Western Blot (WB)
(PCK2 rabbit polyclonal antibody. Western Blot analysis of PCK2 expression in human liver.)
Rabbit anti-Human PEPCK2 Polyclonal Antibody | anti-PEPCK2 antibody
PEPCK2 (Phosphoenolpyruvate Carboxykinase [GTP], Mitochondrial, PEPCK-M, Phosphoenolpyruvate Carboxylase, PCK2) (AP)
Gene Names
PCK2; PEPCK; PEPCK2; PEPCK-M
Reactivity
Human
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
PEPCK2; Polyclonal Antibody; PEPCK2 (Phosphoenolpyruvate Carboxykinase [GTP]; Mitochondrial; PEPCK-M; Phosphoenolpyruvate Carboxylase; PCK2) (AP); anti-PEPCK2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human PCK2.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Applicable Applications for anti-PEPCK2 antibody
Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
Full length human PCK2, aa1-640 (AAH01454.1).
Immunogen Sequence
MAALYRPGLRLNWHGLSPLGWPSCRSIQTLRVLSGDLGQLPTGIRDFVEHSARLCQPEGIHICDGTEAENTATLTLLEQQGLIRKLPKYNNCWLARTDPKDVARVESKTVIVTPSQRDTVPLPPGGARGQLGNWMSPADFQRAVDERFPGCMQGRTMYVLPFSMGPVGSPLSRIGVQLTDSAYVVASMRIMTRLGTPVLQALGDGDFVKCLHSVGQPLTGQGEPVSQWPCNPEKTLIGHVPDQREIISFGSGYGGNSLLGKKCFALRIASRLARDEGWLAEHMLILGITSPAGKKRYVAAAFPSACGKTNLAMMRPALPGWKVECVGDDIAWMRFDSEGRLRAINPENGFFGVAPGTSATTNPNAMATIQSNTIFTNVAETSDGGVYWEGIDQPLPPGVTVTSWLGKPWKPGDKEPCAHPNSRFCAPARQCPIMDPAWEAPEGVPIDAIIFGGRRPKGVPLVYEAFNWRHGVFVGSAMRSESTAAAEHKGKIIMHDPFAMRPFFGYNFGHYLEHWLSMEGRKGAQLPRIFHVNWFRRDEAGHFLWPGFGENARVLDWICRRLEGEDSARETPIGLVPKEGALDLSGLRAIDTTQLFSLPKDFWEQEVRDIRSYLTEQVNQDLPKEVLAELEALERRVHKM
Conjugate
AP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(PCK2 rabbit polyclonal antibody. Western Blot analysis of PCK2 expression in human liver.)
Western Blot (WB)
(PCK2 rabbit polyclonal antibody. Western Blot analysis of PCK2 expression in human liver.)
Western Blot (WB)
(PCK2 rabbit polyclonal antibody. Western Blot analysis of PCK2 expression in HepG2.)
Western Blot (WB)
(PCK2 rabbit polyclonal antibody. Western Blot analysis of PCK2 expression in HepG2.)
Western Blot (WB)
(Western Blot analysis of PCK2 expression in transfected 293T cell line by PCK2 polyclonal antibody. Lane 1: PCK2 transfected lysate (70.7kD). Lane 2: Non-transfected lysate.)
Western Blot (WB)
(Western Blot analysis of PCK2 expression in transfected 293T cell line by PCK2 polyclonal antibody. Lane 1: PCK2 transfected lysate (70.7kD). Lane 2: Non-transfected lysate.)
Related Product Information for anti-PEPCK2 antibody
This gene encodes a member of the phosphoenolpyruvate carboxykinase (GTP) family. The protein is a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of GTP. A cytosolic form encoded by a different gene has also been characterized and is the key enzyme of gluconeogenesis in the liver. The encoded protein may serve a similar function, although it is constitutively expressed and not modulated by hormones such as glucagon and insulin that regulate the cytosolic form. Alternatively spliced transcript variants have been described.
Product Categories/Family for anti-PEPCK2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
47,564 Da
NCBI Official Full Name
Homo sapiens phosphoenolpyruvate carboxykinase 2 (mitochondrial), mRNA
NCBI Official Synonym Full Names
phosphoenolpyruvate carboxykinase 2, mitochondrial
NCBI Official Symbol
PCK2
NCBI Official Synonym Symbols
PEPCK; PEPCK2; PEPCK-M
NCBI Protein Information
phosphoenolpyruvate carboxykinase [GTP], mitochondrial
Protein Family
NCBI Description
This gene encodes a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of guanosine triphosphate (GTP). A cytosolic form of this protein is encoded by a different gene and is the key enzyme of gluconeogenesis in the liver. Alternatively spliced transcript variants have been described. [provided by RefSeq, Apr 2014]
Research Articles on PEPCK2
Similar Products
Product Notes
The PEPCK2 (Catalog #AAA6389031) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PEPCK2 (Phosphoenolpyruvate Carboxykinase [GTP], Mitochondrial, PEPCK-M, Phosphoenolpyruvate Carboxylase, PCK2) (AP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PEPCK2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the PEPCK2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PEPCK2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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