Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human stomach cancer tissues using PGA5 antibody with DAB staining.)
Mouse PGA5 Monoclonal Antibody | anti-PGA5 antibody
PGA5 antibody
Gene Names
PGA5; Pg5
Applications
ELISA, Immunohistochemistry, Western Blot
Synonyms
PGA5; Monoclonal Antibody; PGA5 antibody; Mouse monoclonal PGA5 antibody; Monoclonal PGA5 antibody; Anti-PGA5 antibody; Pepsinogen 5 group I antibody; Pepsinogen A antibody; PGA 5; PGA-5; PGA 5 antibody; PGA-5 antibody; anti-PGA5 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
2C1
Form/Format
Liquid in ascitic fluid containing 0.03% sodium azide.
Concentration
1.0 mg/ml (varies by lot)
Sequence Length
388
Applicable Applications for anti-PGA5 antibody
ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
ELISA: 1:10,000
IHC: 1:200-1:1000
WB: 1:500-1:2000
IHC: 1:200-1:1000
WB: 1:500-1:2000
Immunogen
PGA5 antibody was raised in Mouse using a purified recombinant fragment of human PGA5 expressed in E Coli as the immunogen.
Cross-Reactivity
Human
Preparation and Storage
Store at 4 degree C for short term storage.
Store at-20 degree C for long term storage.
Avoid repeated freeze/thaw cycles.
Store at-20 degree C for long term storage.
Avoid repeated freeze/thaw cycles.
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human stomach cancer tissues using PGA5 antibody with DAB staining.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human stomach cancer tissues using PGA5 antibody with DAB staining.)
Western Blot (WB)
(Western Blot showing PGA5 antibody used against HepG2 (1) and SMMC-7721 (2) cell lysate.)
Western Blot (WB)
(Western Blot showing PGA5 antibody used against HepG2 (1) and SMMC-7721 (2) cell lysate.)
Related Product Information for anti-PGA5 antibody
Pepsinogens are the inactive precursors of pepsin, the major acid protease found in the stomach. Pepsin is one of the main proteolytic enzymes secreted by the gastric mucosa. Pepsin consists of a single polypeptide chain and arises from its precursor, pepsinogen, by removal of a 41 amino acid segment from the N-terminus. Pepsin is particularly effective in cleaving peptide bonds involving aromatic amino acids. Pepsin shows extremely broad specificity; although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent. PGA5 is a member of the subfamily A1 within the pepsin family and is the predominant endopeptidase in the gastric juice of vertebrates.
Product Categories/Family for anti-PGA5 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
41,993 Da
NCBI Official Full Name
pepsin A-5 preproprotein
NCBI Official Synonym Full Names
pepsinogen 5, group I (pepsinogen A)
NCBI Official Symbol
PGA5
NCBI Official Synonym Symbols
Pg5
NCBI Protein Information
pepsin A-5
UniProt Protein Name
Pepsin A-5
Protein Family
UniProt Gene Name
PGA5
NCBI Description
This gene encodes a protein precursor of the digestive enzyme pepsin, a member of the peptidase A1 family of endopeptidases. The encoded precursor is secreted by gastric chief cells and undergoes autocatalytic cleavage in acidic conditions to form the active enzyme, which functions in the digestion of dietary proteins. This gene is found in a cluster of related genes on chromosome 11, each of which encodes one of multiple pepsinogens. Pepsinogen levels in serum may serve as a biomarker for atrophic gastritis and gastric cancer. [provided by RefSeq, Jul 2015]
Uniprot Description
Shows particularly broad specificity; although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent.
Research Articles on PGA5
Similar Products
Product Notes
The PGA5 pga5 (Catalog #AAA5310880) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's PGA5 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB). ELISA: 1:10,000 IHC: 1:200-1:1000 WB: 1:500-1:2000. Researchers should empirically determine the suitability of the PGA5 pga5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PGA5, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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