2 whole cell lysate (40ug) Lane3:SGC7901 whole cell lysate (40ug) Lane4:PMVEC whole cell lysate (40ug) Lane5:AML-12 whole cell lysate (40ug))
Immunohistochemistry (IHC)
(Immunohistochemistry (IHC) analyzes of Pepsin C (F306) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.)
Immunohistochemistry (IHC)
(Immunohistochemistry (IHC) analyzes of Pepsin C (F306) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.)
Related Product Information for anti-F306 antibody
Pepsin is one of the main proteolytic enzymes secreted by the gastric mucosa. Pepsin consists of a single polypeptide chain and arises from its precursor, pepsinogen, by removal of a 41 amino acid segment from the N-terminus. Pepsinogen is synthesized in the stomach lining, and hydrochloric acid, also produced by the gastric mucosa, is necessary to convert the inactive enzyme and to maintain the optimum acidity (pH 1-3) for pepsin function. Pepsin is particularly effective in cleaving peptide bonds involving aromatic amino acids. Pepsin shows extremely broad specificity, and although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent. The amino acid composition of Pepsin C differs from those of pepsinogen and pepsin especially in the content of basic amino acids, glutamic acid, aspartic acid, leucine and isoleucine.
NCBI and Uniprot Product Information
Uniprot Description
PGC: Hydrolyzes a variety of proteins. Belongs to the peptidase A1 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted, signal peptide; Protease; Secreted; EC 3.4.23.3
Chromosomal Location of Human Ortholog: 6p21.1
Cellular Component: extracellular space
Molecular Function: aspartic-type endopeptidase activity
Biological Process: digestion; proteolysis
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Product Notes
The F306 pgc (Catalog #AAA3004142) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Pepsin C (F306) Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Pepsin C can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:1000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the F306 pgc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Pepsin C, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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