Rabbit anti-Human PEPCK2 Polyclonal Antibody | anti-PCK2 antibody

PEPCK2 (Phosphoenolpyruvate Carboxykinase [GTP], Mitochondrial, PEPCK-M, Phosphoenolpyruvate Carboxylase, PCK2)

Cat. Num. AAA646670

• Gene Names: PCK2; PEPCK; PEPCK2; PEPCK-M
• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: PEPCK2; Polyclonal Antibody; PEPCK2 (Phosphoenolpyruvate Carboxykinase [GTP]; Mitochondrial; PEPCK-M; Phosphoenolpyruvate Carboxylase; PCK2); Anti -PEPCK2 (Phosphoenolpyruvate Carboxykinase [GTP]; anti-PCK2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PCK2.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2.
• Sequence: MAALYRPGLRLNWHGLSPLGWPSCRSIQTLRVLSGDLGQLPTGIRDFVEHSARLCQPEGIHICDGTEAENTATLTLLEQQGLIRKLPKYNNCWLARTDPKDVARVESKTVIVTPSQRDTVPLPPGGARGQLGNWMSPADFQRAVDERFPGCMQGRTMYVLPFSMGPVGSPLSRIGVQLTDSAYVVASMRIMTRLGTPVLQALGDGDFVKCLHSVGQPLTGQGEPVSQWPCNPEKTLIGHVPDQREIISFGSGYGGNSLLGKKCFALRIASRLARDEGWLAEHMLILGITSPAGKKRYVAAAFPSACGKTNLAMMRPALPGWKVECVGDDIAWMRFDSEGRLRAINPENGFFGVAPGTSATTNPNAMATIQSNTIFTNVAETSDGGVYWEGIDQPLPPGVTVTSWLGKPWKPGDKEPCAHPNSRFCAPARQCPIMDPAWEAPEGVPIDAIIFGGRRPKGVPLVYEAFNWRHGVFVGSAMRSESTAAAEHKGKIIMHDPFAMRPFFGYNFGHYLEHWLSMEGRKGAQLPRIFHVNWFRRDEAGHFLWPGFGENARVLDWICRRLEGEDSARETPIGLVPKEGALDLSGLRAIDTTQLFSLPKDFWEQEVRDIRSYLTEQVNQDLPKEVLAELEALERRVHKM

• Applicable Applications for anti-PCK2 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.
• Immunogen: Full length human PCK2, aa1-640 (AAH01454.1).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(PCK2 rabbit polyclonal antibody. Western Blot analysis of PCK2 expression in human liver.)

Western Blot (WB)

(PCK2 rabbit polyclonal antibody. Western Blot analysis of PCK2 expression in HepG2.)

Western Blot (WB)

(Western Blot analysis of PCK2 expression in transfected 293T cell line by PCK2 polyclonal antibody. Lane 1: PCK2 transfected lysate (70.7kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-PCK2 antibody

This gene encodes a member of the phosphoenolpyruvate carboxykinase (GTP) family. The protein is a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of GTP. A cytosolic form encoded by a different gene has also been characterized and is the key enzyme of gluconeogenesis in the liver. The encoded protein may serve a similar function, although it is constitutively expressed and not modulated by hormones such as glucagon and insulin that regulate the cytosolic form. Alternatively spliced transcript variants have been described.

Product Categories/Family for anti-PCK2 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 66346723
• NCBI GeneID: 5106
• NCBI Accession #: NP_001018083.1
• NCBI GenBank Nucleotide #: NM_001018073.1
• UniProt Accession #: Q16822; O43253; Q86U01; Q9BV62
• Molecular Weight: 70,730 Da
• NCBI Official Full Name: phosphoenolpyruvate carboxykinase
• NCBI Official Synonym Full Names: phosphoenolpyruvate carboxykinase 2 (mitochondrial)
• NCBI Official Symbol: PCK2
• NCBI Official Synonym Symbols: PEPCK; PEPCK2; PEPCK-M
• NCBI Protein Information: phosphoenolpyruvate carboxykinase [GTP], mitochondrial; phosphoenolpyruvate carboxykinase [GTP], mitochondrial; PEP carboxykinase; phosphopyruvate carboxylase; phosphoenolpyruvate carboxylase
• UniProt Protein Name: Phosphoenolpyruvate carboxykinase [GTP], mitochondrial
• Protein Family: Protein kinase
• UniProt Gene Name: PCK2
• UniProt Synonym Gene Names: PEPCK2; PEPCK-M
• UniProt Entry Name: PCKGM_HUMAN

NCBI Description

This gene encodes a member of the phosphoenolpyruvate carboxykinase (GTP) family. The protein is a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of GTP. A cytosolic form encoded by a different gene has also been characterized and is the key enzyme of gluconeogenesis in the liver. The encoded protein may serve a similar function, although it is constitutively expressed and not modulated by hormones such as glucagon and insulin that regulate the cytosolic form. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle

By similarity.

Catalytic activity: GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.

Cofactor: Manganese

By similarity.Binds 1 manganese ion per subunit

By similarity.

Pathway: Carbohydrate biosynthesis; gluconeogenesis.

Subunit structure: Monomer.

Subcellular location: Mitochondrion.

Involvement in disease: Mitochondrial phosphoenolpyruvate carboxykinase deficiency (M-PEPCKD) [MIM:261650]: Metabolic disorder resulting from impaired gluconeogenesis. It is a rare disease with less than 10 cases reported in the literature. Clinical characteristics include hypotonia, hepatomegaly, failure to thrive, lactic acidosis and hypoglycemia. Autopsy reveals fatty infiltration of both the liver and kidneys. The disorder is transmitted as an autosomal recessive trait.Note: The disease is caused by mutations affecting the gene represented in this entry.

Miscellaneous: In eukaryotes there are two isozymes: a cytoplasmic one and a mitochondrial one.

Sequence similarities: Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.

Sequence caution: The sequence CAD62600.1 differs from that shown. Reason: Erroneous initiation.