Rabbit anti-Human NLK Polyclonal Antibody | anti-NLK antibody

NLK, CT (Nemo-like Kinase, DKFZp761G1211, FLJ21033, LAK1, Protein LAK1, Serine/threonine Protein Kinase NLK) (APC)

Cat. Num. AAA6490122

• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: NLK; Polyclonal Antibody; CT (Nemo-like Kinase; DKFZp761G1211; FLJ21033; LAK1; Protein LAK1; Serine/threonine Protein Kinase NLK) (APC); EC=2.7.11.24; anti-NLK antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human NLK.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-NLK antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human NLK (KLH).
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze APC conjugates. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-NLK Pab in A375 cell lysate. NLK (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-NLK antibody

Antibodies; Protein Kinases

References

1. Yasuda, J., et al., Biochem. Biophys. Res. Commun. 308(2):227-233 (2003). 2. Ishitani, T., et al., Mol. Cell. Biol. 23(4):1379-1389 (2003). 3. Ishitani, T., et al., Mol. Cell. Biol. 23(1):131-139 (2003). 4. Kehrer-Sawatzki, H., et al., Gene 251(1):63-71 (2000). 5. Brott, B.K., et al., Proc. Natl. Acad. Sci. USA 95(3):963-968 (1998).

NCBI and Uniprot Product Information

• NCBI GI #: 149408126
• NCBI GeneID: 51701
• NCBI Accession #: NP_057315
• NCBI GenBank Nucleotide #: NM_016231
• UniProt Accession #: Q9UBE8; Q2PNI9; Q6P2A3; B2RCX1
• Molecular Weight: 58,283 Da
• NCBI Official Full Name: serine/threonine-protein kinase NLK
• NCBI Official Synonym Full Names: nemo-like kinase
• NCBI Official Symbol: NLK
• NCBI Protein Information: serine/threonine-protein kinase NLK
• UniProt Protein Name: Serine/threonine-protein kinase NLK
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: NLK
• UniProt Entry Name: NLK_HUMAN

Uniprot Description

NLK: Serine/threonine-protein kinase that regulates a number of transcription factors with key roles in cell fate determination. Positive effector of the non-canonical Wnt signaling pathway, acting downstream of WNT5A, MAP3K7/TAK1 and HIPK2. Activation of this pathway causes binding to and phosphorylation of the histone methyltransferase SETDB1. The NLK- SETDB1 complex subsequently interacts with PPARG, leading to methylation of PPARG target promoters at histone H3K9 and transcriptional silencing. The resulting loss of PPARG target gene transcription inhibits adipogenesis and promotes osteoblastogenesis in mesenchymal stem cells (MSCs). Negative regulator of the canonical Wnt/beta-catenin signaling pathway. Binds to and phosphorylates TCF7L2/TCF4 and LEF1, promoting the dissociation of the TCF7L2/LEF1/beta-catenin complex from DNA, as well as the ubiquitination and subsequent proteolysis of LEF1. Together these effects inhibit the transcriptional activation of canonical Wnt/beta-catenin target genes. Negative regulator of the Notch signaling pathway. Binds to and phosphorylates NOTCH1, thereby preventing the formation of a transcriptionally active ternary complex of NOTCH1, RBPJ/RBPSUH and MAML1. Negative regulator of the MYB family of transcription factors. Phosphorylation of MYB leads to its subsequent proteolysis while phosphorylation of MYBL1 and MYBL2 inhibits their interaction with the coactivator CREBBP. Other transcription factors may also be inhibited by direct phosphorylation of CREBBP itself. Acts downstream of IL6 and MAP3K7/TAK1 to phosphorylate STAT3, which is in turn required for activation of NLK by MAP3K7/TAK1. Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.

Protein type: EC 2.7.11.24; Protein kinase, CMGC; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); CMGC group; MAPK family; nmo subfamily

Chromosomal Location of Human Ortholog: 17q11.2

Cellular Component: nucleoplasm; cytosol; nucleus

Molecular Function: MAP kinase activity; protein serine/threonine kinase activity; protein binding; ubiquitin protein ligase binding; magnesium ion binding; SH2 domain binding; transcription factor binding; ATP binding; protein kinase activity

Biological Process: negative regulation of Wnt receptor signaling pathway; Wnt receptor signaling pathway; transcription, DNA-dependent; regulation of transcription, DNA-dependent; transforming growth factor beta receptor signaling pathway; protein amino acid autophosphorylation; MAPKKK cascade; peptidyl-threonine phosphorylation; protein amino acid phosphorylation; serine phosphorylation of STAT3 protein