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Western Blot (WB)
(Western Blot analysis in HL-60 cell line lysates (35ug/lane) using MBS6001893. This demonstrates that MBS6001893 detected the SPECC1 protein (arrow).)
Rabbit anti-Human, Mouse SPECC1 Polyclonal Antibody
SPECC1, ID (SPECC1L, CYTSA, KIAA0376, Cytospin-A, Renal carcinoma antigen NY-REN-22, Sperm antigen with calponin homology and coiled-coil domains 1-like)
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay, Immunofluorescence
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
SPECC1; Polyclonal Antibody; ID (SPECC1L; CYTSA; KIAA0376; Cytospin-A; Renal carcinoma antigen NY-REN-22; Sperm antigen with calponin homology and coiled-coil domains 1-like); Anti -SPECC1; anti-SPECC1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Human, mouse
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Applicable Applications for anti-SPECC1 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
Application Notes
Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:10-50
Flow Cytometry: 1:10-50
Immunofluorescence: 1:10-50
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:10-50
Flow Cytometry: 1:10-50
Immunofluorescence: 1:10-50
Immunogen
SPECC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 712-741 amino acids from the Central region of human SPECC1.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot analysis in HL-60 cell line lysates (35ug/lane) using MBS6001893. This demonstrates that MBS6001893 detected the SPECC1 protein (arrow).)
Western Blot (WB)
(Western Blot analysis in HL-60 cell line lysates (35ug/lane) using MBS6001893. This demonstrates that MBS6001893 detected the SPECC1 protein (arrow).)
Western Blot (WB)
(Western Blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane) using MBS6001893. This demonstrates that MBS6001893 detected the SPECC1 protein (arrow).)
Western Blot (WB)
(Western Blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane) using MBS6001893. This demonstrates that MBS6001893 detected the SPECC1 protein (arrow).)
Testing Data
Testing Data
Flow Cytometry (FC/FACS)
(Flow Cytometry analysis of HL-60 cells using MBS6001893 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(Flow Cytometry analysis of HL-60 cells using MBS6001893 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of U-251MG cells using MBS6001893 followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of U-251MG cells using MBS6001893 followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).)
Related Product Information for anti-SPECC1 antibody
CYTSAis involved in cytokinesis and spindle organization.
Product Categories/Family for anti-SPECC1 antibody
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Product Notes
The SPECC1 (Catalog #AAA6001893) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SPECC1, ID (SPECC1L, CYTSA, KIAA0376, Cytospin-A, Renal carcinoma antigen NY-REN-22, Sperm antigen with calponin homology and coiled-coil domains 1-like) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's SPECC1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunofluorescence (IF). Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:10-50 Flow Cytometry: 1:10-50 Immunofluorescence: 1:10-50. Researchers should empirically determine the suitability of the SPECC1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SPECC1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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