Rabbit IL-32 Polyclonal Antibody | anti-IL-32 antibody

IL-32 (K226) Polyclonal Antibody

Cat. Num. AAA3004126

• Gene Names: IL32; NK4; TAIF; TAIFa; TAIFb; TAIFc; TAIFd; IL-32beta; IL-32alpha; IL-32delta; IL-32gamma
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: IL-32; Polyclonal Antibody; IL-32 (K226) Polyclonal Antibody; Interleukin-32; Natural killer cells protein 4; Tumor necrosis factor alpha-inducing factor; NK4; TAIF; IL32; anti-IL-32 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: IL-32 (K226) polyclonal antibody detects endogenous levels of IL-32 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 188

• Applicable Applications for anti-IL-32 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 200-2450 of Human IL-32.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of IL-32 (K226) pAb at 1:500 dilutionLane1:HepG2 whole cell lysate (40ug) Lane2:LO2 whole cell lysate (20ug) Lane3:SGC7901 whole cell lysate (40ug) Lane4:PMVEC whole cell lysate (40ug) Lane5:AML-12 whole cell lysate (40ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of IL-32 (K226) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.)

Related Product Information for anti-IL-32 antibody

Interleukin-32 (IL-32), also designated Natural Killer (NK) cell transcript 4 (nk4), may play a role in inflammatory responses such as lymphocyte activation. IL-32 is secreted following stimulation with inflammatory cytokines such as IL-1beta and IFN-gamma, and by NK cells after exposure to IL-12 and IL-18. It also induces production of IL-1beta, TNFalpha, MIP-2 and IL-6. Expression of IL-32 increases after activation of T cells by mitogens or activation of NK cells by IL-2. The IL-32 protein contains three potential N-myristoylation sites, a tyrosine sulfation site, an RGD cellattachment sequence and multiple putative phosphorylation sites. Human IL-32 exists as four isoforms.

NCBI and Uniprot Product Information

• NCBI GI #: 61658642
• NCBI GeneID: 9235
• NCBI Accession #: NP_001012736.1
• NCBI GenBank Nucleotide #: NP_001012736.1
• UniProt Accession #: P24001
• Molecular Weight: ~ 27kDa
• NCBI Official Full Name: interleukin-32 isoform B
• NCBI Official Synonym Full Names: interleukin 32
• NCBI Official Symbol: IL32
• NCBI Official Synonym Symbols: NK4; TAIF; TAIFa; TAIFb; TAIFc; TAIFd; IL-32beta; IL-32alpha; IL-32delta; IL-32gamma
• NCBI Protein Information: interleukin-32
• UniProt Protein Name: Interleukin-32
• UniProt Gene Name: IL32
• UniProt Synonym Gene Names: NK4; TAIF; IL-32
• UniProt Entry Name: IL32_HUMAN

NCBI Description

This gene encodes a member of the cytokine family. The protein contains a tyrosine sulfation site, 3 potential N-myristoylation sites, multiple putative phosphorylation sites, and an RGD cell-attachment sequence. Expression of this protein is increased after the activation of T-cells by mitogens or the activation of NK cells by IL-2. This protein induces the production of TNFalpha from macrophage cells. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

IL32: Cytokine that may play a role in innate and adaptive immune responses. It induces various cytokines such as TNFA/TNF- alpha and IL8. It activates typical cytokine signal pathways of NF-kappa-B and p38 MAPK. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: extracellular space; membrane

Molecular Function: protein binding; cytokine activity

Biological Process: defense response; immune response; cell adhesion